排序方式: 共有37条查询结果,搜索用时 15 毫秒
1.
Burkart V Kim YE Hartmann B Ghiea I Syldath U Kauer M Fingberg W Hanifi-Moghaddam P Müller S Kolb H 《Journal of immunology (Baltimore, Md. : 1950)》2002,168(4):1730-1737
The cholera toxin B chain (CTB) has been reported to suppress T cell-dependent autoimmune diseases and to potentiate tolerance of the adaptive immune system. We have analyzed the effects of CTB on macrophages in vitro and have found that preincubation with CTB (10 microg/ml) suppresses the proinflammatory reaction to LPS challenge, as demonstrated by suppressed production of TNF-alpha, IL-6, IL-12(p70), and NO (p < 0.01) in cells of macrophage lines. Pre-exposure to CTB also suppresses LPS-induced TNF-alpha and IL-12(p70) formation in human PBMC. Both native and recombinant CTB exhibited suppressive activity, which was shared by intact cholera toxin. In cells of the human monocyte line Mono Mac 6, exposure to CTB failed to suppress the production of IL-10 in response to LPS. Control experiments excluded a role of possible contamination of CTB by endotoxin or intact cholera toxin. The suppression of TNF-alpha production occurred at the level of mRNA formation. Tolerance induction by CTB was dose and time dependent. The suppression of TNF-alpha and IL-6 production could be counteracted by the addition of Abs to IL-10 and TGF-beta. IFN-gamma also antagonized the actions of CTB on macrophages. In contrast to desensitization by low doses of LPS, tolerance induction by CTB occurred silently, i.e., in the absence of a measurable proinflammatory response. These findings identify immune-deviating properties of CTB at the level of innate immune cells and may be relevant to the use of CTB in modulating immune-mediated diseases. 相似文献
2.
Rocio Acuna-Hidalgo Denny Schanze Ariana Kariminejad Ann Nordgren Mohamad Hasan Kariminejad Peter Conner Giedre Grigelioniene Daniel Nilsson Magnus Nordenskjöld Anna Wedell Christoph Freyer Anna Wredenberg Dagmar Wieczorek Gabriele Gillessen-Kaesbach Hülya Kayserili Nursel Elcioglu Siavash Ghaderi-Sohi Payman Goodarzi Hamidreza Setayesh Maartje van de Vorst Marloes Steehouwer Rolph Pfundt Birgit Krabichler Cynthia Curry Malcolm G. MacKenzie Kym M. Boycott Christian Gilissen Andreas R. Janecke Alexander Hoischen Martin Zenker 《American journal of human genetics》2014
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Najmabadi H Motazacker MM Garshasbi M Kahrizi K Tzschach A Chen W Behjati F Hadavi V Nieh SE Abedini SS Vazifehmand R Firouzabadi SG Jamali P Falah M Seifati SM Grüters A Lenzner S Jensen LR Rüschendorf F Kuss AW Ropers HH 《Human genetics》2007,121(1):43-48
Autosomal recessive gene defects are arguably the most important, but least studied genetic causes of severe cognitive dysfunction.
Homozygosity mapping in 78 consanguineous Iranian families with nonsyndromic autosomal recessive mental retardation (NS-ARMR)
has enabled us to determine the chromosomal localization of at least 8 novel gene loci for this condition. Our data suggest
that in the Iranian population NS-ARMR is very heterogeneous, and they argue against the existence of frequent gene defects
that account for more than a few percent of the cases.
Mohammad Mahdi Motazacker and Masoud Garshasbi have contributed equally to this work. 相似文献
5.
Ebrahim Karimi Payman Abbaszadeh Dahaji Yadola Dalvand Mahtab Omidvari Mozhdeh Kakuei Nezhad 《Biocontrol Science and Technology》2012,22(3):333-349
Biological control of fungi causing root rot on sugar beet by native Streptomyces isolates (C and S2) was evaluated in this study. The dry weight and colony forming unit (CFU) of S2 and C increased when 300 mM NaCl was added to medium. The in vitro antagonism assays showed that both isolates had inhibitory effect against Rhizoctonia solani AG-2, Fusarium solani and Phytophthora drechsleri. In dual culture, Streptomyces isolate C inhibited mycelial growth of R. solani, F. solani and P. drechsleri 45%, 53% and 26%, respectively. NaCl treatment of medium increased biocontrol activity of soluble and volatile compounds of isolate C and S2. After salt treatment, growth inhibition of R. solani, F. solani and P. drechsleri by isolate C increased up to 59%, 70% and 79%, respectively. To elucidate the mode of antagonism, protease, chitinase, beta glucanase, cellulase, lipase and α-amylase activity and siderophore and salicylic acid (SA) production were evaluated. Both isolates showed protease, chitinase and α-amylase activity. Also, biosynthesis of siderophore was detectable for both isolates. Production of siderophore and activity of protease and α-amylase increased after adding salt for both isolates. In contrast, chitinase activity decreased significantly. Production of SA, beta glucanase and lipase by isolate S2 and biosynthesis of cellulase by isolate C were observed in presence and absence of NaCl. Soil treatment with Streptomyces isolate C inhibited root rot of sugar beet caused by P. drechsleri, R. solani and F. solani. Results of this study showed that these two Streptomyces isolates had potential to be utilized as biocontrol agent against fungal diseases especially in saline soils. 相似文献
6.
Taati M Moghadasi M Dezfoulian O Asadian P Kheradmand A Abbasi M Zendehdel M 《Journal of physiology and biochemistry》2012,68(1):91-97
Ghrelin, the endogenous ligand for growth hormone secretagogue receptor, has been reported to prevent ischemia/reperfusion
(I/R) injury in various tissues by its antioxidant activity. Therefore, this study was aimed to investigate the effect of
ghrelin on sperm quality and antioxidant enzyme activity in a rat testicular ischemia/reperfusion injury model. Forty-two
male Wistar rats were divided into groups control, I/R, and I/R plus ghrelin. The right testes were rotated 720° for 1 h and
were allowed to reperfuse for 4 h and 30 days thereafter. Ghrelin (40 nmol/kg IP) or vehicle (physiological saline) was administrated
15 min before reperfusion. After 4 h of reperfusion, a right orchiectomy was performed to measure the biochemical parameters.
In addition, the sperm was collected from the epididymis after 30 days of reperfusion, and sperm characteristics were examined.
The malondialdehyde levels of the testis tissues were significantly increased, but a statistically significant decrease was
found in the superoxide dismutase, glutathione peroxidase, and catalase activities in the I/R group as compared with the control,
indicating I/R injury. The sperm evaluation showed a significant reduction in all characteristics resulted from I/R compared
with the control. In the ghrelin-treated group, the malondialdehyde values were significantly lowered, and only enzyme activity
of glutathione peroxidase showed significant increases compared with the I/R group. Ghrelin significantly enhanced sperm motility,
movement, and concentration but did not prevent I/R-induced reduction in membrane integrity in the testes of rats compared
to the I/R group. Our results suggest that ghrelin treatment has a protective role on IR-induced testicular injury, and this
effect may be due to its antioxidant properties. 相似文献
7.
Jennifer F. Knight Vanessa Y.C. Sung Elena Kuzmin Amber L. Couzens Danielle A. de Verteuil Colin D.H. Ratcliffe Paula P. Coelho Radia M. Johnson Payman Samavarchi-Tehrani Tina Gruosso Harvey W. Smith Wontae Lee Sadiq M. Saleh Dongmei Zuo Hong Zhao Marie-Christine Guiot Ryan R. Davis Jeffrey P. Gregg Morag Park 《Cell reports》2018,22(12):3191-3205
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A Mostafazadeh C Herder B Haastert P Hanifi-Moghaddam N Schloot W Koenig T Illig B Thorand R Holle M-B Eslami H Kolb 《Hormones et métabolisme》2005,37(4):257-263
The relationship between humoral immunity to hsp60 and type 2 diabetes along with other relevant metabolic, inflammatory and immunogenetic variables was studied in 76 non-diabetic and 74 diabetic persons aged 55-74 years selected from the population-based KORA Survey 2000. Antibodies to human hsp60 were measured in serum samples by ELISA. Hsp60 antibodies were detected in all but two individuals in a considerable range of titres (22-1,856 AU/ml). There was no significant association to age and sex, or to key clinical or metabolic parameters (BMI, WHR, HbA1c, total cholesterol, LDL cholesterol, HDL cholesterol, systolic and diastolic blood pressure, albumin, uric acid) or immunological parameters (CRP, IL-6, sIL-6R, TNFalpha, sTNFalpha R60, sTNFalpha R80). Analysis of antibody-positive individuals revealed an association between hsp60 antibodies and diabetes at borderline significance (p = 0.047), which was lost when the two antibody-negative individuals were included. Genetic analyses indicated that this association was significant in carriers of the C allele of the IL-6 promoter region polymorphism at nucleotide -174 (p = 0.02), but not in GG genotype carriers. We conclude that humoral immunity to human hsp60 may be enhanced in those diabetic patients carrying the -174C allele of the IL-6 gene. This finding may contribute to an understanding of the relationship between the -174C allele and increased risk of atherosclerosis. 相似文献
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There is accumulating evidence that excitotoxicity and oxidative stress resulting from excessive activation of glutamate (N-methyl-d-aspartate) NMDA receptors are major participants in striatal degeneration associated with 3-nitropropionic acid (3NP) administration.
Although excitotoxic and oxidative mechanisms are implicated in 3NP toxicity, there are conflicting reports as to whether
NMDA receptor antagonists attenuate or exacerbate the 3NP-induced neurodegeneration. In the present study, we investigated
the involvement of NMDA receptors in striatal degeneration, protein oxidation and motor impairment following systemic 3NP
administration. We examined whether NMDA receptor antagonists, memantine and ifenprodil, influence the neurotoxicity of 3NP.
The development of striatal lesion and protein oxidation following 3NP administration is delayed by memantine but not affected
by ifenprodil. However, in behavioral experiments, memantine failed to improve and ifenprodil exacerbated the motor deficits
associated with 3NP toxicity. Together, these findings suggest caution in the application of NMDA receptor antagonists as
a neuroprotective agent in neurodegenerative disorders associated with metabolic impairment. 相似文献