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Susan Morrison Grace John-Stewart John J. Egessa Sezi Mubezi Sylvia Kusemererwa Dennis K. Bii Nulu Bulya Francis Mugume James D. Campbell Jonathan Wangisi Elizabeth A. Bukusi Connie Celum Jared M. Baeten Partners PrEP Study Team 《PloS one》2015,10(10)
During an HIV-1 prevention clinical trial in East Africa, we observed 16 cases of primary HIV-1 infection in women coincident with pregnancy or breastfeeding. Nine of eleven pregnant women initiated rapid combination antiretroviral therapy (ART), despite having CD4 counts exceeding national criteria for ART initiation; breastfeeding women initiated ART or replacement feeding. Rapid ART initiation during primary HIV-1 infection during pregnancy and breastfeeding is feasible in this setting. 相似文献
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Household‐level correlates of children's physical activity levels in and across 12 countries 下载免费PDF全文
Deirdre M. Harrington Fiona Gillison Stephanie T. Broyles Jean‐Philippe Chaput Mikael Fogelholm Gang Hu Rebecca Kuriyan Anura Kurpad Allana G. LeBlanc Carol Maher Jose Maia Victor Matsudo Timothy Olds Vincent Onywera Olga L. Sarmiento Martyn Standage Mark S. Tremblay Catrine Tudor‐Locke Pei Zhao Peter T. Katzmarzyk for the ISCOLE Research Group 《Obesity (Silver Spring, Md.)》2016,24(10):2150-2157
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Twenty-eight strains of pectolytic clostridia were isolated from sugar beet pulp silages. Seventeen non-pigmented strains were presumed to be Clostridium acetobutylicum ; the remaining 11 pigmented strains were similar to Cl. felsineum. The addition of molasses to sugar beet pulps favoured the growth of other bacteria, particularly lactic acid organisms, whereas pectolytic clostridia were only occasionally found. The pectolytic clostridia promoted the structure loss of simulated silages. The use of molasses in sugar beet pulp ensiling was suggested to prevent texture loss of the ensiled mass. 相似文献
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S Papa M Vitale G Mazzotti R Rizzoli M Falconi A Bartoletti F A Manzoli 《Histochemistry》1988,89(3):241-245
A number of techniques are currently employed for the fractionation of heterogeneous cell populations or for the separation of cells in different phases of their cycle. With the development of osmotically inert colloidal silica particles media, density gradient centrifugation became an established method for the separation and purification of cells and subcellular particles. We have applied this technique to the separation of cycling from resting Friend erythroleukemia cells, to obtain purified populations for further biological assays. The flow cytometric analysis of DNA content of the different fractions obtained by the gradient and stained with Propidium Iodide (PI), showed the S compartment highly concentrated in the 1.073/77 g/ml interface, while the upper levels of the gradient were highly enriched of cells in G1 phase. Moreover, the dual parameter analysis of DNA content by means of Bromodeoxyuridine (BrdUrd) incorporation and PI staining, showed that part of the cells in the 1.067/73 fraction represented the early S phase even if their DNA level, measured on the basis of PI fluorescence was within the diploid cell cluster. This method seems to be suitable to obtain pure cell fractions even when dealing with numerically large populations. 相似文献
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Gemma L. Moir-Meyer John F. Pearson Felicity Lose Rodney J. Scott Mark McEvoy John Attia Elizabeth G. Holliday Paul D. Pharoah Alison M. Dunning Deborah J. Thompson Douglas F. Easton Amanda B. Spurdle Logan C. Walker The Australian National Endometrial Cancer Study Group The Hunter Community Study Studies of Epidemiology Risk Factors in Cancer Heredity 《Human genetics》2015,134(3):269-278
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A. Vuillemin D. Ariztegui G. Nobbe C. J. Schubert the Pasado Science Team 《Geomicrobiology journal》2014,31(4):285-298
Methanogenic populations were investigated in subsaline Laguna Potrok Aike sediments, southern Argentina. Microbial density and activity were assessed via cell count and in situ ATP detection for the last ~11K years. Methanogen phylogenetics highlighted species stratification throughout depth, whereas CO2 reduction was the major pathway leading to methane production. Organic substrates, characterized using pore water analysis, bulk organic fractions and saturated fatty acids, showed a clear link between sediment colonization and initial organic sources. Concentrations and δ13C compositions of methane and fatty acids provided final evidence of a microbial imprint on Holocene organic proxies in the most colonized intervals. 相似文献