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The anthrax lethal factor is a zinc metalloprotease toxin secreted by Bacillus anthracis which cleaves at the N-terminal region of six mitogen activated protein kinase kinases (MEKs) in the cell. Additionally, it is known to cleave a nine residue peptide "LF10," 50-fold more efficiently than nine residues of MEK1. There is very little sequence similarity between the MEK N-termini, thus, it is unclear how the lethal factor can accommodate and cleave the diverse N-termini of the MEKs and whether there is a hierarchy in this interaction, as there is between LF10 and MEK1. To investigate this problem, we carried out multiple molecular dynamics simulations of the lethal factor with nine residues of each of the substrates. Our simulations reveal that like LF10, certain MEK substrates have residue compositions that favor beta-sheet formation with the lethal factor over others. The formation of this secondary structure maintains a catalytic conformation. Binding energetics using the MM-PBSA method was used to rank-order the substrates for their affinity to LF (K(M)). On the basis of the results, we conclude that the LF does not equally accommodate the MEK substrates and further predict that there will be differences between rates of cleavage among the nine residue MEK N-termini. 相似文献
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柯壮 Osagie Obamwonyi Boris Kolvenbach 季荣 刘双江 蒋建东 Philippe F.-X. Corvini 《生物工程学报》2021,37(10):3475-3486
排放到环境中的各种农药、多环芳烃、卤代芳烃等有机污染物以及阻燃剂等新兴污染物,对环境污染、农产品质量和环境安全造成了沉重负担。因此,有效去除环境中的有机污染物已成为迫在眉睫的挑战。3D生物打印技术已经在医学材料、制药等行业中发挥着重要作用。现在,越来越多的微生物被确定适合通过3D生物打印生产具有复杂结构和功能的生物材料。微生物的3D生物打印越来越受到环境微生物学家和生物技术专家的关注。本文综述了用于污染物微生物去除的不同3D生物打印技术的原理和优缺点,及用于微生物生物修复技术的可行性,并指出了可能遇到的限制和挑战。 相似文献
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The Oomycete Saprolegnia ferax incorporates 3H from both cycloartenol-[2-3H] and lanosterol-[2-3H] into its normal sterols cholesterol, fucosterol, desmosterol, and 24-methylenecholesterol. It is concluded that sterol biosynthesis in this organism is via cycloartenol and the taxonomic implications are discussed. 相似文献
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Okoh Love Ajayi Abayomi M. Ben-Azu Benneth Akinluyi Elizabeth T. Emokpae Osagie Umukoro Solomon 《Molecular biology reports》2020,47(10):7709-7722
Molecular Biology Reports - Adaptogens are substances that act nonspecifically to combat stress by regulating the key elements involved in stress-induced pathologies. d-Ribose–l-cysteine... 相似文献
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Oshodi Tolulope Olabode Ben-Azu Benneth Ishola Ismail O. Ajayi Abayomi Mayowa Emokpae Osagie Umukoro Solomon 《Molecular biology reports》2021,48(3):2335-2350
Molecular Biology Reports - Mounting evidences have shown that nicotinamide adenine dinucleotide phosphate oxidase-2 (Nox-2) pathway modifies glutamic-acid decarboxylase-67 (GAD67) (GABAergic... 相似文献
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