全文获取类型
收费全文 | 1862篇 |
免费 | 242篇 |
国内免费 | 1篇 |
专业分类
2105篇 |
出版年
2023年 | 8篇 |
2022年 | 23篇 |
2021年 | 35篇 |
2020年 | 30篇 |
2019年 | 45篇 |
2018年 | 39篇 |
2017年 | 51篇 |
2016年 | 66篇 |
2015年 | 88篇 |
2014年 | 108篇 |
2013年 | 116篇 |
2012年 | 116篇 |
2011年 | 117篇 |
2010年 | 98篇 |
2009年 | 73篇 |
2008年 | 93篇 |
2007年 | 99篇 |
2006年 | 70篇 |
2005年 | 81篇 |
2004年 | 51篇 |
2003年 | 73篇 |
2002年 | 58篇 |
2001年 | 56篇 |
2000年 | 62篇 |
1999年 | 54篇 |
1998年 | 22篇 |
1997年 | 16篇 |
1996年 | 24篇 |
1995年 | 9篇 |
1994年 | 15篇 |
1993年 | 18篇 |
1992年 | 26篇 |
1991年 | 24篇 |
1990年 | 22篇 |
1989年 | 26篇 |
1988年 | 15篇 |
1987年 | 26篇 |
1986年 | 24篇 |
1985年 | 17篇 |
1983年 | 12篇 |
1982年 | 5篇 |
1981年 | 6篇 |
1980年 | 7篇 |
1979年 | 9篇 |
1978年 | 8篇 |
1977年 | 8篇 |
1976年 | 6篇 |
1975年 | 7篇 |
1974年 | 7篇 |
1973年 | 7篇 |
排序方式: 共有2105条查询结果,搜索用时 0 毫秒
1.
Fiddler crabs (Uca spp., Decapoda: Ocypodidae) are commonly found forming large aggregations in intertidal zones, where they perform rhythmic waving displays with their greatly enlarged claws. While performing these displays, fiddler crabs often synchronize their behavior with neighboring males, forming the only known synchronized visual courtship displays involving reflected light and moving body parts. Despite being one of the most conspicuous aspects of fiddler crab behavior, little is known about the mechanisms underlying synchronization of male displays. In this study we develop a spatially explicit model of fiddler crab waving displays using coupled logistic map equations. We explored two alternative models in which males either direct their attention at random angles or preferentially toward neighbors. Our results indicate that synchronization is possible over a fairly large region of parameter space. Moreover, our model was capable of generating local synchronization neighborhoods, as commonly observed in fiddler crabs under natural conditions. 相似文献
2.
Victor F. Medina Peter M. Jeffers Steven L. Larson Waleska Perez 《International journal of phytoremediation》2000,2(4):287-295
Bleach treatment of plants was studied as a simple alternative to axenic tissue cultures for demonstrating phytodegradation of aqueous and gas-phase environmental contaminants. Parrotfeather (Myriophyllum aquaticum), spinach (Spinacia oleracea), and wheat (Triticum aestivum) were exposed to 0.525% NaC10 solutions for 15 s, then rinsed in deionized water. Plate counts indicated that 97 to 100% of viable bacteria were removed from parrotfeather and spinach. Transformation rates for 2,4,6-trinitrotoluene (TNT) by bleached and untreated parrotfeather were virtually identical. Similarly, treated and untreated spinach, wheat heads, and wheat leaves removed methyl bromide (MeBr) from air at the same rates. However, wheat root with attendant adhering soil was rendered inactive by bleach treatment. Parrotfeather roots examined by dissecting microscope and by electron microscope showed no significant damage caused by bleach treatment. 相似文献
3.
4.
P. Cruz C. H. Mejia‐Ruiz R. Perez‐Enriquez A. M. Ibarra 《Molecular ecology resources》2002,2(3):239-241
Five polymorphic microsatellite loci were characterized for Penaeus (Litopenaeus) vannamei. Loci were isolated using a partial Sau3A1 genomic library by the sequencing of randomly selected clones and by a biotinylated (CT)10 and (GT)10 probes screening procedure. The last strategy resulted in the most useful data. About 40% of the clones showed a previously reported satellite/microsatellite (PVS1), reducing the chance of finding new microsatellite regions. Whereas two of the microsatellite loci with more than 10 alleles will be useful for mating analysis in a breeding program, the others might prove useful for population genetic studies. 相似文献
5.
M Mourelle V M Perez M Rojkind 《The journal of histochemistry and cytochemistry》1988,36(12):1471-1474
We describe a simple, sensitive, and quantitative procedure for measurement of triglycerides and protein contents in formalin-fixed liver sections. The method can detect as little as 0.27 microgram of triglycerides per mg of protein. It is based on selective binding of Sudan IV and Fast Green FCF to fat and total proteins, respectively, and their sequential elution with solvents. Sudan IV is eluted readily with acetone and Fast Green with NaOH-methanol, and the absorbances obtained at 500 and 610 nm can be used to determine the amount of triglycerides and total protein. The color equivalence for Fast Green was obtained after destaining the sections and measuring the protein contents by micro-Kjeldahl analysis. The color equivalence of Sudan IV was estimated by determining the triglyceride content in liver homogenates by an enzymatic procedure and then measuring the amount of dye bound to multiple fixed sections. There was a strong linear correlation between the triglyceride content as determined chemically and that obtained using the equivalence colors (r2 = 0.98). This method is useful to measure fat content in tissue samples and could be applied to evaluate the progression of liver disease. 相似文献
6.
Targeted cytotoxic cells in human peripheral blood lymphocytes. 总被引:2,自引:0,他引:2
M A Garrido P Perez J A Titus M J Valdayo D F Winkler S A Barbieri J R Wunderlich D M Segal 《Journal of immunology (Baltimore, Md. : 1950)》1990,144(8):2891-2898
We have isolated subsets of cells from human PBL and have investigated their abilities to mediate lysis targeted by bispecific antibodies. Targeted cytotoxic cells were divided into two distinct types based on buoyant density. The low buoyant density fraction contained all of the targetable cytotoxic activity in unstimulated PBL, including both T and K cells targeted with anti-CD3 and anti-Fc gamma RIII (CD16) containing bispecific antibodies, respectively. Both types of targetable cytotoxic cells required IL-2 for maintenance of cytotoxic activity, expressed the CD56 (NKH1) marker, and mediated MHC-unrestricted lysis. The targetable T cells in low density PBL were exclusively CD8+ and represented only about 2% of the total PBL. The high buoyant density lymphocytes, depleted of NK cells, had no targetable activity, but were able to generate over several days, targetable T cell activity in the presence of a TCR cross-linking signal plus IL-2. Unlike the low-density cells, the activated high buoyant density effector T cells did not express CD56, consisted of both CD4+ and CD8+ cells, and did not mediate MHC-unrestricted lysis. These cells proliferated more rapidly and generated more total lytic activity than the low-density fraction. Our studies show that targetable cytotoxic activity in human PBL is mediated by several subsets of cells with different activation requirements. Presumably all of these activities could be directed against unwanted cells in clinical or preclinical studies involving targeted cytotoxic cells. 相似文献
7.
8.
M D Perez L Sanchez P Aranda J M Ena R Oria M Calvo 《Biochimica et biophysica acta》1992,1123(2):151-155
The interaction of bovine beta-lactoglobulin with palmitic and oleic acids has been studied by a partition equilibrium method. Bovine beta-lactoglobulin displays only one high affinity binding site for fatty acids whose association constants for palmitic and oleic acids are 4.2 x 10(6) and 2.3 x 10(6) M-1, respectively. However, other binding sites with low affinity are also present. The existence of one high affinity binding site is in accordance with the amount of fatty acids naturally bound to beta-lactoglobulin isolated from milk. The effect of beta-lactoglobulin on ruminant pregastric lipases from a pharyngeal extract has been assayed. The activity of pharyngeal lipase on a triglyceride emulsion is increased about 200%, 250% and 190% in the presence of 10 mg/ml, 20 mg/ml and 40 mg/ml of beta-lactoglobulin, respectively, the last concentration representing that found physiologically in colostrum. Albumin, another ligand-binding protein, increases the activity of this enzyme to a lesser extent and high levels tend to inhibit enzyme action. These results indicate that beta-lactoglobulin could participate in the digestion of milk lipids during the neonatal period by enhancing the activity of pregastric lipase through removal of the fatty acids that inhibit this enzyme. 相似文献
9.
10.
M. A. Perez-Parron M. E. Gonzalez-Benito C. Perez 《Plant Cell, Tissue and Organ Culture》1994,37(3):297-302
Micropropagation of Fraxinus angustifolia Vahl has been successfully achieved both from mature and juvenile plant material using shoot tip and nodal explants. Several basal media supplemented with benzyladenine (BA) and indolebutyric acid (IBA) were tested for shoot proliferation. The most new explants per mature explant (5.3) was obtained on DKW medium plus 4.4 M BA+0.98 M IBA. The most new explants per juvenile explant (5.6) was produced on QL medium plus 8.9 M BA+0.49 M IBA. Rooting was achieved on WPM supplemented with 0.98–4.9 M IBA. Rooted plantlets were transferred to soil and acclimatized with 85% survival.Abbreviations BA
benzyladenine
- IBA
indolebutyric acid
- NAA
1-naphthaleneacetic acid 相似文献