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1.
Sugarcane plantlets were sectioned halfway between the base and the youngest ligule and then inoculated by soaking the wound in a suspension of Xanthomonas campestris pv. vasculorum. The infection caused rapid necrosis of the inoculated leaves, chlorosis of uninoculated leaves, or death of the inoculated plantlet. New tillers sometimes showed chlorosis or white streaks. The effects of the inoculum concentration, the cultivar, and the bacterial strain on symptom severity were determined. The ranking of cultivars depended on the inoculum concentration, and strains were found to differ with regard to aggressiveness. However, cultivars and strains were more effectively classified in greenhouse trials. The poor expression of leaf resistance appeared to limit the use of the in vitro test. 相似文献
2.
K Evangelou J Bramis I Peros P Zacharatos D Dasiou-Plakida N Kalogeropoulos 《Biotechnic & histochemistry》2013,88(1):5-10
It is well established that p16INK4A protein acts as a cell cycle inhibitor in the nucleus. Therefore, cytoplasmic localization of p16 INK4A usually is disregarded by investigators as nonspecific. Three recent studies reported findings that differ from the current view concerning p16INK4A immunohistochemical localization. All three demonstrated that breast and colon cancers expressing cytoplasmic p16INK4 represent distinct biological subsets. We previously detected in a percentage of non-small cell lung carcinomas simultaneous nuclear and cytoplasmic p16INK4A staining. In view of the reports concerning breast and colon carcinomas, we conducted an ultrastructural re-evaluation of our cases to clarify the specificity of p16INK4A cytoplasmic expression. We observed p16 INK4A immunolocalization in both the nucleus and the cytoplasm of a proportion of tumor cells. Diffuse dense nuclear staining was detected in the nucleoplasm, whereas weaker granular immunoreactivity was observed in the cytoplasm near the rough endoplasmic reticulum. Negative tumor cells also were visible. In the tumor-associated stromal, cells p16INK4A immunoreactivity was detected only in the nuclei. We have demonstrated that p16INK4A cytoplasmic staining is specific and suggest that it represents a mechanism of p16INK4A inactivation similar to that observed in other tumor suppressor genes. 相似文献
3.
N Papanikolaou G Peros P Morphake G Gkikas D Maraghianne G Tsipas K Kostopoulos C Arambatze E L Gkika J Bariety 《Prostaglandins, leukotrienes, and essential fatty acids》1992,45(2):131-136
Acute renal failure (ARF) induced with large doses of Gentamicin (GM) (an aminoglycoside) was associated with increased urinary TXB (TXA) excretion which provoked a decrease of the ratios of urinary PGE2/TXB2 and 6-keto-PGF1 alpha (PGI2)/TXB2 excretions. Furthermore, as indicated by light microscopy most of the epithelial cells lining the proximal tubules show obvious lesions varying from swelling of their cytoplasm to complete necrosis. Either the inhibitor, OKY-O46, of TXA-synthetase, or volume expansion (VE) with isotonic saline (IS) of the experimental animals diminished urinary TXB excretion which provoked 1) augmentation of the ratios of urinary PGE/TXB and 6-keto-PGF1 alpha/TXB excretions, 2) elevation of creatinine clearance (Ccr) and 3) diminution of proteinuria (PU). This protection against ARF-by OKY-O46 and VE can a can be seen in microscopic sections where necrosis of proximal tubules is almost absent. Only a few proximal tubules show swelling of their epithelial cells and some focal areas of tubule necrosis. We suggest that the metabolites of arachidonic acid (AA), TXA2 a (potent vasoconstrictor agent) and prostaglandins (PGE2 and PGI2), (potent vasodilator factors), play an important role in the development (TXA2) or in the prevention (PGs) of ARF induced by this antibiotic. 相似文献
4.
Variability among Vitis vinifera cultivars in micropropagation, organogenesis and antibiotic sensitivity 总被引:4,自引:0,他引:4
In vitro culture of 32 Vitis
vinifera cultivars and intraspecific hybrids was initiated from
axillary buds. The development of roots and shoots was followed during 14
subcultures on two hormone-free micropropagation media. One medium (M64)
was used until the 8th subculture, after which it was replaced by G90
medium which was found more suitable for plantlet growth and permitted an
increase in the time between subcultures. Large differences in plantlet
growth between cultivars were demonstrated on both media. The number of
roots had greatest variability between cultivars (CV=39%) as compared with
stem length (CV=21-22%) and number of nodes (CV=12-14%). The number of
nodes was positively correlated with shoot length whereas root number
appeared to be poorly positively correlated with shoot development.
Adventitious bud regeneration from leaves was studied for 20 cultivars and
averaged 36.7% of regenerative explants with large differences between
cultivars (CV=47%). However, organogenic competence was not correlated with
micropropagationability. High sensitivity of Vitis
vinifera plantlets to kanamycin and hygromycin was demonstrated
with a strong interaction between cultivar and antibiotic. At 0.8 mg
dm-3, hygromycin was lethal to plantlets. This
effect was only observed at 4 mg dm-3 for kanamycin,
whereas 1 mg dm-3 stimulated the development of
plantlets.Key words: Vitis vinifera, cultivar,
micropropagation, antibiotic, organogenesis.
相似文献
5.
6.
Synergistic interactions between interleukin 1, tumor necrosis factor, and lymphotoxin in bone resorption 总被引:20,自引:0,他引:20
P Stashenko F E Dewhirst W J Peros R L Kent J M Ago 《Journal of immunology (Baltimore, Md. : 1950)》1987,138(5):1464-1468
Cytokines with bone-resorbing activity include IL 1 beta (pI 7), IL 1 alpha (pI 5), tumor necrosis factor (TNF), and lymphotoxin (LT). Possible interaction between IL 1 beta, the major mediator with osteoclast-activating factor (OAF) activity, and other cytokines was studied. By itself, IL 1 beta was 13-fold more potent than IL 1 alpha and 1000-fold more potent than either TNF or LT in stimulating bone resorption. Suboptimal concentrations of IL 1 beta or IL 1 alpha in combination with suboptimal concentrations of TNF or LT resulted in synergistic bone-resorptive responses (1.5 to 10 times the expected responses if their effects were additive). Synergy between either form of IL 1 and TNF or LT resulted in a twofold increase in activity of IL 1, and a 100-fold increase in activity of TNF or LT. However, even with optimal synergy, IL 1 beta remained 20-fold more potent in inducing bone resorption than TNF or LT. Because IL 1 beta is considerably more potent than TNF and LT in stimulating bone resorption either alone or under synergistic conditions, it is unlikely that TNF and LT are responsible for more than a minor proportion of the total bone-resorbing activity formerly referred to as OAF. 相似文献
7.
Alexis Dereeper Stéphane Nicolas Loïc Le Cunff Roberto Bacilieri Agnès Doligez Jean-Pierre Peros Manuel Ruiz Patrice This 《BMC bioinformatics》2011,12(1):134
Background
High-throughput re-sequencing, new genotyping technologies and the availability of reference genomes allow the extensive characterization of Single Nucleotide Polymorphisms (SNPs) and insertion/deletion events (indels) in many plant species. The rapidly increasing amount of re-sequencing and genotyping data generated by large-scale genetic diversity projects requires the development of integrated bioinformatics tools able to efficiently manage, analyze, and combine these genetic data with genome structure and external data. 相似文献8.
9.
M. Stamataki D. Anninos E. Brountzos J. Georgoulakis J. Panayiotides Z. Christoni G. Peros P. Karakitsos 《Cytopathology》2008,19(1):11-18
Objective: This study investigates the role of liquid-based cytology by ThinPrep® technique in the detection of thyroid lesions.
Methods: In all, 252 specimens from 157 patients for pre-operative evaluation of thyroid nodules, prepared by the ThinPrep® , were examined. In all cases thyroidectomy followed the initial cytological evaluation. All cytological diagnoses were correlated to the histological ones.
Results: According to our findings, a sensitivity of 87.80%, a specificity of 99.50%, a positive predictive value of 97.30%, a negative predictive value of 97.56% and an overall accuracy of 97.52% were observed in fine needle aspiration cytology in correlation to the histological diagnosis after thyroidectomy.
Conclusions: ThinPrep® technique is a valid method for the pre-operative cytological diagnosis of thyroid nodules, offering the possibility of ancillary techniques, such as immunocytochemical and molecular methods and can, therefore, be potentially complementary to histological evaluation for further investigation of follicular lesions. 相似文献
Methods: In all, 252 specimens from 157 patients for pre-operative evaluation of thyroid nodules, prepared by the ThinPrep
Results: According to our findings, a sensitivity of 87.80%, a specificity of 99.50%, a positive predictive value of 97.30%, a negative predictive value of 97.56% and an overall accuracy of 97.52% were observed in fine needle aspiration cytology in correlation to the histological diagnosis after thyroidectomy.
Conclusions: ThinPrep
10.
K. Evangelou J. Bramis I. Peros P. Zacharatos D. Dasiou-Plakida N. Kalogeropoulos PJ Asimacopoulos C. Kittas E. Marinos VG Gorgoulis 《Biotechnic & histochemistry》2004,79(1):5-10
It is well established that p16INK4A protein acts as a cell cycle inhibitor in the nucleus. Therefore, cytoplasmic localization of p16 INK4A usually is disregarded by investigators as nonspecific. Three recent studies reported findings that differ from the current view concerning p16INK4A immunohistochemical localization. All three demonstrated that breast and colon cancers expressing cytoplasmic p16INK4 represent distinct biological subsets. We previously detected in a percentage of non-small cell lung carcinomas simultaneous nuclear and cytoplasmic p16INK4A staining. In view of the reports concerning breast and colon carcinomas, we conducted an ultrastructural re-evaluation of our cases to clarify the specificity of p16INK4A cytoplasmic expression. We observed p16 INK4A immunolocalization in both the nucleus and the cytoplasm of a proportion of tumor cells. Diffuse dense nuclear staining was detected in the nucleoplasm, whereas weaker granular immunoreactivity was observed in the cytoplasm near the rough endoplasmic reticulum. Negative tumor cells also were visible. In the tumor-associated stromal, cells p16INK4A immunoreactivity was detected only in the nuclei. We have demonstrated that p16INK4A cytoplasmic staining is specific and suggest that it represents a mechanism of p16INK4A inactivation similar to that observed in other tumor suppressor genes. 相似文献