全文获取类型
收费全文 | 1935篇 |
免费 | 156篇 |
出版年
2022年 | 9篇 |
2021年 | 29篇 |
2020年 | 15篇 |
2019年 | 17篇 |
2018年 | 24篇 |
2017年 | 22篇 |
2016年 | 45篇 |
2015年 | 74篇 |
2014年 | 94篇 |
2013年 | 100篇 |
2012年 | 162篇 |
2011年 | 152篇 |
2010年 | 93篇 |
2009年 | 97篇 |
2008年 | 118篇 |
2007年 | 132篇 |
2006年 | 112篇 |
2005年 | 118篇 |
2004年 | 94篇 |
2003年 | 101篇 |
2002年 | 96篇 |
2001年 | 29篇 |
2000年 | 10篇 |
1999年 | 24篇 |
1998年 | 26篇 |
1997年 | 24篇 |
1996年 | 25篇 |
1995年 | 27篇 |
1994年 | 21篇 |
1993年 | 20篇 |
1992年 | 28篇 |
1991年 | 18篇 |
1990年 | 20篇 |
1989年 | 6篇 |
1988年 | 8篇 |
1987年 | 11篇 |
1986年 | 8篇 |
1985年 | 7篇 |
1984年 | 5篇 |
1983年 | 6篇 |
1979年 | 11篇 |
1978年 | 7篇 |
1977年 | 3篇 |
1975年 | 3篇 |
1973年 | 3篇 |
1972年 | 3篇 |
1968年 | 5篇 |
1967年 | 3篇 |
1966年 | 3篇 |
1964年 | 4篇 |
排序方式: 共有2091条查询结果,搜索用时 15 毫秒
1.
2.
A Schellenberger I Heinroth G Hübner 《Hoppe-Seyler's Zeitschrift für physiologische Chemie》1967,348(5):506-511
3.
Summary We have made pairwise comparisons between the coding sequences of 21 genes from coldblooded vertebrates and 41 homologous sequences from warm-blooded vertebrates. In the case of 12 genes, GC levels were higher, especially in third codon positions, in warm-blooded vertebrates compared to cold-blooded vertebrates. Six genes showed no remarkable difference in GC level and three showed a lower level. In the first case, higher GC levels appear to be due to a directional fixation of mutations, presumably under the influence of body temperature (see Bernardi and Bernardi 1986b). These GC-richer genes of warm-blooded vertebrates were located, in all cases studied, in isochores higher in GC than those comprising the homologous genes of cold-blooded vertebrates. In the third case, increases appear to be due to a limited formation of GC-rich isochores which took place in some cold-blooded vertebrates after the divergence of warm-blooded vertebrates. The directional changes in the GC content of coding sequences and the evolutionary conservation of both increased and unchanged GC levels are in keeping with the existence of compositional constraints on the genome. 相似文献
4.
Pyruvate decarboxylase (PDC) contains thiamine pyrophosphate (TPP) and Mg2+ as cofactors. 31P NMR studies with PDC in the presence of added Mn2+ reveal the pyrophosphate moiety of TPP to be a nonaccessible area for the external Mn2+ and thus proving the Mg-P-complex (taking part in the binding of the coenzyme to the protein) to be a nonaccessible area for the medium. Glyoxylic acid, acting as an inhibitor of PDC by forming a noncleavable bond with the catalytic center of TPP causes a steric immobilization of the coenzyme indicated by a line broadening of the pyrophosphate moiety. 相似文献
5.
Using two independent methods, it was shown that pyruvate decarboxylase of brewer's yeast/EC 4.1.1.1./showing a sigmoidal v/S-shape and a corresponding activation phase in the product formation is potentially inactive in the absence of its substrate-pyruvate. 相似文献
6.
Similarity of activation of yeast phosphofructokinase by AMP and fructose-2,6-bisphosphate 总被引:1,自引:0,他引:1
K Nissler A Otto W Schellenberger E Hofmann 《Biochemical and biophysical research communications》1983,111(1):294-300
Phosphofructokinase from yeast is effectively activated by AMP and fructose-2,6-bisphosphate by increasing the affinity of the enzyme to fructose-6-phosphate and the maximum activity toward this substrate. The enzyme is activated by AMP and fructose-2, 6-bisphosphate both at high and at low concentrations of ATP. The half maximum stimulation concentrations of AMP and fructose-2, 6-bisphosphate are about 200 microM and 2 microM, respectively. At saturating concentrations of AMP and fructose-2, 6-bisphosphate similar maximum activities were observed in the dependence of enzyme activity on the concentrations of fructose-6-phosphate. The fructose-6-phosphate affinity is more enhanced by fructose-2, 6-bisphosphate than by AMP. 相似文献
7.
8.
9.
10.