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1.
Lucia Di Giambattista P. Grimaldi S. Gaudenzi D. Pozzi M. Grandi S. Morrone I. Silvestri A. Congiu Castellano 《European biophysics journal : EBJ》2010,39(6):929-934
We have made a preliminary analysis of the results about the effects on tumoral cell line (lymphoid T cell line Jurkat) induced
by UVB radiation (dose of 310 mJ/cm2) with and without a vegetable mixture. In the present study, we have used two techniques: Fourier transform infrared spectroscopy
(FTIR) and flow cytometry. FTIR spectroscopy has the potential to provide the identification of the vibrational modes of some
of the major compounds (lipid, proteins and nucleic acids) without being invasive in the biomaterials. The second technique
has allowed us to perform measurements of cytotoxicity and to assess the percentage of apoptosis. We already studied the induction
of apoptotic process in the same cell line by UVB radiation; in particular, we looked for correspondences and correlations
between FTIR spetroscopy and flow cytometry data finding three highly probable spectroscopic markers of apoptosis (Pozzi et
al. in Radiat Res 168:698–705, 2007). In the present work, the results have shown significant changes in the absorbance and
spectral pattern in the wavenumber protein and nucleic acids regions after the treatments. 相似文献
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Inhibition of growth of Leishmania mexicana mexicana by Leishmania mexicana amazonensis during "in vitro" co-cultivation 总被引:1,自引:0,他引:1
Inhibition of one Leishmania subspecies by exometabolites of another subspecies, a phenomenon not previously reported, is suggested by our recent observations in cell cloning experiments with Leishmania mexicana mexicana and Leishmania mexicana amazonensis. Clones were identified using the technique of schizodeme analysis. The phenomenon observed is clearly relevant to studies of parasite isolation, leishmanial metabolism, cross-immunity and chemotherapy. 相似文献
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Carmen Di Franco Claudio Pisano Patrizio Dimitri Silvia Gigliotti Nikolaj Junakovic 《Chromosoma》1989,98(6):402-410
The genomic distribution of elements of the copia, 412, B 104, mdg 1, mdg 4 and 1731 transposon families was compared by the Southern technique in DNA preparations extracted from brains, salivary glands and adult flies of two related Drosophila lines. The copia, 412 and mdg 1 sequences were also probed in DNA from sperm, embryos, and 1st and 2nd instar larvae. The homogeneity of the patterns observed shows that somatic transposition is unlikely to occur frequently. A correlation between mobility and the euchromatic or heterochromatic location of transposable elements is discussed. In addition, an explanation of the variable band intensities of transposable elements in Southern autoradiographs is proposed. 相似文献
7.
Interleukin 1 beta inhibition of TRH-stimulated prolactin secretion and phosphoinositides metabolism
G Schettini E Landolfi M Grimaldi O Meucci A Postiglione T Florio C Ventra 《Biochemical and biophysical research communications》1989,165(1):496-505
The effect of interleukin 1 beta on prolactin secretion and on phosphoinositide turnover in anterior pituitary cells was evaluated. Interleukin 1 beta significantly inhibited TRH-stimulated prolactin secretion assessed by the reverse hemolytic plaque assay. In particular, the cytokine reduced the percentage of plaque forming cells, the plaque mean area, the large plaques percentage. TRH-stimulated inositol phosphate production was also significantly inhibited by interleukin 1 beta. This study shows that interleukin 1 beta reduces TRH-induced prolactin secretion through a direct action on pituitary cell, and attenuates the TRH-stimulated phosphoinositide breakdown. This latter effect may suggest that the reduced lactotropes sensitivity to TRH action may be partially due to interleukin 1 beta inhibition of phosphatidylinositol breakdown. 相似文献
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In non-specific and rheumatoid synovitis, the use of specific monoclonal antibodies against antigenic determinants of cells of the immune system showed that the characteristic changes of rheumatoid synovitis are located in the synovial internal layers. The monocytes were OKM1, OKM5, S100, OKDR positive, while the subintimal monocytes in non-specific synovitis were OKDR negative. We suggest that, in rheumatoid synovitis, the previously activated monocytes are transported by the bloodstream and pass through the so-called "sinovial barrier" to arrive in the subintimal layers ready to interact with T helper lymphocytes and initiate the immune response mechanisms responsible for lesions in rheumatoid synovitis. 相似文献
10.
Effect of acute and chronic hypernatremia on myoinositol and sorbitol concentration in rat brain and kidney 总被引:5,自引:0,他引:5
In animal models of hypernatremia, increases in brain electrolyte content account for the entire increase in osmolality in acute but not chronic hypernatremia, suggesting that there is generation of additional intracellular solutes ("idiogenic osmoles") in chronic hypernatremic states. In the present study, the concentration of the polyols myoinositol and sorbitol and water content were determined in the brain and kidneys of rats made acutely (2 hours) and chronically (72 hours) hypernatremic by intraperitoneal injection of NaCl and water restriction. Both the brain and the kidney responded to chronic hypernatremia with increased levels of myoinositol. Sorbitol levels increased in the kidney in response to both acute and chronic hypernatremia. Water content dropped in acute hypernatremia, but remained unchanged during chronic hyperosmolar challenge. We conclude that the polyols, myoinositol and sorbitol, may play a significant role in cellular osmoregulation in brain and kidney during chronic hypernatremia in the rat. 相似文献