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Vikas Yadav Patade Sujata Bhargava Penna Suprasanna 《Plant Cell, Tissue and Organ Culture》2012,108(2):279-286
In order to discriminate between the ionic and osmotic components of salt stress, sugarcane (Saccharum officinarum L. cv. Co 86032) calli were cultured on media containing NaCl or polyethylene glycol (PEG) 8000 that exerted the same osmotic
pressure (−0.7 MPa). PEG stress exposure for 15 days led to significant growth reduction and loss in water content than salt
stressed and control tissues. Osmotic adjustment (OA) was observed in callus tissues grown on salt, but was not evident in
callus grown on PEG. Oxidative damage to membranes, estimated in terms of accumulation of thiobarbituric acid reactive substances-TBARS
and electrolytic leakage was significantly higher in both the stressed calli than the control however, the extent of damage
was more in the PEG stressed calli. The stressed callus tissues showed inhibition of ascorbate peroxidase activity, while
catalase activity was increased. These results indicate sensitivity of cells to PEG-mediated stress than salt stress and differences
in their OA to these two stress conditions. The sensitivity to the osmotic stress indicate that expression of the stress tolerance
response requires the coordinated action of different tissues in a plant and hence was not expressed at the cellular level. 相似文献
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Ashish Kumar Srivastava Vinayak H. Lokhande Vikas Y. Patade Penna Suprasanna Rinaldi Sjahril Stanislaus Francis D’Souza 《Acta Physiologiae Plantarum》2010,32(6):1135-1144
The present study was aimed to evaluate the effect of different seed priming methods to enhance the sodium chloride (NaCl)
and polyethylene glycol-8000 (PEG-8000) stress tolerance in Indian mustard (Brassica juncea L.). Seeds subjected to different priming treatments such as water (hydro-priming), calcium chloride (CaCl2) (chemo-priming), and abscisic acid (ABA) (hormonal-priming) showed increased rate of germination as compared to non-primed
seeds. The primed and non-primed seeds were grown for 15 days and then the seedlings were independently subjected to iso-osmotic
salt (150 mM NaCl) or PEG-8000 (20%) stress. The different biochemical responses were studied 10 days after treatment. Under
NaCl and PEG stress, the dry weight and total chlorophyll content were higher in primed sets as compared to non-primed treatment
which was also evident by the phenotype of the seedlings. In general, the higher activities of superoxide dismutase and glutathione
reductase resulted in lower oxidative damage, in terms of malondialdehyde content, under NaCl and PEG stress in hydro-primed
set as compared to non-primed, ABA-, and CaCl2-primed treatments. Besides, the level of total phenolics and accumulation of osmolytes such as free proline, glycine betaine,
and total soluble sugars was also lower in hydro-primed set as compared to other primed and non-primed treatments. The study
thus suggests the use of hydro-priming as a simple and cost-effective strategy to alleviate the NaCl and PEG induced stress
in B. juncea. 相似文献
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In vitro responses of embryogenic sugarcane (Saccharum officinarum L.; cv. CoC-671) calli stressed with different levels of NaCl (0.0, 42.8, 85.6, 128.3, 171.1, 213.9 or 256.7 mM) were studied.
The results showed that a significant decrease in callus growth and cell viability occurred with ≥85.6 mM NaCl. Higher amounts
of free proline and glycine betaine were accumulated in NaCl-stressed calli. Although the leached and retained Na+ contents increased, the retained K+ content decreased with increasing levels of NaCl. Such a mechanism implies that sugarcane can be considered as a Na+-excluder. The accumulation of salt ions and osmolytes could play an important role in osmotic adjustment in sugarcane cells
under salt stress. 相似文献
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Atul Grover Sadhana Singh Pankaj Pandey Vikas Yadav Patade Sanjay Mohan Gupta M. Nasim 《Molecular biology reports》2014,41(11):7479-7489
We report elevated biomass and altered growth characteristics of tobacco plants up on transformation with a NAC (NAM, ATAF1/2,CUC2) gene (GenBank Accession FJ754254) isolated from Lepidium latifolium L. (LlaNAC). Transgenic plants showed significant differences in fresh weight, midrib length of longest leaf, leaf area, height of the plant, root and shoot weights, etc. during vegetative phase. On 100th day after sowing (DAS), plants of transgenic lines were 2–3 times taller than the wild type plants, though no significant difference was recorded in moisture contents of any of the plant tissues. Over-expression of NAC gene up to 2,000 fold was recorded in leaves of transgenic plants on 100th DAS. Interestingly, transgenic plants showed significantly shortened (P(t) = 0.02–0.04) life cycle, as they showed a completely altered growth behaviour. Transgenic plants entered reproductive phase earlier by 60 days, with lines NC2 and NC7b entering first, followed by line NC10. However, the time period spent in the reproductive phase by the plant was nearly twice in case of transgenic lines NC2, NC7b and NC10, as compared to the wild type plants. Despite that, these lines completed their life cycle in 45–60 days lesser than the time taken by wild-type tobacco plants. No difference was recorded in fruit and seed yield of transgenic or wild type plants. To the best of our knowledge, this is the first report on over-expression of NAC gene causing altered growth and biomass patterns. We expect this study to become an important reference towards future engineering of plants for fuel and fodder purposes. 相似文献
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Vinayak H. Lokhande Tukaram D. Nikam Vikas Y. Patade Mahendra L. Ahire P. Suprasanna 《Plant Cell, Tissue and Organ Culture》2011,104(1):41-49
The effect of optimal and supra-optimal concentrations (0, 200, 400 or 600 mM) of NaCl on the growth, osmotic adjustment and
antioxidant enzyme defence was studied in the in vitro cultures of Sesuvium portulacastrum. A significant increase in growth, tissue water content (TWC) and fresh to dry weight ratio (FW/DW) was observed in the shoots
exposed to 200 mM salt. Minimum damage to the membrane in terms of low relative electrolytic leakage (REL) and malondialdehyde
(MDA) content and better osmotic adjustment at 200 mM salt stress was coupled with the higher accumulation of sodium ions
and total soluble sugars as against low proline and glycine betaine contents. A fine tuning of antioxidant enzyme activities
(superoxide dismutase, catalase and ascorbate peroxidase) was also found to be responsible for the optimum growth of shoots.
In contrast, sub-optimal (0 mM) and supra-optimal concentrations (400–600 mM) of NaCl significantly affected the growth, water
status and increased the REL as well as MDA content of the shoots due to the accumulation of toxic concentrations of saline
ions. The highest accumulation of proline and glycine betaine in addition to antioxidant enzyme activities exhibited higher
osmotic adjustment and survival of the shoots under sub- or supra-optimal concentrations of NaCl as a penalty to reduced growth. 相似文献
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