全文获取类型
收费全文 | 184篇 |
免费 | 18篇 |
国内免费 | 1篇 |
专业分类
203篇 |
出版年
2022年 | 1篇 |
2020年 | 2篇 |
2019年 | 1篇 |
2018年 | 2篇 |
2017年 | 2篇 |
2016年 | 2篇 |
2015年 | 3篇 |
2014年 | 5篇 |
2013年 | 6篇 |
2012年 | 12篇 |
2011年 | 5篇 |
2010年 | 8篇 |
2009年 | 6篇 |
2008年 | 6篇 |
2007年 | 11篇 |
2006年 | 4篇 |
2005年 | 11篇 |
2004年 | 3篇 |
2003年 | 5篇 |
2002年 | 6篇 |
2001年 | 10篇 |
2000年 | 7篇 |
1999年 | 7篇 |
1998年 | 10篇 |
1997年 | 2篇 |
1996年 | 3篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1986年 | 4篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1976年 | 4篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1973年 | 4篇 |
1972年 | 1篇 |
1970年 | 1篇 |
1968年 | 3篇 |
1962年 | 1篇 |
排序方式: 共有203条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
Stephen W. Paddock 《BioEssays : news and reviews in molecular, cellular and developmental biology》1994,16(5):357-365
The laser scanning confocal microscope (LSCM) 1 LSCM: laser scanning confocal microscope; FISH: fluorescence in situ hybridisation; DiO6: 3,3′-dihexyloxacarbocyanine iodide; NBD-ceramide: 6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-caproyl)sphingosine; DiO: 3,3′-dioctadecyloxacarbocyanine perchlorate; DiI: 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indocarbocyanine perchlorate; CCD: charge-coupled device; DIC: differential interference contrast; FURA2: (-(2-(5-carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy)-2-)2′-amino-5′-methylphenoxy)-ethane-N,N,N′,N′-tetraacetic acid, sodium salt);BCECF: 2′,7′-bis-(carboxyethyl)-5-(and-6-)-carboxyfluorescein;fluo-3: 1-(2-amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)-2-(2′amino-5′-methylphenoxy)-ethane-N,N,N′,N′,-tetraacetic acid, ammonium salt; DAPI: 4′,6-diamidino-2-phenylindole, dihydrochloride; PET: positron emission tomogrophy; CT: computer-assisted tomogrophy; CiD: cubitus interruptus dominus; MRC: Medical Research Council; TOTO-1: benzothiazolium-4-quinolinium dimer; YOYO-1: benzoxazolium-4-quinolinium dimer; ex.: excitation wavelength; em.: emission wavelength. is now established as an invaluable tool in developmental biology for improved light microscope imaging of fluorescently labelled eggs, embryos and developing tissues. The universal application of the LSCM in biomedical research has stimulated improvements to the microscopes themselves and the synthesis of novel probes for imaging biological structures and physiological processes. Moreover the ability of the LSCM to produce an optical series in perfect register has made computer 3-D reconstruction and analysis of light microscope images a practical option. 相似文献
5.
6.
The nucleotide sequence of a T4 tRNA with an anticodon for glycine has been determined using 32P-labeled material from T4-infected cultures of Escherichia coli. The sequence is: pGCGGAUAUCGUAUAAUGmGDAUUACCUCAGACUUCCAAψCUGAUGAUGUGAGTψCGAUUCUCAUUAUCCGCUCCA-OH. The 74 nucleotide sequence can be arranged in the classic cloverleaf pattern for tRNAs. The anticodon of T4 tRNAGly is UCC with a possible modification of the U. The tRNA molecule would thus be expected to recognize the glycine codons GGG and GGA. Comparative analysis of tRNAsGly from T2 and T6 indicate that their sequences are identical with that from T4. 相似文献
7.
Huang , P. C. (California Inst. Technol., Pasadena), and E. F. Paddock . The time and site of the semidominant lethal action of “Wo” in Lycopersicon esculentum. Amer. Jour. Bot. 49(4): 388–393. Illus. 1962.—The semidominant lethal gene, Wo (Woolly), in Lycopersicon esculentum has been investigated with specific attention to its lethal action. The primary lethal action is believed to occur prior to the torpedo stage of embryonic development (approximately 22 days after anthesis). The presumed WoWo embryos cease to grow before any cambial differentiation or organogenesis can be recognized. Their accompanying endospermous cells, however, continue to divide, this resulting in full-sized seeds. The death of these embryos probably occurs near the time of maturation of the seeds (approximately 52 days after anthesis or when the ovulary turns orange-red). The mechanism of death of the embryo apparently lies in and is specific to the embryo; hence, this is a case of autogenously determined embryonic lethality. 相似文献
8.
9.
10.
Paddock S 《BioTechniques》2001,30(4):756-7, 760, 763