Mechanism of ethonium effect on membrane preparations of brain Na+, K+-ATPase

The effect of ethonium, a local anesthetic, on the membrane preparations of brain N+, K+-ATPase was studied by fluorescent, radioisotopic, electron-microscopic and electrophysiological methods. Ethonium is established to affect the formation of an intermediate phosphorylated product and has no pronounced destructive effect on the membrane. It changes the fluorescence intensity of 2-toluidinonaphthalen-6-sulphonate (TNS), astrafloxin (AF) and fluorene probes in a suspension of the Na+, K+-ATPase preparations, which evidences for ethonium-induced changes in the structure of membrane fragments.     

Myocytes of the inguinal lymph nodes

Owing to the method for making total plane preparations of the capsule after A. V. Borisov, it is possible not only to prove presence of myocytes in the capsule and in the trabeculae of the inguinal lymph nodes in the man and rat, but to open out general regularities of their distribution and orientation. In the capsule areas, corresponding to the places, where the lymph nodules are adjacent to (zone A), the number of myocytes is the least. They are oriented in various directions and are in close contact with each other (fascicular-reticulate principle of distribution of myocytes). In the capsule areas, surrounding A zones (named B zones) the myocytes are situated in tight layers and have circulatory orientation. At the place where the afferent lymphatic vessel gets into the capsule, precapsular lymphangion makes an infundibular dilatation and its myocytes along the sloping spiral get into the capsule, where they are arranged circulatory and form a muscular "constrictor". While studying ultrastructure of myocytes in the rat inguinal lymph nodes, it has been found out that their structure is typical for the smooth muscle cells. There are numerous myo-myocytic contacts of nexus type, that unite the myocytes of the node into a single functional complex.     

Y/15 and Y/22 Derivative Chromosomes in Couples with Reproductive Failures: Algorithm of Preimplantation Genetic Testing and Specificity of Inheritance

Russian Journal of Genetics - The present study reports on the results of preimplantation genetic testing and IVF outcomes in four families, where one of the spouses is a carrier of Y/15 or Y/22...     

Transformations of the Chromophore in the Course of Maturation of a Chromoprotein from Actinia equina

Russian Journal of Bioorganic Chemistry - Currently, it is generally accepted that chromophore synthesis of red fluorescent proteins proceeds via an intermediate “blue” form with a...