Production of extracellular debranching activity by amylolytic yeasts

Summary The production of extracellular pullulan-degrading enzymes by several amylolytic yeast strains was studied. The highest activity was obtained with species of Endomycopsis, Lipomyces, Filobasidium, Leucosporidium, and Trichosporon, and also with some amylase-hyperproducing mutants. The most active strains are potentially valuable partners for intergeneric protoplast fusion.     

Effect of medium composition on amylase production by some starch-degrading yeasts

Abstract The production of extracellular amylase activity by a number of recently described amyloytic yeast species, viz. Candida homilentoma, C. silvanorum, C. tsukubaensis, Cryptococcus flavus, Leucosporidium capsuligenum, Filobasidium capsuligenum and Trichosporon pullulans , was investigated. The effects on amylase secretion of pH, different carbon sources (glucose, maltose, dextrin, soluble starch) and of various nitrogen sources [yeast nitrogen base, yeast extract, corn steep liquor (CSL)] were compared for these yeasts.