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排序方式: 共有144条查询结果,搜索用时 15 毫秒
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Glucagon Affinity Absorbents: Selective Binding of Receptors of Liver Cell Membranes 总被引:3,自引:0,他引:3
AFFINITY chromatography has been used in the rapid isolation of enzymes, antibodies, antigens and other ligand-binding proteins1–6. Selective adsorbents with biological specificity perhaps may best be used in the resolution and isolation of complex biological structures and important regulatory macromolecules present in cells in very low amounts. For example, polypeptide and steroid hormone receptors, drug receptors, transport proteins and repressor molecules may be well suited for study by this technique because they display specific binding functions with a high degree of affinity. 相似文献
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Eight polymorphic microsatellites were developed in the perennial herbaceous Aster amellus L. (Asteraceae) and characterized on three populations from France and Switzerland. The number of alleles ranged between four and 30 depending on the locus, and the mean number of effective alleles was 5.8. The average gene diversity equalled 0.744 (range: 0.419–0.957) and the overall differentiation was found significant (θ = 0.092, P < 0.01). Three loci displayed significant heterozygote deficiencies, which might indicate the presence of null alleles. Amplifications were detected on eight loci in Aster alpinus L. 相似文献
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LOUWRANCE P. WRIGHT BRENDA D. WINGFIELD PEDRO W. CROUS MICHAEL J. WINGFIELD 《Molecular ecology resources》2007,7(2):343-345
Ten polymorphic microsatellite markers were developed for Cylindrocladium pauciramosum, a plant pathogen with a wide host range, which poses a serious problem in South African Eucalyptus nurseries. Polymorphism was evaluated on 43 isolates collected from Colombia and South Africa. Each locus had between three and six alleles. Testing for random mating showed multilocus equilibrium for a population of 40 isolates from a South African forestry nursery. Cross‐species transferability tested for 19 other Cylindrocladium species found amplification only in C. spathulatum, which is phylogenetically closely related to C. pauciramosum. 相似文献
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ANNA C. D. R. BARBOSA THAÍS C. CORRÊA FELIPE GALZERANI PEDRO M. GALETTI TERUMI HATANAKA 《Molecular ecology resources》2006,6(3):936-938
Prochilodus species inhabit the main river systems of South America and usually present commercial value to inland fishing. In the present study, we describe the isolation and characterization of 13 novel microsatellite loci in Prochilodus argenteus. The number of alleles per polymorphic locus varied from four to 22 and the observed heterozygosity ranged from 0.333 to 0.893. Additionally, cross‐species amplification was successful in two other Prochilodus species. These loci will be useful for studies of the population genetic structure in this fish group. 相似文献
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LOUWRANCE P. WRIGHT BRENDA D. WINGFIELD PEDRO W. CROUS TIM BRENNEMAN MICHAEL J. WINGFIELD 《Molecular ecology resources》2006,6(1):110-112
Twelve polymorphic microsatellite markers were developed for Cylindrocladium parasiticum, a plant pathogen with a wide host range and the causal agent of the serious disease of peanuts (Arachis hypogaea) known as cylindrocladium black rot (CBR). Polymorphism was evaluated on 17 isolates from different hosts and regions. Each locus had between two and six alleles. Cross‐species transferability tested for 20 other Cylindrocladium species found amplification only in Cylindrocladium pacificum, which is phylogenetically closely related to C. parasiticum. 相似文献
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We describe seven microsatellite loci, including tetra‐, tri‐ and dinucleotides, isolated from Brycon hilarii, which is a migratory fish inhabiting the Paraguay River basin (Brazil) and is highly valued for its meat quality. Three to eight alleles per locus were detected and the expected heterozygosity ranged from 0.31 to 0.81. Positive results were obtained with cross‐amplification in five other species of Brycon. These microsatellites provide a potential tool for wild population and aquaculture studies of B. hilarii and other species of the genus. 相似文献
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Algal biochar: effects and applications 总被引:1,自引:0,他引:1
MICHAEL I. BIRD CHRISTOPHER M. WURSTER PEDRO H. DE PAULA SILVA NICHOLAS A. PAUL ROCKY DE NYS 《Global Change Biology Bioenergy》2012,4(1):61-69
Algae represent a promising target for the generation of bioenergy through slow pyrolysis, leading to the production of biochar. This study reports experiments conducted on the production of freshwater and saltwater macroalgal biochar in pilot‐scale quantities, the physical and chemical characteristics of the biochars, and their impact on plant growth. The biochars are low in carbon (C) content, surface area and cation exchange capacity, while being high in ash and nutrients. Trace element analysis demonstrates that macroalgal biochar produced from unpolluted water does not contain toxic trace elements in excess of levels mandated for unrestricted use as a biosolids amendment to soils. Pot trials conducted using a C and nutrient‐poor soil, without and with additional fertilizer, demonstrate dramatic increases between 15 and 32 times, respectively, in plant growth rate for biochar treatments compared with the no biochar controls, with additional smaller increases when fertilizer was added. Pot trials conducted using a relatively fertile agricultural soil showed smaller but significant impacts of biochar amendment over the controls. 相似文献