全文获取类型
收费全文 | 355篇 |
免费 | 41篇 |
国内免费 | 1篇 |
专业分类
397篇 |
出版年
2023年 | 1篇 |
2022年 | 3篇 |
2021年 | 6篇 |
2020年 | 5篇 |
2019年 | 6篇 |
2018年 | 10篇 |
2017年 | 6篇 |
2016年 | 13篇 |
2015年 | 18篇 |
2014年 | 14篇 |
2013年 | 38篇 |
2012年 | 27篇 |
2011年 | 31篇 |
2010年 | 25篇 |
2009年 | 14篇 |
2008年 | 19篇 |
2007年 | 20篇 |
2006年 | 9篇 |
2005年 | 18篇 |
2004年 | 14篇 |
2003年 | 7篇 |
2002年 | 9篇 |
2001年 | 10篇 |
2000年 | 3篇 |
1999年 | 5篇 |
1998年 | 8篇 |
1997年 | 3篇 |
1996年 | 7篇 |
1995年 | 2篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1990年 | 4篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 3篇 |
1985年 | 6篇 |
1983年 | 1篇 |
1982年 | 3篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1975年 | 4篇 |
1974年 | 3篇 |
1968年 | 1篇 |
1966年 | 2篇 |
1965年 | 1篇 |
排序方式: 共有397条查询结果,搜索用时 0 毫秒
1.
2.
In a place conditioning paradigm, rats were exposed to one of two distinctive environments following injection of drug or vehicle. Preference was measured under drug free conditions by allowing subjects free access to both settings and measuring where they spent more time. Comparisons were made between morphine and saline; PCP and saline; and one of several doses of morphine and a standard dose. Morphine was preferred over saline and, when compared to the reference dose, lower doses of morphine were less preferred and higher doses more preferred. PCP was never preferred over saline and under some conditions produced a conditioned place aversion. The ability to generate dose dependent effects with morphine should allow more sophisticated studies in which shifts in dose response curves are required. 相似文献
3.
Beneficial effect of S-adenosyl-L-methionine in lead intoxication. Another approach to clinical therapy 总被引:1,自引:0,他引:1
S R Paredes A A Juknat de Geralnik A M Batlle H A Conti 《The International journal of biochemistry》1985,17(5):625-629
Five patients with chronic lead intoxication were treated with S-adenosyl-L-methionine (12 mg/kg body weight, daily), given intravenously, over a period of 22 days. A significant recovery of erythrocytic ALA-D was observed in all cases, after therapy. Blood lead content significantly pathologic at the beginning of SAM administration, rapidly decreased within 24-48 h of initiating treatment, reaching nearly control values at the end of the trial. A good correlation between recovery of ALA-D activity and decreased concentration of lead in RBC was found. GSH content in blood was diminished in lead poisoned patients, increasing to normal levels after SAM administration. Other biochemical parameters such as Deaminase activity in RBC, ALA, PBG, porphyrins and lead in urine and serum gamma-GT were measured, showing no important deviations from control values before, during or after treatment. Both biochemical and clinical improvement was observed, indicating that SAM therapy is beneficial in the treatment of lead intoxication. No untoward signs were observed. The mechanism of action of SAM is not yet clear; however, a chelating effect could be excluded, and very likely its action can be attributed to glutathione availability. 相似文献
4.
5.
Classical conditioning, decay and extinction of cocaine-induced hyperactivity and stereotypy 总被引:3,自引:0,他引:3
G A Barr N S Sharpless S Cooper S R Schiff W Paredes W H Bridger 《Life sciences》1983,33(14):1341-1351
Following 10 daily pairings of multiple conditioned stimuli with injection of cocaine (15 mg/kg), the presentation of the stimuli alone elicited behaviors in rats similar to those induced by cocaine. The behaviors included increased duration or frequency of rearing, sniffing, head bobbing, and horizontal locomotor activity (crossing). The level of the conditioned response for several of these behaviors approximated that induced by the drug itself. The conditioned drug effect showed decay over 15 days but little extinction during 4 daily trials. Brain concentrations of the dopamine metabolites, homovanillic acid and dihydroxyphenylacetic acid, were similar in the conditioned and pseudoconditioned control groups in both the caudate and mesolimbic areas. The behavioral results demonstrate that, in a classical conditioning paradigm, previously neutral stimuli can elicit behaviors similar to those induced by cocaine and that certain conditioned responses show time related decline. This agrees with the reported conditioning of amphetamine's behavioral effects but differs in terms of the action on brain dopamine turnover. 相似文献
6.
7.
J. Cegarra C. Paredes A. Roig M. P. Bernal D. García 《International biodeterioration & biodegradation》1996,38(3-4)
Vast amounts of olive mill wastewaters (OMW) are produced in Mediterranean countries, where their treatment and disposal are becoming a serious environmental problem. Increasing attention has been paid to discovering a use for OMW and a wide range of technological treatments are available nowadays for reducing their pollutant effects and for their transformation into valuable products, the most suitable procedures being found to involve recycling rather than the detoxication of these wastes. Direct application of OMW to soil has been considered as an inexpensive method of disposal and recovery of their mineral and organic components but, because of their organic acid and phenol contents, OMW are also a source of pollution. By using composting technologies, it is possible to transform either fresh OMW or sludge from pond-stored OMW mixed with appropriate plant waste waterials (carriers) into organic fertilizers (composts) with no phytotoxicity to improve soil fertility and plant production, the process involving the microbial degradation of the polluting load of the wastes. Results of field and pot experiments using OMW-composts to cultivate horticultural and other crops have shown that yields obtained with organic fertilization are similar, and sometimes higher, to those obtained with a balanced mineral fertilizer. A comparison between the macro and micronutrient contents of plants cultivated with organic or mineral fertilizers did not generally reveal important differences. However, the cases of iron and manganese are worth mentioning as their bio-availability may be linked to the soil humic complexes originated by the OMW organic fertilizers. 相似文献
8.
9.
10.
Madelynn Corda Joshua Sciurba Jiana Blaha Siddhartha Mahanty Adriana Paredes Hector H. Garcia Theodore E. Nash Thomas B. Nutman Elise M. OConnell 《PLoS neglected tropical diseases》2022,16(5)
BackgroundAntigen tests for diagnosis and disease monitoring in some types of neurocysticercosis (NCC) are useful but access to testing has been limited by availability of proprietary reagents and/or kits.Methods/Principal findingsThree previously identified IgM-secreting hybridomas whose IgM products demonstrated specificity to Taenia solium underwent variable heavy and light chain sequencing and isotype conversion to mouse IgG. Screening of these recombinantly expressed IgG anti-Ts hybridomas, identified one (TsG10) with the highest affinity to crude Taenia antigen. TsG10 was then used as a capture antibody in a sandwich antigen detection immunoassay in combination with either a high titer polyclonal anti-Ts antibody or with biotinylated TsG10 (termed TsG10*bt). Using serum, plasma, and CSF samples from patients with active NCC and those from NCC-uninfected patients, ROC curve analyses demonstrated that the TsG10-TsG10-*bt assay achieved a 98% sensitivity and 100% specificity in detecting samples known to be antigen positive and outperformed the polyclonal based assay (sensitivity of 93% with 100% specificity). By comparing levels of Ts antigen (Ag) in paired CSF (n = 10) or plasma/serum (n = 19) samples from well-characterized patients with extra-parenchymal NCC early in infection and at the time of definitive cure, all but 2 (1 from CSF and 1 from plasma) became undetectable. There was a high degree of correlation (r = 0.98) between the Ag levels detected by this new assay and levels found by a commercial assay. Pilot studies indicate that this antigen can be detected in the urine of patients with active NCC.Conclusions/SignificanceA newly developed recombinant monoclonal antibody-based Ts Ag detection immunoassay is extremely sensitive in the detection of extra-parenchymal NCC and can be used to monitor the success of treatment in the CSF, serum/plasma and urine. The ability to produce recombinant TsG10 at scale should enable use of this antigen detection immunoassay wherever NCC is endemic.Clinical Trial RegistrationClinicalTrials.gov Identifiers: - & NCT00001205. NCT00001645相似文献