排序方式: 共有14条查询结果,搜索用时 15 毫秒
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Benedikt Weber Manuel Hora Pamina Kazman Christoph Göbl Carlo Camilloni Bernd Reif Johannes Buchner 《Journal of molecular biology》2018,430(24):4925-4940
The antibody light chain (LC) consists of two domains and is essential for antigen binding in mature immunoglobulins. The two domains are connected by a highly conserved linker that comprises the structurally important Arg108 residue. In antibody light chain (AL) amyloidosis, a severe protein amyloid disease, the LC and its N-terminal variable domain (VL) convert to fibrils deposited in the tissues causing organ failure. Understanding the factors shaping the architecture of the LC is important for basic science, biotechnology and for deciphering the principles that lead to fibril formation. In this study, we examined the structure and properties of LC variants with a mutated or extended linker. We show that under destabilizing conditions, the linker modulates the amyloidogenicity of the LC. The fibril formation propensity of LC linker variants and their susceptibility to proteolysis directly correlate implying an interplay between the two LC domains. Using NMR and residual dipolar coupling-based simulations, we found that the linker residue Arg108 is a key factor regulating the relative orientation of the VL and CL domains, keeping them in a bent and dense, but still flexible conformation. Thus, inter-domain contacts and the relative orientation of VL and CL to each other are of major importance for maintaining the structural integrity of the full-length LC. 相似文献
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Tetraploid triticale, (A/B)(A/B)RR (2n = 28), is a botanical novelty, an amphiploid composed of a diploid rye and a 14 chromosome wheat genome made up of chromosomes of the A and B genomes of tetraploid wheat. Restriction fragment length polymorphism (RFLP) markers were used to elucidate the chromosome composition of the mixed wheat genome of 35 different tetraploid triticale lines. Of 128 possible A/B chromosome pair combinations, only 6 were found among these lines, with a prevalence of the 1A, 2A, 3B, 4B, 5B, 6B, and 7B karyotype. In most triticale lines stable wheat genomes made up of only homologous A or B genome chromosome pairs were identified, however, in some lines homoeologous chromosome pairs were found. In this paper we demonstrate that RFLPs can be used successfully as an alternative to C-banding for the identification of the chromosome composition of tetraploid triticale and discuss the possible selective advantage of specific chromosome composition. 相似文献
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U. Hohmann J. Zoller R. G. Herrmann M. E. Kazman 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(3-4):647-656
Chromosome 1D, which carries the advantageous alleles of glutenin and gliadin, attracts major interest with respect to improving
the bread-making quality of triticale. Eighty-one BC1F4 lines from different primary and secondary hexaploid triticale crosses were selected for 1D chromatin analysis using SDS-PAGE
and C-banding. In situ hybridization and RFLP-based comparative physical mapping of group 1 chromosomes revealed 20 lines
with complete 1D (1A) substitutions. Nine 1D (1B) substitutions, six 1D (1R) substitutions and one 1D addition line were also
selected. Three lines were pure AABBRR hexaploids without any D-genome chromosomes. For the remaining 42 lines (51.8%), a
wide spectrum of 20 different recombinations between chromosomes 1A and 1D was uncovered. Altogether, they were generated
without any earlier irradiation, tissue culture or genetic induction of chromosome pairing. In addition, 14 translocations
between 1B/1D, 1A/1R, 1B/1R, 1D/1R and 1A/1B were detected. Considerable variability for sedimentation values was found, with
the highest sedimentation values among lines with complete 1D chromosomes. The implications of using triticale as a model
for generating compensating chromosome rearrangements in defined homoeologous groups and the breeding potential of D-genome
chromatin introgressed into triticale with improved sedimentation values are discussed.
Received: 27 July 1998 / Accepted: 5 August 1998 相似文献
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M Gowda Y Zhao T Würschum C FH Longin T Miedaner E Ebmeyer R Schachschneider E Kazman J Schacht J-P Martinant M F Mette J C Reif 《Heredity》2014,112(5):552-561
The accuracy of genomic selection depends on the relatedness between the members of the
set in which marker effects are estimated based on evaluation data and the types for which
performance is predicted. Here, we investigate the impact of relatedness on the
performance of marker-assisted selection for fungal disease resistance in hybrid wheat. A
large and diverse mapping population of 1739 elite European winter wheat inbred lines and
hybrids was evaluated for powdery mildew, leaf rust and stripe rust resistance in
multi-location field trials and fingerprinted with 9 k and 90 k SNP arrays.
Comparison of the accuracies of prediction achieved with data sets from the two marker
arrays revealed a crucial role for a sufficiently high marker density in genome-wide
association mapping. Cross-validation studies using test sets with varying degrees of
relationship to the corresponding estimation sets revealed that close relatedness leads to
a substantial increase in the proportion of total genotypic variance explained by the
identified QTL and consequently to an overoptimistic judgment of the precision of
marker-assisted selection. 相似文献
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Population structure, genetic diversity and linkage disequilibrium in elite winter wheat assessed with SNP and SSR markers 总被引:3,自引:0,他引:3
Tobias Würschum Simon M. Langer C. Friedrich H. Longin Viktor Korzun Eduard Akhunov Erhard Ebmeyer Ralf Schachschneider Johannes Schacht Ebrahim Kazman Jochen C. Reif 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(6):1477-1486
Modern genomics approaches rely on the availability of high-throughput and high-density genotyping platforms. A major breakthrough in wheat genotyping was the development of an SNP array. In this study, we used a diverse panel of 172 elite European winter wheat lines to evaluate the utility of the SNP array for genomic analyses in wheat germplasm derived from breeding programs. We investigated population structure and genetic relatedness and found that the results obtained with SNP and SSR markers differ. This suggests that additional research is required to determine the optimum approach for the investigation of population structure and kinship. Our analysis of linkage disequilibrium (LD) showed that LD decays within approximately 5–10 cM. Moreover, we found that LD is variable along chromosomes. Our results suggest that the number of SNPs needs to be increased further to obtain a higher coverage of the chromosomes. Taken together, SNPs can be a valuable tool for genomics approaches and for a knowledge-based improvement of wheat. 相似文献
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Hybrid wheat: quantitative genetic parameters and consequences for the design of breeding programs 总被引:2,自引:0,他引:2
Carl Friedrich Horst Longin Manje Gowda Jonathan Mühleisen Erhard Ebmeyer Ebrahim Kazman Ralf Schachschneider Johannes Schacht Martin Kirchhoff Yusheng Zhao Jochen Christoph Reif 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(11):2791-2801