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I. G. Minkevich A. D. Satroutdinov E. G. Dedyukhina T. I. Chistyakova E. N. Kaparullina A. V. Koshelev O. N. Okunev 《World journal of microbiology & biotechnology》2006,22(11):1205-1213
The effect of temperature on the maximum specific growth rate and the cell yield was studied during cultivation of two bacterial strains (LPM-4 and Pseudomonas sp. LPM-410) on EDTA under unlimited cell growth conditions in a pH-auxostat. Both strains displayed linear dependence of reciprocal biomass yield against reciprocal specific growth rate, from which the values of rate of substrate expenditure for cell maintenance and the “maximum” yield (i.e., hypothetical yield without cell maintenance processes) were estimated. Analysis of the maximum yield values based on mass–energy balance theory suggested that oxidation of the carboxylic acid side chains of EDTA by a monooxygenase had zero or low energetic efficiency. An Arrhenius equation with different values of Arrhenius parameters within different temperature ranges gave a good fit with the temperature dependence of both growth rate and biomass yield. Specific growth rates of both strains showed a more pronounced temperature dependence than did the cell yields. A possible kinetic mechanism was suggested which might be responsible for the modes of the temperature dependences of specific growth rate and yield that were found. The mechanism is based on a hypothetical key substance governing the metabolic flows, which is formed in a zero-order reaction and destroyed in a first-order reaction, both rate constants depending on temperature according to the Arrhenius law. 相似文献
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The SalI fragment of chromosomal DNA of Bacillus subtilis carrying the gene for lysine biosynthesis and the regulatory operator region (ribO) from the riboflavin gene was cloned into Escherichia coli cells. This fragment was shown to contain the gene coding for lysine synthesizing enzyme. Localization of this gene in Bac. subtili was determined. New plasmids pLRS33 and pLRB4 were constructed using pBR322; they carry a fragment homologous to pLP102 plasmid containing the operon for riboflavin biosynthesis. 相似文献
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本文记述采自新疆的皿蛛科蜘蛛一新届——颚齿蛛属Maxillodens gen.nov.及其一新种——鞭状颚齿蛛M.flageuatus sp.nov。 相似文献
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An enzyme active toward the methyl ester of ferulic acid was isolated from the fungus Aspergillus sp. and purified to homogeneity using ion-exchange and hydrophobic chromatography. The molecular weight of the enzyme is 42 kD, and its pI is 3.7. The enzyme has a pH optimum in the range 4-6 and a temperature optimum in the range 40 to 60 degrees C. Using a number of synthetic and natural substrates, the enzyme was identified as a feruloyl esterase. The feruloyl esterase did not hydrolyze wheat straw. Ferulic acid was detected as a product of hydrolysis of wheat bran and sugar-beet pulp. Other products were also detected after sugar-beet pulp hydrolysis. 相似文献
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A. A. Skomarovsky A. V. Gusakov O. N. Okunev I. V. Solov’eva T. V. Bubnova E. G. Kondrat’eva A. P. Sinitsyn 《Applied Biochemistry and Microbiology》2005,41(2):182-184
Enzyme preparations were isolated from the culture liquid of five mutant strains of the cellulase producer Penicillium verruculosum. The hydrolytic activities of these preparations against unbleached eucalypt cellulose was compared to that of commercial preparations of Trichoderma reesei (T. longibrachiatum). In the majority of cases, P. verruculosum enzymes provided higher yields of reducing sugars (RSs) and glucose. A correlation was found between the yield of RSs and the avicelase activity of the preparations in the reaction mixture.Translated from Prikladnaya Biokhimiya i Mikrobiologiya, Vol. 41, No. 2, 2005, pp. 210–212.Original Russian Text Copyright © 2005 by Skomarovsky, Gusakov, Okunev, Soloveva, Bubnova, Kondrateva, Sinitsyn. 相似文献
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New Effective Method for Analysis of the Component Composition of Enzyme Complexes from <Emphasis Type="Italic">Trichoderma reesei</Emphasis> 总被引:1,自引:0,他引:1
Markov AV Gusakov AV Kondratyeva EG Okunev ON Bekkarevich AO Sinitsyn AP 《Biochemistry. Biokhimii?a》2005,70(6):657-663
A method for analysis of the component composition of multienzyme complexes secreted by the filamentous fungus Trichoderma reesei was developed. The method is based on chromatofocusing followed by further identification of protein fractions according to their substrate specificity and molecular characteristics of the proteins. The method allows identifying practically all known cellulases and hemicellulases of T. reesei: endoglucanase I (EG I), EG II, EG III, cellobiohydrolase I (CBH I), CBH II, xylanase I (XYL I), XYL II, beta-xylosidase, alpha-L-arabinofuranosidase, acetyl xylan esterase, mannanase, alpha-galactosidase, xyloglucanase, polygalacturonase, and exo-beta-1,3-glucosidase. The component composition of several laboratory and commercial T. reesei preparations was studied and the content of the individual enzymes in these preparations was quantified. The influence of fermentation conditions on the component composition of secreted enzyme complexes was revealed. The characteristic features of enzyme preparations obtained in "cellulase" and "xylanase" fermentation conditions are shown. 相似文献
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Semenova MV Sinitsyna OA Morozova VV Fedorova EA Gusakov AV Okunev ON Sokolova LM Koshelev AV Bubnova IuP Sinitsyn AP 《Prikladnaia biokhimiia i mikrobiologiia》2006,42(6):681-685
A new enzyme preparation of fungal pectin lyase (EC 4.2.2.10) was shown to be useful for the production of cranberry juice and clarification of apple juice in the food industry. A comparative study showed that the preparation of pectin lyase is competitive with commercial pectinase products. The molecular weight of homogeneous pectin lyase was 38 kDa. Properties of the homogeneous enzyme were studied. This enzyme was most efficient in removing highly esterified pectin. 相似文献
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V. V. Morozova M. V. Semenova T. N. Salanovich O. N. Okunev A. V. Koshelev T. V. Bubnova G. E. Krichevskii A. G. Timatkov N. V. Barysheva A. P. Sinitsyn 《Applied Biochemistry and Microbiology》2006,42(6):603-608
Commercial and pilot pectate lyase preparations (EC 4.2.2.2) have been compared. They differ in their effect on pectins with different esterification degrees (ED). The activity of the pilot preparation with respect to a substrate with ED = 70% is tenfold lower than with respect to unesterified polygalacturonic acid. For commercial preparations, this activity ratio ranged within 1.5–2. At equal pectate lyase activities, the commercial preparations better remove pectin from crude cotton fabric during its boil off. The laboratory preparation is more efficient for improving the capillarity (wettability) of the fabric owing to the cooperative effect of the pectate lyase, cellulase, and hemicellulase present in the preparation. 相似文献
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Solovyeva IV Okunev ON Kryukova EG Kochkina GA 《Systematic and applied microbiology》1999,22(4):546-550
600 different natural strains of alkali-tolerant micromycetes belonging to 4 classes, 38 genera and 119 species were tested on their ability to produce neutral and alkaline cellulases. Frequency of occurrence of neutral and alkaline cellulases producers among the studied strains was estimated. It is concluded that 72% of all tested strains produce cellulases at neutral and alkaline pH values. But the largest number of neutral and alkaline cellulases producers belong to the following classes which are tolerant to extreme environmental factors: namely Hyphomycetes (genera Acremonium, Alternaria, Cylindrocarpon, Fuzarium, Penicillium, Verticillium, Myrothecium, Ulocladium, Gliocladium), Ascomycetes (genus Chaetomium) and Coelomycetes (genera Phoma, Microsphaeropsis, Aposphaeria). 相似文献