全文获取类型
收费全文 | 6629篇 |
免费 | 410篇 |
国内免费 | 7篇 |
出版年
2022年 | 30篇 |
2021年 | 44篇 |
2020年 | 30篇 |
2019年 | 43篇 |
2018年 | 66篇 |
2017年 | 66篇 |
2016年 | 104篇 |
2015年 | 147篇 |
2014年 | 180篇 |
2013年 | 345篇 |
2012年 | 292篇 |
2011年 | 321篇 |
2010年 | 198篇 |
2009年 | 197篇 |
2008年 | 309篇 |
2007年 | 313篇 |
2006年 | 308篇 |
2005年 | 355篇 |
2004年 | 310篇 |
2003年 | 356篇 |
2002年 | 314篇 |
2001年 | 211篇 |
2000年 | 237篇 |
1999年 | 202篇 |
1998年 | 101篇 |
1997年 | 99篇 |
1996年 | 76篇 |
1995年 | 71篇 |
1994年 | 68篇 |
1993年 | 73篇 |
1992年 | 144篇 |
1991年 | 131篇 |
1990年 | 120篇 |
1989年 | 125篇 |
1988年 | 109篇 |
1987年 | 79篇 |
1986年 | 86篇 |
1985年 | 65篇 |
1984年 | 85篇 |
1983年 | 67篇 |
1982年 | 65篇 |
1981年 | 47篇 |
1980年 | 30篇 |
1979年 | 50篇 |
1978年 | 44篇 |
1977年 | 38篇 |
1976年 | 42篇 |
1974年 | 37篇 |
1971年 | 30篇 |
1970年 | 31篇 |
排序方式: 共有7046条查询结果,搜索用时 15 毫秒
1.
A putative GDP–GTP exchange factor is required for development of the excretory cell in Caenorhabditis elegans 下载免费PDF全文
Norio Suzuki Matthew Buechner Kiyoji Nishiwaki David H. Hall Hiroyuki Nakanishi Yoshimi Takai Naoki Hisamoto Kunihiro Matsumoto 《EMBO reports》2001,2(6):530-535
The Caenorhabditis elegans excretory cell extends tubular processes, called canals, along the basolateral surface of the epidermis. Mutations in the exc-5 gene cause tubulocystic defects in this canal. Ultrastructural analysis suggests that exc-5 is required for the proper placement of cytoskeletal elements at the apical epithelial surface. exc-5 encodes a protein homologous to guanine nucleotide exchange factors and contains motif architecture similar to that of FGD1, which is responsible for faciogenital dysplasia. exc-5 interacts genetically with mig-2, which encodes Rho GTPase. These results suggest that EXC-5 controls the structural organization of the excretory canal by regulating Rho family GTPase activities. 相似文献
2.
3.
Hoshino Atsushi; Abe Yukihide; Saito Norio; Inagaki Yoshishige; Iida Shigeru 《Plant & cell physiology》1997,38(8):970-974
The Japanese morning glory carrying the recessive mutable speckledallele with the dominant speckled-activator bears colorlessflowers with fine and round colored spots distributed over thecorolla whereas the plant without the speckled-activator producespale yellow flowers. Previous chemical analysis has indicatedthat a mutation in the gene for flavanone 3-hydroxylase (F3H)is a likely candidate for the speckled allele. However, theF3HmRNA without sequence alteration accumulates normally inthe pale yellow flowers, indicating that the speckled alleleis neither the F3H gene nor a regulatory gene acting on theF3H gene expression. (Received April 4, 1997; Accepted June 2, 1997) 相似文献
4.
T Tobe S Minoshima S Yamase N H Choi M Tomita N Shimizu 《Cytogenetics and cell genetics》1991,57(4):193-195
SP-40,40 is a serum glycoprotein consisting of two different subunits (alpha and beta) assembled into a dimer by disulfide bonds. Northern blot hybridization, using total RNA from several cell lines, showed that SP-40,40 is expressed in glioblastoma and testicular tumor cells, as well as hepatoma cells. Spot blot hybridization of flow-sorted human chromosomes, using a SP-40,40 cDNA fragment as a probe, localized the gene for SP-40,40 to human chromosome 8. This gene has been given the designation CLI, for complement lysis inhibitor, by the Human Gene Nomenclature Committee. 相似文献
5.
An examination of four species of Cirsium disclosed the presence of two new flavonoids in C. lineare. The structure of one was 5,4′-dihydroxy-6,7,3′-trimethoxyflavone (cirsilineol) 4′-monoglucoside and the other 5,3′,4′-trihydroxy-6,7-dimethoxyflavone (cirsiliol) 4′-monoglucoside. Luteolin 7-glucoside was found in C. suffultum, and pectolinarin and linarin in C. kamtschaticum and C. pectinellum. 相似文献
6.
T Maoka A Arai M Shimizu T Matsuno 《Comparative biochemistry and physiology. B, Comparative biochemistry》1986,83(1):121-124
Racemic mixtures of (3RS, 3'RS)-zeaxanthin were separated into the three optical isomers, (3R, 3'R)-zeaxanthin (1), (3R,3'S;meso)-zeaxanthin (2) and (3S,3'S)-zeaxanthin (3), by converting to their corresponding dibenzoates and by using HPLC on an optical resolution column Sumipax OA-2000. According to this procedure, it has been shown that only (1) is isolated from higher plants, shellfish, starfish, sea squirt, sea cucumber and then examined; on the other hand (1), (2) and (3) are isolated from zeaxanthin fraction of shrimp, fish and turtle examined. This is the first isolation of enantiomeric and meso-zeaxanthin in nature. 相似文献
7.
Akira Shimizu 《Journal of Ethology》1992,10(2):85-102
Anoplius eous Yasumatsu (Hymenoptera, Pompilidae) exhibits some outstanding nesting behavior. Females excavate a unicellular or multicellular
nest in wet ground, or dig a single-celled nest in rotten wood, or build clustered mud cells in narrow spaces between walls
of such substances as wood or vinyl sheets. The latter nest-type has been unknown within the subfamily Pompilinae. Females
prepare a nest-cell before hunting, and construct it further after hunting, leaving the prey near the nest (behavior-formula:
IVPTIOC). They transport their prey backward on the ground, grasping it in their mandibles by any part of the legs, and forward
on the surface film of water or on the ground, grasping it by the middle part of the 1st or 2nd legs. Their only prey is the
adult female semi-aquatic spider,Pardosa pseudoannulata (Lycosidae). These nesting and provisioning behavior patterns are compared with those of other pompilids. The position of
the present species in the behavioral evolution of the Pompilidae is suggested. 相似文献
8.
T Nimura M Okada M Shimizu M Kawabe T Itoh M Iwasaki H Kimura T Takeuchi 《The Annals of physiological anthropology》1990,9(4):329-333
Motorcyclists who work in some offices sometimes complained of coldness, pain and numbness of upper limbs. We studied how to discriminate between vibration syndrome and local fatigue of the motorcyclists. Subjects are 42 motorcyclists of an office in Aichi prefecture. 25 of them held several letters in their left hand when they delivered the letters. They complained of coldness, pain and numbness in the left upper limbs more than in the right limbs (p less than 0.01). We think that it is the local fatigue rather than the disorder of vibration syndrome that causes such symptoms. So it is very important to recognize the existence of local fatigue in order to know how to discriminate between vibration syndrome and local fatigue of the motorcyclists. 相似文献
9.
Masanori Shinzato Mikihiro Shamoto Satoru Hosokawa Chiyuki Kaneko Akido Osada Miyuki Shimizu Asako Yoshida 《Biotechnic & histochemistry》1995,70(3):114-118
The present study shows that Langerhans cells can be differentiated from Interdigitating cells at the light microscopic level. Superficial lymph nodes and skin taken from necropsies and the lymph nodes of dermatopathic lymphadenopathy (DPL) were used for this experiment. Sections of lymph node and skin were embedded using the acetone, methyl benzoate and xylene (AMeX) method and dendritic cells were immunostained with anti S-100 protein antibody (S-100, and OKT-6 (CD1a) using the restaining method. Langerhans cells in the skin were positive for both CD1a and S-100. Dendritic cells positive for both CD1a and S-100, and dendritic cells positive for S-100, but not for CD1a were observed in superficial lymph nodes. In normal superficial lymph nodes, there were more interdigitating cells than Langerhans cells. The majority of the dendritic cells in the DPL were Langerhans cells. We conclude that the S-100 and CD1a positive cells are Langerhans cells, and the S-100 positive-CD1a negative cells are interdigitating cells. 相似文献
10.