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Stephka G. Chankova Nadezhda P. Yurina Evgeniya G. Dimova Olga V. Ermohina Yulia P. Oleskina Maria T. Dimitrova Peter E. Bryant 《Journal of thermal biology》2009,34(7):332-336
We aimed to clarify if heat pretreatment could protect Chlamydomonas reinhardtii cells from gamma rays DNA damaging action. It was studied whether: (1) heat pretreatment could accelerate DNA DSB rejoining; (2) chloroplast chaperones (HSP70B, HSP90C) could be involved in protection from radiation-induced DNA DSB.It was obtained that heat pretreatment (37–42 °C) induced minor DNA DSB levels which might be insufficient as signals for DNA DSB repair induction. No correlation between chaperones overproduction and DNA DSB rejoining was shown. These are probably the first data that HSP70B and HSP90C do not protect DNA against radiation-induced damage in a plant model system. 相似文献
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O. V. Ermokhina G. G. Belkina Yu. P. Oleskina S. G. Fattakhov N. P. Yurina 《Applied Biochemistry and Microbiology》2009,45(5):550-554
The effects of growth regulator of the new generation—melamine salt of bis(oxymethyl)phosphine acid (melaphene)—on culture
growth, pigment and protein content, and the induction of protective chloroplastic chaperone HSP70B in Chlamydomonas reinhardtii CW15 cells were studied. Melaphene exhibited 10–30% growth inhibition at 10−9–10−2% concentration. At 10−9–10−4% of melaphene electrophoretic concentration, the pattern of cellular proteins was similar to the control. The alterations
in protein content of algae cells were detected only at 10−2% concentration. The content of chlorophyll and carotenoids in melaphene-treated cells was 17–40% lower than in the control.
Melaphene at 10−9–10−2% concentration inhibited HSP70B induction by 39–43% compared to untreated cells. The potential mechanism of melaphene effect
might involve its influence on nuclear gene expression. 相似文献
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The cellular response of three Chlorella species that differ in their temperature preferences and tolerance – Chlorella vulgaris — Antarctic, C. vulgaris strain 8/1 — thermophilic, and Chlorella kesslery — mesophilic – is analysed. 相似文献
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Oleskina Yu. P. Yurina N. P. Mel'nik S. M. Belkina G. G. Odintsova M. S. 《Russian Journal of Plant Physiology》2001,48(4):487-492
A comparative analysis of proteins from chloroplast nucleoids was performed in two higher-plant species (Pisum sativumL. andArabidopsis thalianaL.) and a green alga Chlamydomonas reinhardtiiDang. In the nucleoids of the higher plants and the alga, 26–27 proteins were detected with their mol wts ranging from 10 to more than 94 kD. In all the species tested, the distribution of nucleoid proteins by their mol wts was similar, especially between the predominant proteins with mol wts of 10 to 40 kD. Six DNA-binding proteins (12–18 kD) were detected in nucleoids fromCh. reinhardtiichloroplasts after in vivocovalent cross-linking between chloroplast DNA and proteins. Under an electron miscroscope, some regular structures resembling nucleosome-like particles of bacterial cells were revealed. 相似文献
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N P Oleskina YuPYurina T I Odintsova T A Egorov A Otto B Wittmann-Liebold M S Odintsova 《Biochemistry and molecular biology international》1999,47(5):757-763
Basic proteins were isolated from purified pea chloroplast nucleoids by acid extraction. Using RP-HPLC, the component composition of the basic proteins was studied. SDS-PAGE of major HPLC-fractions showed that the basic nucleoid proteins are heterogeneous with mol. masses of components from 17 to 30 kDa. One polypeptide with mol. mass of 28 kDa (P28) was obtained by RP-HPLC. The sequencing of three tryptic peptides of P28 (T6, T17, and T19) showed that they are homologous to the ribosomal protein L19 of Saccharomyces cerevisiae. The possible functional role of ribosomal proteins in chloroplast nucleoids is discussed. 相似文献
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