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排序方式: 共有141条查询结果,搜索用时 15 毫秒
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Manoj Kumar Abhishek Kumar Dwivedy Parismita Sarma M. S. Dkhar H. Kayang Richa Raghuwanshi 《Plant biosystems》2020,154(3):269-276
AbstractThe study recommends the Artemisia nilagirica (Clarke) Pamp. essential oil (ANEO) as plant-based shelf-life enhancer of millets against fungal, aflatoxin B1 (AFB1) contamination and lipid peroxidation with favourable safety profile. Chemical characterisation of ANEO through GC-MS, recorded 1,5-heptadiene-4-one,3,3,6-trimethyl (32.72%)as the main compound, followed by Artemisia alcohol (13.40%), alpha lonone (4.55%), benzene, methyl (1-methylethyl) (2.97%) and 1-cyclohexene-1-carboxaldehyde,4-(1-methylethyenyl) (2.23%). The mycoflora analysis of millet samples showed Aspergillus flavus strain[LHP(R)-5] as the most AFB1 secreting strain. The ANEO inhibited growth and AFB1 production by the toxigenic strain at 1.4 and 1.0?µL?mL?1, respectively, and also possess broad fungitoxic spectrum. The decrement in membrane ergosterol content, enhanced leakage of cellular Ca2+, K+ and Mg2+ ions along with SEM and TEM study of ANEO-treated cell denotes plasma membrane as antifungal site of action. The ANEO also showed strong antioxidant activity as determined by DPPH? and ABTS?+ assays having IC50 value 2.51 and 1.07?µL?mL?1, respectively. More than 70.78% protection of Ragi samples from fungal contamination was observed during in situ trial. The ANEO showed favourable safety profile with high LD50 value (7528.10?µL?kg?1) for male mice and also exhibited non-phytotoxicity for Ragi seeds germination. 相似文献
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Jayanta Kumar Nath Minakshi Kalita Anu Saikia Hrishikesh Choudhury D. Khlur B. Mukhim Dandadhar Sarma 《Zeitschrift fur angewandte Ichthyologie》2021,37(2):268-277
The present study provides the first detailed early embryonic development of the Shalyni barb, Pethia shalynius (Yazdani & Talukdar, 1975), a vulnerable cyprinid fish occurring in streams and lentic waters of Meghalaya, northeast India. Induced spawning by synthetic hormone injection in May 2019 was conducted to a pair of mature female and male P. shalynius under controlled conditions in a well-aerated aquarium. Fertilized eggs were spherical, 0.75–0.80 mm (approx.) in diameter, transparent, unpigmented and non-adhesive. A total of 22 developmental stages could be categorized under seven broad periods, viz. the zygote, cleavage, blastula, gastrula, segmentation, pharyngula and hatchling. The first cleavage occurred at 15 min post fertilization (mpf), followed by blastulation at 01:23 hr post-fertilization (hpf), gastrulation at 04:20 hpf, initial somite formation at 07:00 hpf, and pharyngula period at 19:20 hpf, respectively. Embryos hatched between 26–27 hpf and the newly-hatched larvae ranged 2.2–2.5 mm in total length. For naturally-declining populations of this vulnerable fish species, inferences drawn from the present study will help provide a baseline data for its conservation and management, and aid the research fields of developmental biology, biotechnology, molecular biology as well as taxonomy of this species. 相似文献
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Saikia Snigdha Pal Uttariya Kalita Deep Jyoti Rai Avdhesh Kumar Sarma Anupam Kataki Amal Chandra Limaye Anil Mukund 《Molecular biology reports》2021,48(7):5399-5409
Molecular Biology Reports - RUNX1T1 is extensively studied in the context of AML1-RUNX1T1 fusion protein in acute myeloid leukemia. Little is known about the function of RUNX1T1 itself, although... 相似文献
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ABSTRACT Background : Cobra bite is frequently reported across the Indian subcontinent and is associated with a high rate of death and morbidity. In eastern India (EI) Naja naja and Naja kaouthia are reported to be the two most abundant species of cobra. Research design and methods : The venom proteome composition of N. naja (NnV) and N. kaouthia (NkV) from Burdwan districts of EI were compared by separation of venom proteins by 1D-SDS-PAGE followed by LC-MS/MS analysis of protein bands. The potency of commercial polyantivenom (PAV) was assessed by neutralization, ELISA, immuno-blot and venom-PAV immunoaffinity chromatography studies. Results : Proteomic analysis identified 52 and 55 proteins for NnV and NkV, respectively, when searched against the Elapidae database. A small quantitative difference in venom composition between these two species of cobra was observed. PAVs exhibited poor cross-reactivity against low molecular mass toxins (<20 kDa) of both cobra venoms, which was substantiated by a meager neutralization of their phospholipase A2 activity. Phospholipase A2 and 3FTx, the two major classes of nonenzymatic and enzymatic proteins, respectively, were partially recognized by PAVs. Conclusions : Efforts must be made to improve immunization protocols and supplement existing antivenoms with antibodies raised against the major toxins of these venoms. 相似文献
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Kumar Alok Kalita J. Sinha Rohit A. Singh Gajendra B Anjum Shukla Mukti Tiwari Swasti Dhole T. N. Misra U. K. 《Neurochemical research》2020,45(9):2184-2195
Neurochemical Research - Role of autophagy in Japanese encephalitis viral (JEV) infection is not well known. In the present study, we reported the role of autophagy flux in microglia activation,... 相似文献
7.
The vegetative growth of Ulva lactuca was studied to determine if the growth rate of the alga is driven by infradian rhythmicity. The influence of temperature on the infradian rhythm of growth was also investigated. Discs of Ulva were grown in controlled laboratory conditions at different combinations of temperature (5, 10, 15, 20°С) and irradiance (40 and 60 μmol photons m?2 s?1) under 12 : 12 h light : dark cycles. The growth rates exhibited a rhythmic pattern with one major peak every 2 or 3 days. Growth at 5 or 10°С increased the prevalence of 3-day cycles and maintained U. lactuca in the vegetative growth stage. In contrast, growth at 15 or 20°С provoked a predominance of the 2-day cycle and induced reproduction. The 2- or 3-day cycles were combined in longer cycles having a period close to 6 days. We suppose that the 2-, 3- and 6-day rhythms of physiological processes are related to large-scale Rossby and Kelvin waves, which produce oscillations in the geomagnetic field and seawater temperature with the same periods. The predominance of 2-day or 3-day fluctuations of the geomagnetic field and temperature probably determine the prevalence of reproduction and vegetative growth, respectively, in Ulva. 相似文献
8.
Boruwa J Kalita B Barua NC Borah JC Mazumder S Thakur D Gogoi DK Bora TC 《Bioorganic & medicinal chemistry letters》2004,14(13):3571-3574
The absolute stereochemistry of the new antifungal and antibacterial antibiotic produced by Streptomyces sp.201 has been established by achieving the total synthesis of the product. A series of analogues have also been synthesized by changing the side chain and their bioactivity assessed against different microbial strains. Among them, 1e (R = C8H17) was found to be the most potent with MIC of 8 microg/mL against Mycobacterium tuberculosis, 12 microg/mL against Escherichia coli and 16 microg/mL against Bacillus subtilis 6 microg/mL against Proteus vulgaris. This was followed by 1b (R = C5H11) with MIC of 10-20 microg/mL range and 1d (R = C7H15) with MIC of 14-24 g/mL, whereas 1a (R = C4H9) and 1f (R = C18H35) were found to be completely inactive. Besides, 1c (R = C6H13) showed certain extent of antibacterial activity in the range of 24-50 microg/mL. Mycobacterium tuberculosis was very sensitive to 1e (R = C8H17) with MIC of 8 microg/mL. Antifungal activity of analogues 1d (R = C7H15) and 1e, (R = C8H17) against Fusarium oxysporum and Rhizoctonia solani were found promising with MFCs in the 15-18 microg/mL range. 相似文献
9.
Vaisburg A Bernstein N Frechette S Allan M Abou-Khalil E Leit S Moradei O Bouchain G Wang J Woo SH Fournel M Yan PT Trachy-Bourget MC Kalita A Beaulieu C Li Z MacLeod AR Besterman JM Delorme D 《Bioorganic & medicinal chemistry letters》2004,14(1):283-287
A variety of omega-substituted alkanoic acid (2-amino-phenyl)-amides were designed and synthesized. These compounds were shown to inhibit recombinant human histone deacetylases (HDACs) with IC(50) values in the low micromolar range and induce hyperacetylation of histones in whole cells. They induced expression of p21WAF1/Cip1 and caused cell-cycle arrest in human cancer cells. Compounds in this class showed efficacy in human tumor xenograft models. 相似文献
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