全文获取类型
收费全文 | 8502篇 |
免费 | 952篇 |
国内免费 | 2篇 |
专业分类
9456篇 |
出版年
2023年 | 46篇 |
2022年 | 75篇 |
2021年 | 193篇 |
2020年 | 113篇 |
2019年 | 137篇 |
2018年 | 170篇 |
2017年 | 160篇 |
2016年 | 235篇 |
2015年 | 381篇 |
2014年 | 416篇 |
2013年 | 466篇 |
2012年 | 641篇 |
2011年 | 636篇 |
2010年 | 399篇 |
2009年 | 335篇 |
2008年 | 486篇 |
2007年 | 485篇 |
2006年 | 415篇 |
2005年 | 398篇 |
2004年 | 424篇 |
2003年 | 368篇 |
2002年 | 331篇 |
2001年 | 140篇 |
2000年 | 107篇 |
1999年 | 123篇 |
1998年 | 86篇 |
1997年 | 77篇 |
1996年 | 56篇 |
1995年 | 61篇 |
1994年 | 48篇 |
1993年 | 49篇 |
1992年 | 93篇 |
1991年 | 83篇 |
1990年 | 61篇 |
1989年 | 72篇 |
1988年 | 60篇 |
1987年 | 49篇 |
1986年 | 60篇 |
1985年 | 61篇 |
1984年 | 63篇 |
1983年 | 49篇 |
1982年 | 37篇 |
1981年 | 37篇 |
1980年 | 39篇 |
1979年 | 42篇 |
1978年 | 44篇 |
1977年 | 45篇 |
1976年 | 33篇 |
1974年 | 35篇 |
1972年 | 33篇 |
排序方式: 共有9456条查询结果,搜索用时 8 毫秒
1.
2.
3.
Shashank Hambarde Chi-Lin Tsai Raj K. Pandita Albino Bacolla Anirban Maitra Vijay Charaka Clayton R. Hunt Rakesh Kumar Oliver Limbo Remy Le Meur Walter J. Chazin Susan E. Tsutakawa Paul Russell Katharina Schlacher Tej K. Pandita John A. Tainer 《Molecular cell》2021,81(14):2989-3006.e9
- Download : Download high-res image (222KB)
- Download : Download full-size image
4.
Danielle E. Haulsee Dewayne A. Fox Matthew W. Breece Tonya M. Clauss Matthew J. Oliver 《PloS one》2016,11(2)
We developed a long-term tagging method that can be used to understand species assemblages and social groupings associated with large marine fishes such as the Sand Tiger shark Carcharias taurus. We deployed internally implanted archival VEMCO Mobile Transceivers (VMTs; VEMCO Ltd. Nova Scotia, Canada) in 20 adult Sand Tigers, of which two tags were successfully recovered (10%). The recovered VMTs recorded 29,646 and 44,210 detections of telemetered animals respectively. To our knowledge, this is the first study to demonstrate a method for long-term (~ 1 year) archival acoustic transceiver tag implantation, retention, and recovery in a highly migratory marine fish. Results show low presumed mortality (n = 1, 5%), high VMT retention, and that non-lethal recovery after almost a year at liberty can be achieved for archival acoustic transceivers. This method can be applied to study the social interactions and behavioral ecology of large marine fishes. 相似文献
5.
Andrea Schneider Manuela Ruppert Oliver Hendrich Thomas Giang Maite Ogueta Stefanie Hampel Marvin Vollbach Ansgar Büschges Henrike Scholz 《PloS one》2012,7(12)
The decision to move towards a mating partner or a food source is essential for life. The mechanisms underlying these behaviors are not well understood. Here, we investigated the role of octopamine – the invertebrate analogue of noradrenaline – in innate olfactory attraction to ethanol. We confirmed that preference is caused via an olfactory stimulus by dissecting the function of the olfactory co-receptor Orco (formally known as OR83b). Orco function is not required for ethanol recognition per se, however it plays a role in context dependent recognition of ethanol. Odor-evoked ethanol preference requires the function of Tbh (Tyramine β hydroxalyse), the rate-limiting enzyme of octopamine synthesis. In addition, neuronal activity in a subset of octopaminergic neurons is necessary for olfactory ethanol preference. Notably, a specific neuronal activation pattern of tyraminergic/octopaminergic neurons elicit preference and is therefore sufficient to induce preference. In contrast, dopamine dependent increase in locomotor activity is not sufficient for olfactory ethanol preference. Consistent with the role of noradrenaline in mammalian drug induced rewards, we provide evidence that in adult Drosophila the octopaminergic neurotransmitter functions as a reinforcer and that the molecular dissection of the innate attraction to ethanol uncovers the basic properties of a response selection system. 相似文献
6.
Oliver Goldschmidt Jonathan F. Bard Alexan Takvorian 《Flexible Services and Manufacturing Journal》1997,9(3):251-272
Mixed-model assembly lines are becoming increasingly interesting as the use of just-in-time principles in manufacturing continues to expand. This paper presents, for the first time, complexity results for the underlying problem of product sequencing. It is shown that the problem is intractable for both the single station and the multiple station case. Nevertheless, efficient 1.5-approximation algorithms are developed for the early- and late-start models associated with the former case. Empirical results demonstrate that these algorithms perform extremely well in practice. 相似文献
7.
Frequency of Erwinia carotovora in the Alyth Burn in eastern Scotland and the sources of the bacterium 总被引:3,自引:3,他引:0
Contamination of the Alyth Burn by Erwinia carotovora was monitored monthly over 2 years at nine sites spread over a distance of ca 20 km. The bacterium was detected only once in the upper reaches of the river where it flows in uninhabited moorland but frequency of detection and contamination level tended to increase progressively as the river flowed through the middle reaches mostly in grassland to the lower reaches in arable land where the bacterium was almost always present. Erwinia populations rose from < 102 cells/1 before May to frequently > 103 but < 104 cells/l thereafter at sites in the arable land zone. A similar pattern was found in the grassland zone except that erwinia numbers were lower. Erwinia numbers at one site in the arable land zone were positively and negatively correlated with the river water temperature and flow rate respectively when there was a 1 month lag between the environmental data and the population recorded. More than 80% of isolates tested were E. carotovora subsp. carotovora.
Water from field drains in arable fields, especially those recently planted with potatoes, was frequently contaminated by E.c. carotovora , with numbers and a temporal pattern similar to those of the Alyth Burn. Drainage water from non-arable fields was rarely contaminated. Infected and rotting potatoes deposited in rivers temporarily contaminated the water. Survival of E. carotovora in dilute phosphate buffer was greater at pH 5˙7 than at pH 7˙7 and they survived for at least 10 d in river water. 相似文献
Water from field drains in arable fields, especially those recently planted with potatoes, was frequently contaminated by E.c. carotovora , with numbers and a temporal pattern similar to those of the Alyth Burn. Drainage water from non-arable fields was rarely contaminated. Infected and rotting potatoes deposited in rivers temporarily contaminated the water. Survival of E. carotovora in dilute phosphate buffer was greater at pH 5˙7 than at pH 7˙7 and they survived for at least 10 d in river water. 相似文献
8.
9.
10.
A Dutour P Giraud C Kowalski L Ouafik P Salers V Strbak C Oliver 《Biochemical and biophysical research communications》1987,146(1):354-360
The rapid changes in TRH levels in the rat pancreas during the neonatal period make this organ an interesting model for the study of the regulation of TRH biosynthesis. Pancreatic RNAs were isolated by the guanidinium thiocyanate method and layered onto CsCl cushion. Northern blot preparations were hybridized with 32P labeled TRH cDNA probe. Pancreatic TRH mRNA was first detected in 19-day old fetuses and reached the highest level on day 0, then decreased, being barely detectable 14 days after birth. The neonatal injection of streptozotocin induced a dramatic drop of TRH mRNA levels 24 hours later. This result suggests that the peculiar evolution of TRH level in pancreas is partly due to the evolution of the expression of the TRH gene. 相似文献