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Identification of a female spawn‐associated Kazal‐type inhibitor from the tropical abalone Haliotis asinina
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Tianfang Wang Parinyaporn Nuurai Carmel McDougall Patrick S. York Utpal Bose Bernard M. Degnan Scott F. Cummins 《Journal of peptide science》2016,22(7):461-470
Abalone (Haliotis) undergoes a period of reproductive maturation, followed by the synchronous release of gametes, called broadcast spawning. Field and laboratory studies have shown that the tropical species Haliotis asinina undergoes a two‐week spawning cycle, thus providing an excellent opportunity to investigate the presence of endogenous spawning‐associated peptides. In female H. asinina, we have isolated a peptide (5145 Da) whose relative abundance in hemolymph increases substantially just prior to spawning and is still detected using reverse‐phase high‐performance liquid chromatography chromatograms up to 1‐day post‐spawn. We have isolated this peptide from female hemolymph as well as samples prepared from the gravid female gonad, and demonstrated through comparative sequence analysis that it contains features characteristic of Kazal‐type proteinase inhibitors (KPIs). Has‐KPI is expressed specifically within the gonad of adult females. A recombinant Has‐KPI was generated using a yeast expression system. The recombinant Has‐KPI does not induce premature spawning of female H. asinina when administered intramuscularly. However it displays homomeric aggregations and interaction with at least one mollusc‐type neuropeptide (LRDFVamide), suggesting a role for it in regulating neuropeptide endocrine communication. This research provides new understanding of a peptide that can regulate reproductive processes in female abalone, which has the potential to lead to the development of greater control over abalone spawning. The findings also highlight the need to further explore abalone reproduction to clearly define a role for novel spawning‐associated peptide in sexual maturation and spawning. Copyright © 2016 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
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Tinikul Y Poljaroen J Nuurai P Anuracpreeda P Chotwiwatthanakun C Phoungpetchara I Kornthong N Poomtong T Hanna PJ Sobhon P 《Cell and tissue research》2011,343(3):579-593
We used antibodies against octopus gonadotropin-releasing hormone (octGnRH) and tunicate GnRH (tGnRH-I) in order to investigate
the existence and distribution of GnRH-like peptides in the central nervous system (CNS) and in the ovary during various stages
of the ovarian cycle of the white shrimp, Litopenaeus vannamei. OctGnRH-immunoreactive and tGnRH-I-immunoreactive neurons and fibers were present in several regions of the supraesophageal
ganglion (brain), subesophageal ganglion (SEG), thoracic ganglia, and abdominal ganglia. In the brain, both octGnRH immunoreactivity
(ir) and tGnRH-I-ir were detected in neurons of clusters 6, 11, 17, and associated fibers, and the anterior medial protocerebral,
posterior medial protocerebral, olfactory, and tegumentary neuropils. In the SEG and thoracic ganglia, octGnRH-immunoreactive
and tGnRH-I-immunoreactive neurons and fibers were present in dorsolateral and ventromedial cell clusters and in surrounding
fibers. Only immunoreactive fibers were detected in the abdominal ganglia. In the ovary, both octGnRH and tGnRH-I were detected
at medium intensity in the cytoplasm of early step oocytes (Oc2) and, at high intensity, in Oc3. Furthermore, octGnRH-ir and
tGnRH-I-ir were intense in follicular cells surrounding Oc2 and Oc3. The presence of GnRH-ir in the CNS and ovary indicates
that GnRH-like peptides occur in the white shrimp, and that GnRHs are involved in the reproductive process, especially ovarian
maturation and the differentiation of oocytes, as reported in other species. 相似文献
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Zhipeng Liu John Donald Peter Hanna Parinyaphon Nuurai Prasert Sobhon 《Invertebrate neuroscience : IN》2009,9(1):21-28
Evidence for the presence of a putative egg-laying (ELH) hormone has been previously described in the black tiger shrimp,
Penaeus monodon, so a further investigation was carried out to detect its presence in a range of Decapoda crustaceans prior to a full molecular
analysis. The crustaceans were represented by the Australian fresh water yabbie, Cherax destructor, the Australian southern rock lobster, Jasus edwardsii, the snow crab, Chionoecetes opilio, and the blue swimmer crab, Portunus pelagicus. Female cerebral ganglia, ventral nerve cords and gonads were investigated in a comparative study of the distribution of
the immunoreactive hormone using immunoenzyme and immunofluorescence techniques. Immunoreactivity was detected in all tissues
of interest, and the distribution patterns showed similarity within the four species, as well as that of P. monodon reported in the earlier study. There were minor variations. These data indicate that a putative ELH-like neuropeptide is
widespread in crustaceans, and supports its previous identification in a range of molluscs and other invertebrates. Elucidation
of the molecular structure of the peptide hormone and its encoding gene, as well as its involvement in spawning behaviour
of crustaceans, is now fully under investigation. 相似文献
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