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Root rot of pawpaw (Carica papaya L.) reported in Nigeria is caused byPythium aphanidermatum which was consistently isolated from diseased plant parts and highly pothogenic. Out of 16 different media tested, it grew best on corn-meal-agar (CMA) and CMA supplemented with cellulose and sucrose. The highest number of oospores/ml was on CMA with average diameter of 19.9±0.1 µm. The symptom is characterized by dark brown rot of roots, absence of secondary roots and disintegration of internal tissue of the main root. These cause the progressive decline of the aerial parts of the tree untill it dies. 相似文献
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在疫霉属真菌中,很多种是重要的经济植物的病原菌,寄主范围广泛,包括乔木、灌木和各种农作物;为害性大,常带来严重的经济损失。本文主要研究疫霉异宗配合种的交配型。根据疫霉在纯培养和成对培养(dual culture)中产生有性器官的能力,疫霉属可分为同宗配合种和异宗配合种两大类群。异宗配合种的A~1交配型与同一种或其它种的A~2交配型进行成对培养时,可以形成有性器官。两个可亲合菌系配合而形成有性器官时,可能发生基因重组,其结果将使病原菌具有更强的生存能力、致病力以及更广泛的寄主范围。因此,研究疫霉两种不同的交配型的分布,不仅对认识病害的发生发展规律,进一步设计防治措施有着重要意义,而且对疫霉属的起源、演化和移栖也有着深远的理论意义。作者对收集到的7个异宗配合种:Phytophthora capsici,P.cinnamomi,P.citrophthora,P.colocasiae,P.infestans,P.nicotianae,P.palmivora的38个分离物进行了交配型的研究,测定工作使用澄清的Campbell蔬菜汁琼脂培养基(V8C),用于确定交配型的菌系有P.nicotianae var.parasitica A~1,P.nicotianae var.parasitica A~2,P.cinnamomi A~1,P.cinnamomi A~2,P.palmivora A~1,P.palmivora A~2.每个分离物分别与已知种的A~1和A~2两个菌系成对接种于同一V8C平板上,放入25℃温箱中培养,2周后在两个菌落的连线上检查有性器官的产生情况。实验结果表明,中国疫霉属异宗配合种的这些分离物的交配型与寄主或地理分布似无相关性,同一种植物上分离到的同种疫霉可以是A~1交配型,也可以为A~2交配型;同一地区可以出现两种交配型,不同地区又有相同的交配型。云南西双版纳橡胶园中的分离物(P.citrophthora,P.colocasiae,P.palmivora)都表现为中性。 相似文献
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影响蛾类趋光性和灯光诱蛾效果的因素很多。过去,国内外许多观察和实验都侧重在研究光源的辐射光谱和辐射强度以及气候条件的影响。至于蛾类的主要感光器官的适应状态怎样影响趋光性,却极少引起注意。 陈元光等(1963)曾经用电生理学的方法,测出粘虫蛾复眼的暗适应过程大约为30—45分钟。Agee(1972)用电生理方法研究复眼,发现美国棉铃虫复眼在暗适应中,达到最高灵敏度的时间为30分至285分,雌、雄的平均时间分别为88分和115分;美国烟青虫雌、雄蛾的平均时间分别为119分和121分。最近,李典谟等(1977)以复眼反射光的增强作为暗适应的指标,测出烟青虫蛾在暗室内暗适应时,复眼的转化速率很不一致。快的个体约经过1小时就达到最大反射值;慢的个体则需经过3小时,而绝大多数个体(79%)则在1—2(1/2)小时内。因此,他们推测在入夜后的一段时间里,田间昆虫的眼由昼眼转化为夜眼的情况是各不相同的,并且认为这种差异会影响成虫对于灯光发生不同的行为反应。棉铃虫(Heliothis armigera)蛾是灯光防治的主要对象之一,为了进一步探索这个问题,我们认为有必要检查一下在日夜交替的自然暗适应条件下其复眼状态究竟是否一致,复眼状态怎样影响着趋光性,以便深入地了解夜蛾类趋光性的控制因素。 相似文献
5.
在31只氯醛糖和氨基甲酸乙酯麻醉的猫,观察了选择性激活颈动脉压力和化学感受器对巨细胞旁外侧核(PGL)单位放电的影响。136个PGL自发放电单位中,有84个在激活颈动脉压力感受器(BA)(新福林,1—2μg/kg,iv)和/或激活颈动脉化学感受器(CA)(nicotine 5—20μg,溶于0.25—0.5ml生理盐水中,注入甲状腺动脉)时,放电频率有变化。在这些有反应的单位中,16个仅对CA起反应(11个兴奋、5个抑制);54个以各种组合方式对CA和BA都起反应,其中以CA引起兴奋反应而BA引起抑制反应的占比例最大;14个仅对BA起反应(7个兴奋,7个抑制)。在定位分布上,那些只对CA起反应的单位多位于PGL的腹侧部份;仅对BA起反应的单位则位于对CA起反应单位的较背侧;对BA和CA均起反应的单位介于上述两者之间或在较深区域。这些结果表明,颈动脉区压力和化学感受器活动传入到PGL,并会聚在其中一些神经元上。 在PGL内全部有反应的单位中,68个对激活颈动脉压力感受器起反应,其中兴奋的29个,抑制的39个(P>0.05);70个对激活颈动脉化学感受器起反应,其中48个兴奋,22个抑制(P<0.005)。这些结果提示,BA对PGL神经元引起兴奋和抑制两种效应,而CA则诱发兴奋为主的反应。 相似文献
6.
心房钠尿因子对麻醉家兔局部血流的影响 总被引:1,自引:2,他引:1
在42只麻醉家兔,观察了静脉注射心房肽Ⅱ(AtriopeptinⅡ,APⅡ)对局部血流量以及动脉内注射 AP Ⅱ 对局部血管阻力的影响。结果如下:(1)静脉注射 APⅡ(30μg/kg)5min后,平均动脉压(MAP)降低11.0±1.5mmHg(n=8,M±SE,下同),与溶剂对照组相比有明显差异(P相似文献
7.
Selected commercial and technical grade pesticides were tested against the egg, preparasite and adult stages of Agamermis unka , a nematode parasite of the brown planthopper, Nilaparvata lugens . The commercial insecticide, diazinon (LC = 0.37 ppm), was most toxic to the 50 preparasites, followed by phenthoate (LC = 0.43 ppm), BPMC (LC = 0.44 ppm), IBP 50 50 (LC = 0.46 ppm), cartap hydrochloride (LC = 0.82 ppm) and buprofezin + isoprocarb 50 50 (LC = 1.11 ppm). The least toxic commercial pesticide tested was the fungicide, pencycuron 50 (LC = 2.19 ppm). Out of 12 technical grade insecticides tested, phenthoate, monocrotophos, 50 diazinon and carbofuran (LC = 0.37-0.46 ppm) were highly toxic to the preparasites, followed by 50 buprofezin, BPMC and fenitrothion (LC = 0.74-0.86 ppm). Fenthion, etofenprox, chlorpyrifos, 50 imidacloprid and MIPC (LC = 1.11-2.19 ppm) were the technical grade insecticides least toxic 50 to the preparasites. Most preparasites survived for up to 24 h at the low insecticide concentrations (0.63 and 0.31 ppm). Preparasites that were exposed to BPMC for 24 h at concentrations as high as 5.0 ppm and survived the treatments infected brown planthopper nymphs. Four selected insecticides-chlorpyrifos, BPMC, imidacloprid and carbofuran-had significant adverse effects on A. unka egg hatching. Eggs that were in the insecticide solution for 168 h fared poorly with imidacloprid having the best survival ( > 2% of the eggs hatching at 0.04 ppm). No eggs hatched from the other insecticide treatments. Three selected insecticides, BPMC, imidacloprid and chlorpyrifos, tested against adult A. unka showed that most adults survived the exposure to the insecticides between 0.31 and 2.5 ppm. At 5.0 ppm of BPMC or chlorpyrifos none of the adults survived, whereas with imidacloprid 70% of the adults survived. Egg deposition by the surviving adults was greatly reduced in those treated with the insecticides compared with those in the controls. Imidacloprid had some negative impact on the preparasites' ability to infect BPH nymphs, but it had the least detrimental effect of the insecticides tested on preparasite survival and on the eggs and adults of A. unka . 相似文献
8.
DONG‐SEOK KIM SOOK‐YOUNG KIM SEONG‐JOON MOON JIN‐HO CHUNG KYU‐HAN KIM KWANG‐HYUN CHO KYOUNG‐CHAN PARK 《Pigment cell & melanoma research》2001,14(2):110-115
Ceramide is a bioactive sphingolipid that mediates a variety of cell functions. However, the effects of ceramide on cell growth and the melanogenesis of melanocytes are not known. In the present study, we investigated the actions of cell‐permeable ceramide and its possible role in the signaling pathway of a spontaneously immortalized mouse melanocyte cell line, Mel‐Ab. Our results show that C2‐ceramide inhibits DNA synthesis in Mel‐Ab cells and G361 human melanoma cells in a dose‐dependent manner. Cell cycle analysis confirmed the inhibition of DNA synthesis by a reduction in the S phase. To investigate the ceramide signaling pathway, we studied whether C2‐ceramide is able to influence extracellular signal‐regulated kinase (ERK) and/or Akt/protein kinase B (PKB) activation. We demonstrated that phosphorylated Akt/PKB is decreased by C2‐ceramide, whereas phosphorylated ERK was only slightly affected. Therefore, the C2‐ceramide‐induced inactivation of Akt/PKB may be closely related to the reduced cell proliferation of Mel‐Ab cells. Furthermore, we assessed the effects of C2‐ceramide on the pigmentation of Mel‐Ab cells. The results obtained showed that the melanin content of cells was significantly reduced by C2‐ceramide at concentrations in the range of 1–10 μM, and that the pigmentation‐inhibiting effect of C2‐ceramide is much greater than that of kojic acid at 1–100 μM. In addition, we found that the activity of tyrosinase is reduced by C2‐ceramide treatment. Our results demonstrate that C2‐ceramide reduces the pigmentation of Mel‐Ab cells by inhibiting tyrosinase activity. 相似文献
9.
The new names Gentiana membranulifera T. N. Ho and G. nudicaulisKurz var. assamensis T. N. Ho are proposed. The new combinations Gentiana lateriflora Hemsl. var. uncifolia (H. J. Lam) T. N. Ho, G. sumatrana Ridl.var. humifusa (S. Moore) T. N. Ho, G. quadrifaria Bl. var. wightii (Kusnez.)T. N. Ho, G. loerzingii Ridl. var. timida (Kerr) T. N. Ho, and G. membranulifera T. N. Ho var. recurvata (Kusnez.) T. N. Ho are made. 相似文献
10.