Inhibition of collagen-induced platelet aggregation by antibodies to distinct types of collagens.

Aggregation of platelets by fibrils formed from collagens type I, II and III could be inhibited by coating the fibrils with anti-collagen antibodies or Fab fragments. Similar results were obtained in a clot-retraction assay. Inhibition was achieved with stoichiometric amounts of antibodies and was specific for each type of collagen. Aggregation caused by a mixture of type-I and -III collagens could only be inhibited by a mixture of antibodies against both collagens. The data show that each interstitial collagen is capable of interacting with platelets and do not support the concept of an outstanding activity of type-III collagen.     

Modified micro suction cup/rhizobox approach for the in-situ detection of organic acids in rhizosphere soil solution

Root–soil interactions can strongly influence the soil solution chemistry in the rhizosphere. In the present study we propose a modification of the classical rhizobox/micro suction cup system to make it suitable for the collection and analysis of organic acids in the rhizosphere. In order to show the potential of the method, we tested the modified system with Lupinus albus L. as a model plant known to exude large amounts of citrate. The suction cups were installed through the transparent front plate of the rhizoboxes just after the emergence of cluster roots in order to allow optimal localized collection of soil solution. A small dead-volume allowed almost immediate stabilisation with formaldehyde of the sampled soil solutions in the collection container to prevent microbial degradation. The concentrations of organic acids were significantly larger in the rhizosphere soil solution of active cluster roots of Lupinus albus L. than in the bulk soil solution (about 400 μM of citrate versus <0.05 μM). We were able to follow the exudation process in-situ, which occurred during 2–3 days. Also the concentrations of other organic acids and inorganic anions differed between the bulk soil and the rhizosphere of cluster roots, normal roots, and nodules.     

Review and downscaling of life cycle decision support tools for the procurement of low-value products

Purpose

In this article, we analyze how environmental aspects can be derived from life cycle management instruments for procurement decisions of low-value products. For our analysis, we chose the case of operating room textiles. The review includes the life cycle management instruments: life cycle assessment, environmental labels, and management systems applied within the textile industry. We do so in order to identify the most important environmental decision criteria based on which the procurer of low-value products can decide for the most environmentally friendly option.

Methods

We conducted a systematic literature search in the relevant literature databases. We critically evaluate the identified life cycle assessment studies for sound methodology, verifiability, completeness, and actuality. Based on this review, we analyze and compare the results of the three most comprehensive studies in more detail and derive the most important environmental aspects of operating room textiles. In a second step, we extend the operational perspective via the strategic perspective, namely environmental management systems and further existing life cycle instruments such as eco-labels. We then synthesize the gathered information into a decision vector. Finally, we discuss how the gathered data can be further exploited and give suggestions for a more sophisticated assessment.

Results and discussion

The review of the existing life cycle assessments on operating room textiles showed that procurers should not base their decisions exclusively on existing life cycle assessments. In addition to problems such as methodological weakness, incompleteness, outdated data, and poor verifiability, the information provided is far too complex to prepare procurement decisions regarding low-value products. Furthermore, the results for the textiles assessed in the existing life cycle assessments are not necessarily transferrable to the textiles considered by the procurer because of restrictive assumptions. Therefore, it is necessary to downscale the available information and synthesize it in an applicable decision support tool. Our decision vector consists of the key environmental aspects water, CO₂, energy, and waste and is completed by environmental management systems, eco-labels, and the countries of origin that matters for environmental and social aspects as well.

Conclusions

The decision vector supports procurers when considering environmental aspects in procurement decisions and provides a mechanism for balancing the information between overcomplexity and oversimplification. Therefore, it should be the basis for future development of an eco-label for operating room textiles.     

The thalamocortical contribution to epilepsy

The experimental literature has dealt intensively with the cortical contribution to epilepsy. Possibly because of the direction of technological advance, much less attention has been paid to the role of other structures. A model which emphasizes the role of some of those non-cortical structures, specifically that of thalamocortical modulation of cortical excitability, is developed. Some aspects of the petit mal seizure, a seizure type considered by some investigators to involve thalamocortical mechanisms, are predicted by the model. Although the thalamocortical mechanisms under study are not the only mechanism underlying seizures, a full understanding of the phenomenology of epilepsy needs to take into account the role of subcortical modification of cortical activities in addition to other mechanisms. Gloor has described two types of epileptogenesis: type I characteristic of non-convulsive seizure and type II characteristic of convulsions. There is disagreement as to whether or not the two mechanisms represent qualitatively different phenomena. Utilizing the thalamocortical model, it can be shown that the two types of epileptogenesis are qualitatively different. Furthermore, the thalamocortical model leads to a possible explanation of clinically different profiles of antipileptic efficacy of medications.     

Control of Cell Proliferation,Organ Growth,and DNA Damage Response Operate Independently of Dephosphorylation of the Arabidopsis Cdk1 Homolog CDKA;1

Entry into mitosis is universally controlled by cyclin-dependent kinases (CDKs). A key regulatory event in metazoans and fission yeast is CDK activation by the removal of inhibitory phosphate groups in the ATP binding pocket catalyzed by Cdc25 phosphatases. In contrast with other multicellular organisms, we show here that in the flowering plant Arabidopsis thaliana, cell cycle control does not depend on sudden changes in the phosphorylation pattern of the PSTAIRE-containing Cdk1 homolog CDKA;1. Consistently, we found that neither mutants in a previously identified CDC25 candidate gene nor plants in which it is overexpressed display cell cycle defects. Inhibitory phosphorylation of CDKs is also the key event in metazoans to arrest cell cycle progression upon DNA damage. However, we show here that the DNA damage checkpoint in Arabidopsis can also operate independently of the phosphorylation of CDKA;1. These observations reveal a surprising degree of divergence in the circuitry of highly conserved core cell cycle regulators in multicellular organisms. Based on biomathematical simulations, we propose a plant-specific model of how progression through the cell cycle could be wired in Arabidopsis.