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1.
Jér?me Trzcionka Arielle Noirot Paul-Louis Fabre Nadia Chouini-Lalanne 《Photochemical & photobiological sciences》2005,4(3):298-303
The photophysical properties of indoprofen photoproducts have been examined in various solvents by absorbance and emission spectroscopies in relation with their photosensitizing properties. The photophysical properties of 2-[4-(1-hydroxy)ethylphenyl]isoindolin-1-one (HOINP) and 2-(4-ethylphenyl)isoindolin-1-one (ETINP) are typical of a singlet excited state when the ones of 2-(4-acetylphenyl)isoindolin-1-one (KINP) are based on its triplet excited state according to previous work. The effect of solvent polarity on the absorption and fluorescence properties of HOINP and ETINP has been investigated as a function of Delta f, the Lippert solvent polarity parameter. A solvatochromic effect, function of the polarity region, has been observed for both photoproducts due to a change in the dipole moment of the compound upon excitation. In low-polarity regions, the excited state dipole moment of HOINP undergoes only a moderate increase (11.5 D) as compared to the dipole moment of the ground state (4.5 D) suggesting that the fluorescence arises from the locally excited state while in high-polarity regions it is strongly increased (42.9 D), which can imply that the emission takes place from a charge transfer state. In the case of ETINP, it would seem that the emitting state is rather a charge transfer state whatever the region is (16.9 and 31.8 D for the calculated excited-state dipole moments in the low and high-polarity regions, respectively). HOINP and ETINP do not produce thymine dimers by photosensitization but induce photooxidative damage via an electron transfer mechanism. 相似文献
2.
Delcourt Alain Mathieu Gisèle Baji Halima Kimny Tan Flammang Michel Compagnon Paul-Louis 《Mycopathologia》1997,137(1):27-32
Six new polyazole derivatives from 2-(2,4-dichlorophenyl)-1,3-dioxolane were studied for their antifungal activity against pathogenic fungi for humans and animals. The antimycotic effect was largely restricted to the filamentous fungi with Aspergillus fumigatus and Scedosporium apiospermum being remarkably susceptible. Three compounds were as effective as the two references, ketoconazole and oxiconazole. The structure-activity relationships revealed that an oxime group combined with four chlorine atoms increased the antifungal properties of the chemical series.This revised version was published online in October 2005 with corrections to the Cover Date. 相似文献
3.
Summary A mutant of Escherichia coli K-12 was isolated as conditionally deficient in the expression of two exported proteins simultaneously (i.e. two acid phosphatases) The mutant was found to be thermosensitive on minimal medium at 37°C and above, but grew normally on rich media at these temperatures. The mutation, named expA and located at 22 min on the recalibrated linkage map, depressed the levels of six periplasmic enzymatic activities in bacteria grown at 37°C. At least ten proteins were greatly reduced in the periplasm under these conditions. The mutation also affected some outer membrane proteins, among which were the ompF protein and a protein which may be protein III, but had little effect on cytoplasmic membrane proteins. The gel patterns of the soluble cytoplasmic proteins were not modified except for one major protein of MW 47,000. The activities of -galactosidase and of aspartate transcarbamylase were unmodified. After growth at 30°C no difference was observed between expA and expA
+ isogenic strains. The results are discussed with respect to the mechanism of protein export.Enzymes E.C. 3.1.3.2
Acid phosphatase (optimum pH 2.5)
- E.C. 3.1.3.2
Acid phosphatase (optimum pH 4.5)
- E.C. 3.1.3.1
Alkaline phosphatase
- E.C. 3.4.11
Aminopeptidase-N
- E.C. 2.1.3.2
Aspartate transcarbamylase
- E.C. 3.2.1.23
-galactosidase
- E.C. 3.1.27.1
RNase I
Abbreviations PNP-OH
Para-nitro-phenol
- PNA
Para-nitro-anilide
- PNP-P
Para-nitro-phenyl-phosphate
- Bis-PNP-P
Bis-para-nitro-phenyl-phosphate
- IPTG
Isopropyl--thiogalactopyranoside, Nitrosoguanidine N-methyl, N'-nitro, N, Nitrosoguanidine, Tris Tris (hydromethyl) aminomethane 相似文献
4.
Nicolas Coant Sanae Ben Mkaddem Eric Pedruzzi Cécile Guichard Xavier Tréton Robert Ducroc Jean-Noel Freund Dominique Cazals-Hatem Yoram Bouhnik Paul-Louis Woerther David Skurnik Alain Grodet Michèle Fay Denis Biard Thécla Lesuffleur Christine Deffert Richard Moreau André Groyer Karl-Heinz Krause Fanny Daniel Eric Ogier-Denis 《Molecular and cellular biology》2010,30(11):2636-2650
5.
Christophe Rodriguez Nicolas de Prost Slim Fourati Claudie Lamoureux Guillaume Gricourt Melissa Ndebi Florence Canoui-Poitrine Isaac Dsveaux Oriane Picard Vanessa Demontant Elisabeth Trawinski Raphaël Lepeule Laure Surgers William Vindrios Jean-Daniel Lelivre Nicolas Mongardon Olivier Langeron Jos L. Cohen Armand Mekontso-Dessap Paul-Louis Woerther Jean-Michel Pawlotsky 《PLoS pathogens》2021,17(3)
6.
Xavier Tréton Eric Pedruzzi Cécile Guichard Yannick Ladeiro Shirin Sedghi Mélissa Vallée Neike Fernandez Emilie Bruyère Paul-Louis Woerther Robert Ducroc Nicolas Montcuquet Jean-Noel Freund Isabelle Van Seuningen Frédérick Barreau Assiya Marah Jean-Pierre Hugot Dominique Cazals-Hatem Yoram Bouhnik Fanny Daniel Eric Ogier-Denis 《PloS one》2014,9(7)
Ulcerative colitis (UC) is a chronic inflammatory bowel disease affecting the rectum which progressively extents. Its etiology remains unknown and the number of treatments available is limited. Studies of UC patients have identified an unbalanced endoplasmic reticulum (ER) stress in the non-inflamed colonic mucosa. Animal models with impaired ER stress are sensitive to intestinal inflammation, suggesting that an unbalanced ER stress could cause inflammation. However, there are no ER stress-regulating strategies proposed in the management of UC partly because of the lack of relevant preclinical model mimicking the disease. Here we generated the IL10/Nox1dKO mouse model which combines immune dysfunction (IL-10 deficiency) and abnormal epithelium (NADPH oxidase 1 (Nox1) deficiency) and spontaneously develops a UC-like phenotype with similar complications (colorectal cancer) than UC. Our data identified an unanticipated combined role of IL10 and Nox1 in the fine-tuning of ER stress responses in goblet cells. As in humans, the ER stress was unbalanced in mice with decreased eIF2α phosphorylation preceding inflammation. In IL10/Nox1dKO mice, salubrinal preserved eIF2α phosphorylation through inhibition of the regulatory subunit of the protein phosphatase 1 PP1R15A/GADD34 and prevented colitis. Thus, this new experimental model highlighted the central role of epithelial ER stress abnormalities in the development of colitis and defined the defective eIF2α pathway as a key pathophysiological target for UC. Therefore, specific regulators able to restore the defective eIF2α pathway could lead to the molecular remission needed to treat UC. 相似文献
7.
Vieillard J Mazurczyk R Morin C Hannes B Chevolot Y Desbène PL Krawczyk S 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2007,845(2):218-225
This paper describes the fabrication, the characterization and the applications of a capillary electrophoresis microchip. This hybrid device (glass/PDMS) features channels and optical waveguides integrated in one common substrate. It can be used for electrophoretic separation and fluorimetric detection of molecules. The microfluidic performance of the device is demonstrated by capillary zone and gel electrophoresis of proteins. 相似文献
8.
Reaction of excess CNCH2SiMe3(L) with CuX2·nH2O (X=NO3−, n=3; ClO4−, n=6) in THF gives the CuI complexes [CuL4]NO3 (1) and [CuL4]ClO4 (2). When CuCN is used as starting material, complex 3, Cu(L3)CN, C4H10O·3H2O, is obtained. Immediate reduction occurs with AgNO3 precipitating metallic Ag. Reactions with MnCl2·6H2O and Mn(NO3)2·6H2O in THF produce two new compounds which analyze as MnL4Cl2·4H2O (6) and MnL2(NO3)2·H2O (7). When excess p-tolylsulfonylmethylisocyanide (L′) is reacted with Cu(NO3)2, the mixed-valence CuI---CuII complex Cu2L′6(NO3)3 (5) is precipitated, while using CuCN gives the CuI dimer Cu2L′4(CN)2 (4). In analogous conditions the manganese complex MnL′2(NO3)2·C3H6O·3H2O (8) is precipitated. All these complexes have been isolated, characterized by IR, NMR for diamagnetic species, magnetic susceptibilities, EPR measurements and electrochemical analyses. Influence of the two substituents is discussed. 相似文献
9.
Three palladium(II) complexes have been synthesized, using 3,4-bis(cyanamido) cyclobutane-1,2-dione dianion (3,4-bis(cyanamido)squarate or 3,4-NCNsq2−): [Pd(en)(3,4-NCNsq)] · 1.5H2O (1) (en=1,2-diaminoethane), [Pd(en)(3,4-(NC(O)NH2)sq)] · 0.5H2O (2) and K3Na[Pd2(3,4-(NCN)2sq)4] · 5H2O (3). Complex 1 has been characterized by elemental analysis, IR and 13C NMR spectroscopies. Complexes 2 and 3 have been characterized by single-crystal X-ray diffraction. In complex 2, the unusual hydration of the cyanamido ligand was observed, it proceeds in the coordination sphere of the palladium and leads to a chelating urea squarate ligand. Complex 3 is an anionic dinuclear complex containing four bridging cyanamido squarate ligands. In complexes 2 and 3, the 3,4-NCNsq2− ligand (hydrated or not) is, for the first time, coordinated to the metal atom by the two amido nitrogen atoms, either in a chelating mode (complex 2) or in a bridging mode giving a short Pd ? Pd distance of 2.8866(15) Å (complex 3). Electrochemical studies in acetonitrile and dmf solutions have been performed on complexes 1 and 3. 相似文献
10.