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The effect of diethyl pyrocarbonate on chromatophores and isolated pigment--protein complexes of Chromatium minutissimum was studied. It is shown that modification of histidine residues results in the destruction of the core antenna LHI (B880) and in a spectral shift from 850 to 830 nm in the peripheral antenna LHII (B800-850). In the purple sulfur bacterium Chromatium minutissimum the pigment--protein complexes B800-B850 (peripheral antenna, LHII) and B880 (core antenna, LHI) collect and transmit the absorbed light energy to the reaction centers. The composition of pigments and proteins as well as primary structure of the majority of polypeptides in both types of complexes from various photosynthetic bacteria have been determined. 相似文献
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Taher A Shammaa D Bazarbachi A Itani D Zaatari G Greige L Otrock ZK Mahfouz RA 《Molecular biology reports》2009,36(6):1555-1557
JAK2 is a cytoplasmic tyrosine kinase that has a vital role in signal transduction from several hemopoietic growth factor
receptors. The JAK2 V617F mutation has been implicated in a variety of diseases mainly related to myeloproliferative disorders including polycythemia
Vera, essential thrombocythemia, and idiopathic Myelofibrosis but has not been previously described in Thalassemia patients.
We studied 36 Lebanese patients diagnosed with thalassemia intermedia and assessed the presence or absence of the JAK2 V617F mutation using JAK2 activating mutation assay (In VivoScribe Technologies) and Polymerase Chain Reaction (PCR). None of the thalassemia intermedia patients were positive for this mutation.
To our knowledge, this study is the first to determine the status of JAK2 V617F mutation in thalassemia intermedia patients and expands the international published literature on JAK2. The latter’s V617F mutation does not seem to play a role in this hematologically important clinical entity. 相似文献
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We studied the distribution of the D/D, I/D, and I/I genotypes of the angiotensin-converting enzyme (ACE) in a sample of healthy Lebanese individuals to assess their prevalence
and compare them with other populations. ACE genotypes were determined using the Cardiovascular Disease (CVD) StripAssay,
which is based on a Polymerase Chain Reaction-Reverse hybridization technique. DNA from 133 unrelated healthy donors from
our HLA-bank was used. The prevalence of D/D, I/D, and I/I genotypes was found to be 39.1, 45.1, and 15.8% respectively, with D and I allelic frequency of 61.7 and 38.3%, respectively. The sampled Lebanese population showed ACE genotypic distributions similar
to Caucasians; however, with tendency towards harboring high D allele frequency together with a low I allele frequency just like the Spanish population. This first report from Lebanon will serve as a baseline statistical data
for future investigations of the prevalence of ACE genotypes in association with various clinical entities notably cardiovascular
diseases. The medical literature was also reviewed in this context. 相似文献
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Mahfouz RA Sabbagh AS Zahed LF Mahfoud ZR Kalmoni RF Otrock ZK Taher AT Zaatari GS 《Molecular biology reports》2006,33(2):145-149
Apolipoprotein E (ApoE) genotypes were studied in order to determine the prevalence in the Lebanese population and compare
it with other populations. DNA from 160 unrelated healthy donors from our HLA-bank was used. ApoE genotype was determined
using the CardioVascular Disease (CVD) StripAssay (this assay is based on a Polymerase Chain Reaction-Reverse Hybridization
technique). The prevalence of genotypes E3/3, E3/4, and E2/3 was found to be 69%, 26%, and 22%, respectively, and 0.6% for
each of E2/4 and E4/4 genotypes. The Lebanese population tested showed similarities to earlier reported ApoE genotypic distributions
(high E3 allele frequency) but also peculiar differences especially to some Arabic countries (total absence of E2 allele among
Saudis) and other populations. This is the first report from Lebanon that will serve as a template for future investigations
of the prevalence of ApoE alleles in association with various clinical entities. 相似文献
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Mahfouz RA Sabbagh AS Shammaa DM Otrock ZK Zaatari GS Taher AT 《Molecular biology reports》2008,35(3):375-378
We studied the distribution of the Factor XIII gene V34L polymorphism in a sample of healthy Lebanese individuals to assess its prevalence and compare it with other populations. Factor XIII genotypes were determined using the Cardiovascular Disease (CVD) StripAssay (ViennaLab, Austria), which is based on a Polymerase Chain Reaction-Reverse hybridization technique. DNA from 205 unrelated healthy donors from our HLA database was used. The prevalence of Wild type, heterozygous, and homozygous genotypes was found to be 74.2%, 22.4%, and 3.4% respectively. The sampled Lebanese population showed that the prevalence of V34L carriers (25.8%) was lower than Caucasians in general (44.3%) and, interestingly, with a low allele frequency of 0.14 similar to that in Blacks and South Asians. This first report from Lebanon sheds light on an additional unique genetic feature of this population and will prospectively serve as a baseline statistical data for future investigations of the prevalence of Factor XIII V34L mutation in association with various clinical entities notably cardiovascular diseases. 相似文献
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Roy Khalaf Rouba Hoteit Soha Yazbek Nady El Hajj Zaher Otrock Sarah Khansa Amira Sabbagh Dina Shammaa Rami Mahfouz 《Gene》2013
Aims
The Natural Killer Cell Immunoglobulin-like Receptor (KIR) genotype profiling in Follicular Lymphoma has not been reported before in the literature.Materials and methods
DNA extracted from 20 Follicular Lymphoma patients and 62 healthy controls was analyzed for KIR genotyping using a polymerase chain reaction/sequence specific primers technique (PCR/SSP) for the presence of 16 KIR gene and pseudogene loci.Results
The AA, AB, and BB genotype frequencies were, respectively, 20%, 60% and 20% with an A:B ratio of 1:1. KIR 2DL4, KIR 3DL2, KIR 3DL3, and KIR 3DP1*003 were presented in all individuals. No significant difference between patients and controls was detected.Conclusion
KIR genotyping profile does not seem to be associated with Follicular Lymphoma. The results presented in this pilot research represent the first international report about this important clinical entity. 相似文献8.
This article mainly reviews hypercoagulability—and specifically inherited thrombophilia—in different types of surgery including
kidney transplantation, simultaneous kidney and pancreas transplantation, orthopedic surgery, vascular surgery, cardiac surgery
and other categories of surgical procedures, with a major focus on its associated complications and the need to screen or
not. A search was conducted using Medline and cross-referencing for articles related to thrombophilia, screening for it, and
its association with surgical complications post-op. Thrombosis associated predisposing entities like factor V Leiden, Prothrombin
and Methylene tetrahydrofolate reductase gene mutations, in addition to Protein C and S deficiencies, are discussed. In addition,
common and relatively uncommon complications of thrombophilia in the above mentioned surgeries will be covered in length,
as well as the necessity to screen for thrombophilia prior to the surgical step. This topic is gaining more and more importance
for both surgeons and internists (especially Hematologists) and we, herein, present a general review of the published literature
as an update on the subject. 相似文献
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Sabbagh AS Daher RT Otrock ZK Khalek RN Zaatari GS Mahfouz RA 《Molecular biology reports》2007,34(4):267-270
An interesting mutation affecting the Apo-B gene, R3500Q, is known to display variable geographical distribution in the world and is mostly implicated in the pathogenesis of Familial
Hypercholesterolemia (FH). The aim of this study is to determine the prevalence of this mutation in the Lebanese population
and compare it to the available international literature. DNA from 160 unrelated healthy donors from our HLA-bank was used
and the ApoB genotype was determined using the CardioVascular Disease (CVD) StripAssay (this assay is based on a Polymerase
Chain Reaction-Reverse Hybridization technique). The R3500Q mutation was not observed in the general Lebanese population. Since the mutation frequency is elevated in Central Europe
and tends to decrease as one moves east and south, it disappears completely in the Mediterranean regions such as Spain, Turkey
and Israel; therefore, it is rather expected to be absent in Lebanon as well. Our report adds a valuable piece of information
regarding this mutation in an Arab country and paves the way for future research involving patients diagnosed with FH in order
to assess the role of the R3500Q mutation in the development of this clinical entity. 相似文献
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