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S. H. Habib S.-E. Ooi Ond?ej Novák Danu?e Tarkowská Jakub Rol?ík Karel Dole?al S. S. R. Syed-Alwee C.-L. Ho P. Namasivayam 《Plant Growth Regulation》2012,68(2):313-317
Comparative mineral and hormonal analyses were made on tissue culture derived truncated leaf syndrome and wild type oil palm seedlings. Mineral analysis confirmed that Boron, Zinc and chlorophyll levels were significantly lower in truncated leaf syndrome leaves than those of wild type. Hormonal analysis also revealed various cytokinin derivatives such as trans-zeatin, trans-zeatin riboside, trans-zeatin O-glucoside and trans-zeatin riboside 5??mono phosphate were significantly higher in truncated leaf syndrome leaves compared to wild type leaves. Brassinolide level was also significantly higher in truncated leaf syndrome leaves than those of the wild type. These observations suggest that the truncated leaf syndrome abnormality could be associated to high cytokinin and brassinosteroid production which affects the uptake of Boron and Zinc. 相似文献
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Differential tolerance ofArabidopsis thaliana crown gall tumors and calli to 5-bromo-2′-deoxyuridine
M. Ondřej 《Biologia Plantarum》1983,25(5):391-393
The degree of tolerance of two crown gall tumors and leaf calli ofArabidopsis thaliana to BUdR was compared. The nopaline producing teratoma tumor tolerated BUdR in concentration as high as 2.10?4 M. The tolerance of octopine producing unorganized crown gall tumor to BUdR was lower, but both exceeded significantly the degree of tolerance to BUdR of untransformedA. thaliana calli, where 10?5 M BUdR already show some inhibitory effect on the growth rate. 相似文献
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Barley embryos were treated by 0.1% colchicine for 30 min. Samples of root tips were fixed after 4 h, 8 h and 12 h. In the first sample,c-metaphases, normal metaphases and anaphases were present jointly. Inc-metaphases, chromosomes sometimes tended to make two groups with 7 chromosomes in each. In anaphases, lagging chromosomes, tripolar and multipolar anaphasos were found. No chromosomal aberrations were detected in anaphases and metaphases. No chromosome disturbances were found in root tips sampled 8 h and 12 h after colchicine treatment. 相似文献
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The influence of pretreatment with exogenous DNA on the frequencies of aberrations induced by X-Rays
Pretreatment with calf thymus DNA decreases the frequency of chromosomal aberrations in metaphases and anaphases induced by X-rays in barley root meristem cella. This effect is manifested in a wide range of concentrations (5% -0.001%) without any great dependence on the concentration. Calf thymus DNA alone does not induce any chromosomal aberrations in barley root meristem cells. 相似文献
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Na+,K+/H+ antiporters regulate the pH of endoplasmic reticulum and auxin‐mediated development 下载免费PDF全文
Ligang Fan Lei Zhao Wei Hu Weina Li Ondřej Novák Miroslav Strnad Sibu Simon Jiří Friml Jinbo Shen Liwen Jiang Quan‐Sheng Qiu 《Plant, cell & environment》2018,41(4):850-864
AtNHX5 and AtNHX6 are endosomal Na+,K+/H+ antiporters that are critical for growth and development in Arabidopsis, but the mechanism behind their action remains unknown. Here, we report that AtNHX5 and AtNHX6, functioning as H+ leak, control auxin homeostasis and auxin‐mediated development. We found that nhx5 nhx6 exhibited growth variations of auxin‐related defects. We further showed that nhx5 nhx6 was affected in auxin homeostasis. Genetic analysis showed that AtNHX5 and AtNHX6 were required for the function of the endoplasmic reticulum (ER)‐localized auxin transporter PIN5. Although AtNHX5 and AtNHX6 were colocalized with PIN5 at ER, they did not interact directly. Instead, the conserved acidic residues in AtNHX5 and AtNHX6, which are essential for exchange activity, were required for PIN5 function. AtNHX5 and AtNHX6 regulated the pH in ER. Overall, AtNHX5 and AtNHX6 may regulate auxin transport across the ER via the pH gradient created by their transport activity. H+‐leak pathway provides a fine‐tuning mechanism that controls cellular auxin fluxes. 相似文献
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Steroidal compounds have been utilized as carriers and for modification of physico-chemical properties of model biologically active secondary alcohols - juvenoids. Juvenoids are juvenile hormone analogues - environmentally safe insecticides, possessing significant biological activity towards different arthropods groups in focus on insect pest species. Structure modification of juvenoids plays important role to control the rate of liberation and decomposition of juvenoid in digestive system and can also play important role in the mode of action towards selected insect. This study presents an approach to the synthesis of steroidal monomers and dimers carrying one and two molecules of a juvenoid in their structures. The prepared compounds were tested for their inhibition activity on reproduction of the blowfly Neobellieria (Sarcophaga) bullata. These steroid-juvenoid conjugates showed promising possibilities in synthesis of new unique biochemical insecticides. Preliminary biological test results of prepared compounds are presented. 相似文献