全文获取类型
收费全文 | 831篇 |
免费 | 168篇 |
专业分类
999篇 |
出版年
2023年 | 5篇 |
2021年 | 7篇 |
2020年 | 5篇 |
2019年 | 10篇 |
2018年 | 8篇 |
2017年 | 7篇 |
2016年 | 11篇 |
2015年 | 17篇 |
2014年 | 26篇 |
2013年 | 25篇 |
2012年 | 57篇 |
2011年 | 55篇 |
2010年 | 17篇 |
2009年 | 33篇 |
2008年 | 39篇 |
2007年 | 41篇 |
2006年 | 32篇 |
2005年 | 26篇 |
2004年 | 35篇 |
2003年 | 33篇 |
2002年 | 31篇 |
2001年 | 26篇 |
2000年 | 40篇 |
1999年 | 33篇 |
1998年 | 18篇 |
1997年 | 13篇 |
1996年 | 18篇 |
1995年 | 12篇 |
1994年 | 12篇 |
1993年 | 13篇 |
1992年 | 25篇 |
1991年 | 19篇 |
1990年 | 15篇 |
1989年 | 22篇 |
1988年 | 95篇 |
1987年 | 26篇 |
1986年 | 5篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1983年 | 5篇 |
1982年 | 4篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1976年 | 4篇 |
1975年 | 4篇 |
1974年 | 7篇 |
1973年 | 5篇 |
1969年 | 9篇 |
1968年 | 3篇 |
1966年 | 4篇 |
排序方式: 共有999条查询结果,搜索用时 15 毫秒
1.
Direct selection for curing and deletion of Rhizobium plasmids using transposons carrying the Bacillus subtilis sacB gene 总被引:13,自引:0,他引:13
We have constructed derivatives of the transposon Tn5 carrying the mob site (oriT) of plasmid RP4, and an nptI-sacB-sacR cassette [Ried and Collmer, Gene 57 (1987) 239-246]. The mob site, in conjunction with the antibiotic-resistance markers carried on the transposons, allows identification of transposon inserts in cryptic plasmids by mobilisation to other strains. The sacB-sacR genes allow direct selection for the loss or curing of plasmids, because only strains which no longer contain an active sacB gene are able to grow on media containing sucrose. We have tested these transposons in four strains of Rhizobium leguminosarum and two strains of Rhizobium meliloti, and have been able to demonstrate curing of several large cryptic plasmids, and generation of large deletions in many other plasmids. This method has enabled us to show that the R. leguminosarum plasmids pRL12JI and pR1eVF39f carry auxotrophic markers, and that the plasmid pR1eVF39c carries genes which affect colony morphology. 相似文献
2.
3.
4.
L L Wheeless J S Coon C Cox A D Deitch R W de Vere White L G Koss M R Melamed M J O'Connell J E Reeder R S Weinstein 《Cytometry》1989,10(6):731-738
A Bladder Cancer Flow Cytometry Network study has been carried out aimed at identification of the sources of inter- and intralaboratory variability. Replicate "cocktail" samples containing a mixture of peripheral blood lymphocytes and an aneuploid cell line and samples of peripheral blood lymphocytes serving as a DNA reference standard were distributed to five network laboratories. The samples were stained for DNA using propidium iodide, with each laboratory using its own staining protocol. Sets of these samples were analyzed by flow cytometry to obtain cellular DNA distributions. DNA index and hyperdiploid fraction were calculated for each histogram using an automated technique. Results were evaluated by analysis of variance to identify sources of variability. Three important sources of variation were found that affect flow cytometry in general and- the transportability of flow cytometry results to routine clinical use in particular. The significant variation among laboratories that is constant across time most probably represents stable differences in instrumentation, instrument set-up, and laboratory techniques. This variation can be compensated for, if it is known and stable, to develop transportable classification criteria. The second type of variation, termed the interaction component, represents differences among laboratories that are not constant across time. Sources of this variation include inconsistency in sample preparation, staining, and analysis. The elimination of this type of variation is required for meaningful comparison of data within and among laboratories and the creation of interlaboratory data-bases. The third type of variation represents pure measurement variability and affects the sensitivity of the technique. 相似文献
5.
R-banding and nonisotopic in situ hybridization: precise localization of the human type II collagen gene (COL2A1) 总被引:17,自引:4,他引:13
Ei-ichi Takahashi Tada-aki Hori Peter O'Connell Mark Leppert Ray White 《Human genetics》1990,86(1):14-16
Summary A new mapping system, based on nonisotopic in situ hybridization combined with fluorescent staining of replicated prometaphase R-bands, is described. Replication of the bands is achieved by treatment of thymidinesynchronized cells with bromodeoxyuridine. The human COL2A1 gene was mapped to band 12q13.11–q13.12 in this manner, to illustrate the potential of the technique for improving the precision of chromosomal mapping and physical ordering of genes. 相似文献
6.
7.
8.
9.
10.