排序方式: 共有16条查询结果,搜索用时 15 毫秒
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Kathrin Rychli Christoph Kaun Philipp J. Hohensinner Adrian J. Dorfner Stefan Pfaffenberger Alexander Niessner Michael Bauer Wolfgang Dietl Bruno K. Podesser Gerald Maurer Kurt Huber Johann Wojta 《Journal of cellular and molecular medicine》2010,14(1-2):198-205
Cardiac diseases such as myocardial infarction and heart failure are among the leading causes of death in western societies. Therapeutic angiogenesis has been suggested as a concept to combat these diseases. The biology of angiogenic factors expressed in the heart such as vascular endothelial growth factor (VEGF) is well studied, whereas data on anti-angiogenic mediators in the heart are scarce. Here we study the expression of the anti-angiogenic factor pigment epithelium-derived factor (PEDF) in the human heart and in human cardiac cells. PEDF expression could be detected in human cardiac tissue on the protein and mRNA levels. PEDF mRNA levels were significantly lower in explanted human ischemic hearts as compared to healthy hearts. Our in vitro experiments showed that human adult cardiac myocytes and fibroblasts constitutively secrete PEDF. In addition to anoxic conditions, cobalt chloride, 2,2'dipyridyl and dimethoxally glycine, which stabilize hypoxia inducible factor-α decreased PEDF expression. Furthermore we show that PEDF inhibits VEGF-induced sprouting. We have identified PEDF in healthy and ischemic human hearts and we show that PEDF expression is down-regulated by low oxygen levels. Therefore, we suggest a role for PEDF in the regulation of angiogenesis in the heart and propose PEDF as a possible therapeutic target in heart disease. 相似文献
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Thirty-five desert cottontails (Sylvilagus audubonii) and 35 black-tailed jack rabbits (Lepus californicus), occurring sympatrically near the Clovis-Portales area of eastern New Mexico were infected with four species of Eucestoda (adults of Raillietina salmoni and Raillietina selfi, larvae of Taenia pisiformis and Taenia serialis). Raillietina salmoni and T. pisiformis more commonly infected S. audubonii. Raillietina selfi was found in near equal prevalence in both host species. Taenia serialis was recovered only from L. californicus. Thus, three of the four helminth species were shared by both lagomorphs (Jaccard's coefficient = 75). Female hosts were most heavily infected with R. selfi and Taenia serialis. 相似文献
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C.D. Pfaffenberger J. Szafranek M.G. Horning E.C. Horning 《Analytical biochemistry》1975,63(2):501-512
Thirty urinary collections from female and male neonates, juveniles, and adults have been analyzed by a new gas chromatographic (GC) method. Sixty meter high resolution open tubular glass capillary columns with a coating containing a dispersion of small particles of silanized silicic acid (Silanox) in SE-30 liquid phase were employed. Seventeen urinary polyols and aldoses were quantified through use of an internal reference compound and detector response factors. The components were analyzed as polyacetyl esters (from the polyols) and poly-O-acetylaldonic nitriles (from the aldoses). 相似文献
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Three prairie rattlesnakes (Crotalus viridis viridis) and two gopher snakes (Pituophis melanoleucus sayi) from the eastern high plains of New Mexico (USA) were examined for parasites. One cestode (Oochoristica osheroffi), and two nematode (Kalicephalus inermis and Physoloptera retusa) species were recovered from two infected rattlesnakes. One female gopher snake was infected with two nematode (K. inermis and Rhabdias spp.) and one mite (Entonyssus halli) species. 相似文献
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The mass spectra of some per-O-acetylaldono- and per-O-acetyldeoxyaldononitriles have been recorded. The major fragmentation-pathways are discussed in terms of the application of electron-impact mass-spectrometry to structural studies of aldoses. Both acetyl and deuterium-labelled acetyl derivatives are included. The spectra are useful in verifying the position of the deoxy group(s) of deoxyaldoses. 相似文献
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Hohensinner PJ Kaun C Rychli K Ben-Tal Cohen E Kastl SP Demyanets S Pfaffenberger S Speidl WS Rega G Ullrich R Maurer G Huber K Wojta J 《FEBS letters》2006,580(14):3532-3538
The chemokine MCP-1 is thought to play a key role - among many other pathophysiological processes - in myocardial infarction. MCP-1 is not only a key attractant for monocytes and macrophages and as such responsible for inflammation but might also be directly involved in the modulation of repair processes in the heart. We show that cultured human cardiac cells express MCP-1 and that its expression is upregulated by inflammatory cytokines and downregulated by hypoxia. We hypothesize that inflammation but not hypoxia is the main trigger for monocyte recruitment in the human heart. 相似文献
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Konstantin A. Krychtiuk Stefan P. Kastl Stefan Pfaffenberger Max Lenz Sebastian L. Hofbauer Anna Wonnerth Lorenz Koller Katharina M. Katsaros Thomas Pongratz Georg Goliasch Alexander Niessner Ludovit Gaspar Kurt Huber Gerald Maurer Elisabeth Dostal Johann Wojta Stanislav Oravec Walter S. Speidl 《PloS one》2015,10(4)
Objective
Atherosclerosis is considered to be an inflammatory disease in which monocytes and monocyte-derived macrophages play a key role. Circulating monocytes can be divided into three distinct subtypes, namely in classical monocytes (CM; CD14++CD16-), intermediate monocytes (IM; CD14++CD16+) and non-classical monocytes (NCM; CD14+CD16++). Low density lipoprotein particles are heterogeneous in size and density, with small, dense LDL (sdLDL) crucially implicated in atherogenesis. The aim of this study was to examine whether monocyte subsets are associated with sdLDL serum levels.Methods
We included 90 patients with angiographically documented stable coronary artery disease and determined monocyte subtypes by flow cytometry. sdLDL was measured by an electrophoresis method on polyacrylamide gel.Results
Patients with sdLDL levels in the highest tertile (sdLDL≥4mg/dL;T3) showed the highest levels of pro-inflammatory NCM (15.2±7% vs. 11.4±6% and 10.9±4%, respectively; p<0.01) when compared with patients in the middle (sdLDL=2-3mg/dL;T2) and lowest tertile (sdLDL=0-1mg/dL;T1). Furthermore, patients in the highest sdLDL tertile showed lower CM levels than patients in the middle and lowest tertile (79.2±8% vs. 83.9±7% and 82.7±5%; p<0.01 for T3 vs. T2+T1). Levels of IM were not related to sdLDL levels (5.6±4% vs. 4.6±3% vs. 6.4±3% for T3, T2 and T1, respectively). In contrast to monocyte subset distribution, levels of circulating pro- and anti-inflammatory markers were not associated with sdLDL levels.Conclusion
The atherogenic lipoprotein fraction sdLDL is associated with an increase of NCM and a decrease of CM. This could be a new link between lipid metabolism dysregulation, innate immunity and atherosclerosis. 相似文献9.
Fifty-two 24-hr urine collections from healthy male and female adults were analyzed by a new gas chromatographic method. High-resolution open tubular glass capillary columns with a coating of SE-30 liquid phase containing a dispersion of small particles of silanized silicic acid (Silanox) were employed. The components were analyzed as methoxime-trimethylsilyl ether derivatives. Seventeen urinary steroid metabolites were quantified through use of an internal reference compound. The results are interpreted as delineating two different types of steroid metabolism for premenopausal females: one group displays metabolic profiles closely resembling those of males. The biologic origin of this effect is not known, but it may be due to either late fetal or neonatal hormonal effects. 相似文献