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排序方式: 共有162条查询结果,搜索用时 125 毫秒
1.
Seyed Sajad Mohseni Salehi Monfared Shirin Pournourmohammadi 《Biological trace element research》2010,133(2):236-241
Islet transplantation has become a promising treatment in the therapy of type 1 diabetes. Its function improvement, after
isolation and before transplantation, is crucial because of their loss both in number and function of islets after isolation
procedures. Trace elements sodium orthovanadate (SOV) and sodium molybdate (SM), as well as medicinal plant Teucrium polium L. (TP), showed and possessed high beneficial antioxidative potential and even hypoglycemic properties via their effect on
islets. We evaluated the effect of these components in combination on cultured islet function in order to improve pancreatic
islet transplantation. Rat pancreatic islets were cultured for 24 h then incubated with different concentrations of TP (0.01
and 0.1 mg/mL) alone and in combination with SOV (1 mM) or SM (1 mM). Insulin concentration in buffer media was measured as
islet secretory function. Administration of TP (0.01 mg/mL), SM, and SOV alone or in combination with each other significantly
increased insulin secretion at high glucose concentration (16.7 mM); insulin secretion was significantly greater in the group
containing both TP and SM than other treated groups (p < 0.05). The combination of the mentioned trace elements especially molybdate with TP could improve islet cells function before
transplantation. 相似文献
2.
Preimplantation embryo development was arrested at the premorula stage in hamsters ovariectomized on Day 1 of pregnancy. This effect was reversed when 500 micrograms progesterone were administered daily. Oestradiol-17 beta given alone had no significant effect on the cleavage rate or blastocyst formation, but a synergistic response was evident when a suboptimal (30 micrograms) dose of progesterone was given with 50 ng oestradiol-17 beta. Cholesterol and hydrocortisol had no effect on embryo development. 相似文献
3.
Embryos recovered from sows on Days 9-13 of pregnancy (Day 0 = first day of estrus) exhibited saturable and time-dependent specific binding of 125I-epidermal growth factor (EGF). The specific binding (pg/mg protein) was greater (P less than 0.001) for Day 13 elongated conceptuses than for conceptuses of earlier stages. Scatchard analyses showed two classes of binding sites (Kd = 7.0 +/- 2.6 x 10(-11) M, Bmax = 6.2 +/- 1.4 fmol/mg protein and Kd = 3.4 +/- 0.2 x 10(-8) M, Bmax = 420 +/- 80 fmol/mg protein). The EGF receptor in Day 13 conceptus membranes is a 170-kDa protein and was phosphorylated in the presence of EGF and adenosine triphosphate. EGF stimulated protein tyrosine kinase activity about 1.6-fold over basal levels. The results show that the preimplantation pig conceptus possesses EGF-binding sites with the properties of functional EGF-receptors. 相似文献
4.
Alemeh Rafaee Anahita Mohseni Meybodi Parichehreh Yaghmaei Seyedeh Hanieh Hosseini Marjan Sabbaghian 《Molecular reproduction and development》2020,87(2):251-259
SEPT12 is a testis‐specific gene involved in the terminal differentiation of male germ cells. SEPT12 protein is required for sperm head‐tail formation and acts as a fundamental constituent of sperm tail annulus. In this study, we screened genetic variations in exons 5, 6, 7 of the SEPT12 and assessed the annulus status in teratozoospermic, globozoospermic, and patients with immotile short tail sperm. DNA sequencing was performed for 90 teratozoospermic and 30 normozoospermic individuals. Immunocytochemistry, transmission electron microscopy and western blotting were conducted to evaluate annulus status and the expression level of SEPT12 in patients with a distinct exonic variation (c.474G>A), respectively. Five polymorphisms identified within the desired regions of the SEPT12, among them c.474G>A had the potential to induce aberrant splicing results in the expression of a truncated protein. The annulus was detected in most of the spermatozoa from teratozoospermic and normozoospermic men with c.474G>A. In contrast, in the patient with short tail sperm defect carrying c.474G>A, 99% of spermatozoa were devoid of the annulus. Based on our findings there would be no association between exons 5, 6, 7 polymorphisms of the SEPT12 gene and the occurrence of mentioned disease but c.474G>A would be a predisposing factor in male infertility. 相似文献
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Nanoparticles of cadmium selenide (CdSe) doped with europium, were synthesized as stabilizing agents using thioglycolic acid ligand. This method is based on the enhancing effect of CdSe quantum dots (QDs) doped with europium on chemiluminescence (CL) emission. This emission was generated by mixing CdSe QDs with manganese (II), iron (II) and chrome (II) sulfates as catalysts in the presence of hydrogen peroxide (H2O2). The structural characteristics and morphology of these nanoparticles were investigated by scanning electron microscopy, Fourier transform infrared spectroscopy, ultraviolet–visible absorption spectroscopy, X‐ray pattern and dynamic light scattering methods. The CdSe QDs doped with europium were used as the sensitizer in a luminol?hydrogen peroxide CL system. The sensitized CdSe QDs were analyzed for antibacterial activity against Gram‐positive or Gram‐negative bacteria. The results showed that the CdSe QDs are effective against all the studied bacteria, effectiveness was especially higher for Bacillus subtilis. 相似文献
8.
Adorian Taida Juliana Jamali Hadi Farsani Hamed Ghafari Darvishi Paria Hasanpour Soleiman Bagheri Tahereh Roozbehfar Reza 《Probiotics and antimicrobial proteins》2019,11(1):248-255
Probiotics and Antimicrobial Proteins - This study was conducted to evaluate different doses of two species of Bacillus (Bacillus licheniformis and Bacillus subtilis), on growth parameters,... 相似文献
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10.
Liu Z Kilburn BA Leach RE Romero R Paria BC Armant DR 《Molecular reproduction and development》2004,68(3):345-353
Blastocyst implantation and placentation require molecular and cellular interactions between the uterine endometrium and blastocyst trophectoderm. Previous studies showed that histamine produced in the mouse uterine luminal epithelium interacts with trophoblast histamine type-2 receptors (H2) to initiate blastocyst implantation. However, it is unknown whether similar histamine activity is operative in humans. Using a human cell line (HTR-8/SVneo) derived from first-trimester cytotrophoblasts that expresses both histamine type-1 receptor (H1) and H2, we found that histamine promotes cytotrophoblast invasiveness specifically through activation of H1. Stimulation of H1 in human cytotrophoblasts by histamine induced intracellular Ca2+ (Ca(2+)i) transients by activating phospholipase C and the inositol trisphosphate pathway. The enhanced invasion induced by histamine was blocked by pretreatment with H1 antagonist or by chelation of Ca(2+)i. These findings suggest possible differences between rodents and humans in histamine signaling to the trophoblast. 相似文献