Culture of Mesophyll Protoplasts and Stem Segments of Antirrhinum majus (Snapdragon): Growth and Organization of Embryoids

Protoplasts isolated enzymatically from the leaves of Antirrhinummajus L. were cultured on a synthetic medium and their growthand development were followed in vitro. Cultured protoplastsincreased in size, regenerated cell walls, divided and formedsmall colonies and embryoids. Cell wall formation and cell divisionrequired both auxin and cytokinin in the medium. Isolated stemsegments of the same species, when cu1tured on a synthetic mediumwith an added auxin, yielded rapidly growing callus tissuesin which differentiation of embryoids occurred.