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1.
Genetic control of PI and GC variants in the American Mink 总被引:1,自引:0,他引:1
Genetic polymorphism of the serum α-protease inhibitor (PI) and group-specific component (GC) in minks was revealed using one-dimensional polyacrylamide gel electrophoresis and immunoblotting. Two codominant alleles were identified at each of the two loci. The data ruled out the possibility of any linkage between the PI, GC and the coat colour gene Crystal ( Cr ). 相似文献
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Multiple 2′-5′ oligoadenylate (2-5A) synthetases are important components of innate immunity in mammals. Gene families encoding
these proteins have previously been studied mainly in humans and mice. To reconstruct the evolution of this gene family in
mammals, a search for additional 2-5A synthetase genes was performed in rat, cattle, pig, and dog. Twelve 2′-5′ oligoadenylate
synthetase (Oas) genes were identified in the rat genome, including eight Oas1 genes, two Oas1 pseudogenes, single copies
of Oas2 and Oas3, and two Oas-like genes, Oasl1 and Oasl2. Four OAS genes were detected in the pig genome and five OAS genes
were found in both the cattle and dog genomes. An OAS3 gene was not found in either the cattle or the pig genome. While two
tandemly duplicated OAS-like (OASL) genes were identified in the dog genome, only a single OASL orthologue was found in both
the cattle and the pig genomes. The bovine and porcine OASL genes contain premature stop codons and encode truncated proteins,
which lack the typical C-terminal double ubiquitin domains. The cDNA sequences of the rat, cattle, pig, and dog OAS genes
were amplified, sequenced and compared with each other and with those in the human, mouse, horse, and chicken genomes. Evidence
of concerted evolution of paralogous 2′-5′ oligoadenylate synthetase 1 genes was obtained in rodents (Rodentia) and even-toed
ungulates (Artiodactyla). Calculations using the nonparametric Kolmogorov-Smirnov test suggested that the homogenization of
paralogous OAS1 sequences was due to gene conversion rather than stabilizing selection.
Electronic Supplementary Material Electronic Supplementary material is available for this article at
and accessible for authorised users.
Reviewing Editor: Dr. Martin Kreitman 相似文献
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E. V. Mitsevich I. P. Mitsevich V. V. Perelygin Do Ngok Lan' Nguen Thu Hoai 《Applied Biochemistry and Microbiology》2000,36(6):582-588
The diversity of microorganisms from soils treated in the past with various dosages of dioxin-containing defoliants was studied. Population alterations dependent on dioxin concentrations were elucidated. Soil fungi and, to a smaller extent, actinomycetes were found to be the most sensitive to dioxins. 相似文献
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A. A. Perelygin P. B. Samollow L. M. Perelygina L. M. Cherry S. M. Mahaney J. L. VandeBerg 《Animal genetics》1996,27(2):113-116
A 323-bp DNA fragment (U15557) was isolated, cloned, and sequenced after polymerase chain reaction (PCR) amplification from Monodelphis domestica genomic DNA. AHindIII restriction fragment length polymorphism was identified in this species using the U15557 PCR. fragment as a hybridization probe. DNA samples exhibited either a 6.4kb band, a 7.2 kb band, or both bands simultaneously. Behaviour of these two variants in family studies was consistent with codominant autosomal inheritance. Linkage between this marker and the loci encoding protease inhibitor (PI) and adenylate kinase 1 (AK1) was found in M. domestica. 相似文献
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Aruna Kasoju M Lakshmi Narasu Charuvaka Muvva Bathula VV SubbaRao 《Bioinformation》2012,8(14):684-686
Aflatoxins are polyketide-derived secondary metabolites produced by Aspergillus spp. The toxic effects of aflatoxins have adverse
consequences for human health and agricultural economics. The aflR gene, a regulatory gene for aflatoxin biosynthesis, encodes a
protein containing a zinc-finger DNA-binding motif. AFLR-Protein three-dimensional model was generated using Robetta server.
The modeled AFLR-Protein was further optimization and validation using Rampage. In the simulations, we monitored the
backbone atoms and the C-α-helix of the modeled protein. The low RMSD and the simulation time indicate that, as expected, the
3D structural model of AFLR-protein represents a stable folding conformation. This study paves the way for generating computer
molecular models for proteins whose crystal structures are not available and which would aid in detailed molecular mechanism of
inhibition of aflatoxin. 相似文献
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Natalia M. Astakhova Andrey A. Perelygin Andrey A. Zharkikh Teri L. Lear Stephen J. Coleman James N. MacLeod Margo A. Brinton 《Immunogenetics》2009,61(7):529-539
Toll-like receptors 3, 7, and 8 (TLR3, TLR7, and TLR8) were studied in the genomes of the domestic horse and several other
mammals. The messenger RNA sequences and exon/intron structures of these TLR genes were determined. An equine bacterial artificial
chromosome clone containing the TLR3 gene was assigned by fluorescent in situ hybridization to the horse chromosomal location
ECA27q16–q17 and this map location was confirmed using an equine radiation hybrid panel. Direct sequencing revealed 13 single-nucleotide
polymorphisms in the coding regions of the equine TLR 3, 7, and 8 genes. Of these polymorphisms, 12 were not previously reported.
The allelic frequency was estimated for each single-nucleotide polymorphism from genotyping data obtained for 154 animals
from five horse breeds. Some of these frequencies varied significantly among different horse breeds. Domain architecture predictions
for the three equine TLR protein sequences revealed several conserved regions within the variable leucine-rich repeats between
the corresponding horse and cattle TLR proteins. A phylogenetic analysis did not indicate that any significant exchanges had
occurred between paralogous TLR7 and TLR8 genes in 20 vertebrate species analyzed.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
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E. A. Svetoch B. V. Eruslanov Y. N. Kovalev E. V. Mitsevich I. P. Mitsevich V. P. Levchuk N. K. Fursova V. V. Perelygin Y. G. Stepanshin M. G. Teymurasov B. S. Seal N. J. Stern 《Probiotics and antimicrobial proteins》2009,1(2):136-142
The antimicrobial spectra of previously published bacteriocin E 50–52 (39 a.a.; 3,932 Da; pI = 8.5) and bacteriocin B 602 (29 a.a.; 3,864 Da; pI = 7.2) were determined. Named peptides were related to class IIa (pediocin-like) bacteriocins. Minimal inhibitory concentrations (MICs) of bacteriocins have been determined for bacterial isolates that were causative agents of nosocomial infections collected from Russian hospitals in 2003–2007, namely methicillin-resistant Staphylococcus aureus (MRSA) (n = 10); Acinetobacter baumannii (n = 11); Citrobacter freundii (n = 8); Escherichia coli (n = 9); Klebsiella pneumoniae (n = 10); Proteus spp. (n = 6); and Pseudomonas aeruginosa (n = 10). The majority of these tested isolates have been shown to be multidrug resistant and carry genetic determinants of antimicrobial resistance that were detected using polymerase chain reaction (PCR). The MICs of bacteriocin B 602 ranged from ≤0.025–1.56 μg/ml, and for bacteriocin E 50–52 from 0.05 to 6.25 μg/ml for all of 64 bacterial clinical isolates tested. Interestingly, the bacteriocins studied demonstrate activity on both Gram-positive and Gram-negative bacteria. Bacteriocins E 50–52 and B 602 show good activity against nosocomial bacterial agents resistant to many classes of modern antibacterials used in clinical practice. These bacteriocins should be examined as an alternative in treating infections caused by such agents. 相似文献
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Mammalian 2'-5' oligoadenylate (2-5A) synthetases are important mediators of the antiviral activity of interferons. Both human and mouse 2-5A synthetase gene families encode four forms of enzymes: small, medium, large and ubiquitin-like. In this study, the structures of four equine OAS genes were determined using DNA sequences derived from fifteen cDNA and four BAC clones. Composition of the equine OAS gene family is more similar to that of the human OAS family than the mouse Oas family. Two OAS-containing bovine BAC clones were identified in GenBank. Both equine and bovine BAC clones were physically assigned by FISH to horse and cattle chromosomes, ECA8p15-->p14 and BTA17q24--> q25, respectively. The comparative mapping data confirm conservation of synteny between ungulates, humans and rodents. 相似文献
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