全文获取类型
收费全文 | 256篇 |
免费 | 18篇 |
国内免费 | 43篇 |
出版年
2024年 | 2篇 |
2023年 | 2篇 |
2022年 | 5篇 |
2021年 | 8篇 |
2020年 | 3篇 |
2019年 | 6篇 |
2018年 | 3篇 |
2017年 | 6篇 |
2016年 | 8篇 |
2015年 | 9篇 |
2014年 | 14篇 |
2013年 | 10篇 |
2012年 | 23篇 |
2011年 | 19篇 |
2010年 | 17篇 |
2009年 | 19篇 |
2008年 | 21篇 |
2007年 | 14篇 |
2006年 | 12篇 |
2005年 | 9篇 |
2004年 | 10篇 |
2003年 | 10篇 |
2002年 | 10篇 |
2001年 | 9篇 |
2000年 | 7篇 |
1999年 | 6篇 |
1998年 | 7篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1993年 | 2篇 |
1990年 | 3篇 |
1986年 | 2篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1974年 | 4篇 |
1971年 | 1篇 |
1967年 | 1篇 |
1966年 | 1篇 |
1964年 | 1篇 |
1957年 | 1篇 |
1951年 | 1篇 |
1940年 | 2篇 |
1937年 | 1篇 |
1932年 | 1篇 |
1930年 | 1篇 |
1929年 | 2篇 |
1928年 | 1篇 |
1926年 | 1篇 |
1924年 | 2篇 |
排序方式: 共有317条查询结果,搜索用时 15 毫秒
1.
34Fusarium graminearum Schw isolates produced 4-deoxynivalenol to form significant amounts of 4, 7 — dideoxynivalenol and lesser amounts of 4 — deoxynivalenol monoacetates on grain substratesin vitro. This is the first report on the capability a large group of naturally occurring isolates to produce 4,7-dideoxynivalenol. The average levels of 4,7-dideoxynivalenol on rice, corn, barley, and wheat as a substrate were respectively 26.8, 14.0, 12.8, and 10.5% of the level of 4-deoxynivalenol. 4, 7 — dideoxynivalenol was present in all examined naturally contaminated wheat kernel samples at levels of 1.7 to 7.9% of the level of 4-deoxynivalenol. These findings suggest that more attention should be given to the occurrence of 4,7-dideoxynivalenol in cereals. 相似文献
2.
Variation in the ribosomal internal transcribed spacers (ITS1 and ITS2) among eight taxa of the Mimulus guttatus species complex 总被引:1,自引:0,他引:1
The internal transcribed spacer region (ITS1 and ITS2) of the 18S-25S
nuclear ribosomal DNA sequence and the intervening 5.8S region were
sequenced from three individuals in each of eight taxa of the Mimulus
guttatus species complex. Three discrete variants, or "types," of ITS
sequences were found, among which 30%-40% of sites differed, compared with
1%-2% within types. Dot plots indicate that these types were not related by
conspicuous rearrangements or inversions. More than one ITS type was often
found in the same taxon, and two of three ITS types span species
boundaries, indicating their presence prior to speciation. These ITS
sequences showed essentially no positional homology with the nearest
sequenced relative, tomato. In contrast, the 5.8S region was relatively
unvaried, with 8 of 162 sites varied in the sample among all eight taxa.
The phylogeny inferred by the most common ITS sequence type, rooted by the
two other ITS types, agreed with isozymes in showing the distinctness of M.
nudatus, M. laciniatus, and M. tilingii from the other five taxa.
相似文献
3.
4.
5.
6.
7.
报道了小菇科小菇属真菌10个中国新记录种,香菌组:橙盖小菇Mycena aurantiidisca、黄白小菇Mycena flavoalba、粉黄小菇Mycena floridula;棘刺组:异刺小菇Mycena heteracantha;纤柄组:碱味小菇Mycena amygdalina;脆足组:粉被小菇Mycena zephirus;冬生组:绣线菊小菇Mycena speirea、冬生小菇Mycena hiemalis;小菇组:绒柄小菇Mycena flos-nivium,分别来自吉林等11个省份、自治区。提供了每个物种的形态描述和线条图,以及与相近种的讨论。共计90条自测及下载ITS序列,在采用贝叶斯法和最大似然法构建的小菇属系统发育树中,新记录种均得到分子数据支持。凭证标本存放于吉林农业大学菌物标本馆(HMJAU)。 相似文献
8.
David A. Rolls Rachel Sacks-Davis Rebecca Jenkinson Emma McBryde Philippa Pattison Garry Robins Margaret Hellard 《PloS one》2013,8(11)
Hepatitis C virus (HCV) chronically infects over 180 million people worldwide, with over 350,000 estimated deaths attributed yearly to HCV-related liver diseases. It disproportionally affects people who inject drugs (PWID). Currently there is no preventative vaccine and interventions feature long treatment durations with severe side-effects. Upcoming treatments will improve this situation, making possible large-scale treatment interventions. How these strategies should target HCV-infected PWID remains an important unanswered question. Previous models of HCV have lacked empirically grounded contact models of PWID. Here we report results on HCV transmission and treatment using simulated contact networks generated from an empirically grounded network model using recently developed statistical approaches in social network analysis. Our HCV transmission model is a detailed, stochastic, individual-based model including spontaneously clearing nodes. On transmission we investigate the role of number of contacts and injecting frequency on time to primary infection and the role of spontaneously clearing nodes on incidence rates. On treatment we investigate the effect of nine network-based treatment strategies on chronic prevalence and incidence rates of primary infection and re-infection. Both numbers of contacts and injecting frequency play key roles in reducing time to primary infection. The change from “less-” to “more-frequent” injector is roughly similar to having one additional network contact. Nodes that spontaneously clear their HCV infection have a local effect on infection risk and the total number of such nodes (but not their locations) has a network wide effect on the incidence of both primary and re-infection with HCV. Re-infection plays a large role in the effectiveness of treatment interventions. Strategies that choose PWID and treat all their contacts (analogous to ring vaccination) are most effective in reducing the incidence rates of re-infection and combined infection. A strategy targeting infected PWID with the most contacts (analogous to targeted vaccination) is the least effective. 相似文献
9.
10.
本文通过Aβ25-35诱导体外原代培养的SD乳大鼠海马神经元,建立Aβ毒性损伤细胞模型,结合AnnexinV-FITC/PI荧光双染法流式细胞术、MTT比色法、实时荧光定量PCR及Western blot方法检测川芎嗪(tetrameth-ylpyrazine,TMP)对原代培养的海马神经元细胞活性、早期凋亡率和Bax、Bcl-2基因表达的影响。结果显示川芎嗪高、中剂量可明显增强细胞活性,增加神经元细胞的存活率(P<0.01),可显著抑制海马神经元细胞早期凋亡(P<0.01),抑制凋亡蛋白Bax的表达(P<0.01),增强抗凋亡蛋白bcl-2的表达(P<0.01)。川芎嗪可通过调节Bax/Bcl-2平衡抵抗Aβ25-35诱导的海马神经元凋亡,降低Aβ的神经元毒性,对海马神经元损伤有明显的保护作用。 相似文献