The effect of stress hormones,UV-C,and stilbene precursors on calmodulin (CaM) and calmodulin-like gene (CML) expression in Vitis amurensis Rupr

Plant Cell, Tissue and Organ Culture (PCTOC) - Urtica dioica L. (Urticaceae), popularly known as nettle, is a medicinal plant used by the textile, food and pharmaceutical industry. The aim of the...     

The effect of explant origin and collection season on stilbene biosynthesis in cell cultures of Vitis amurensis Rupr.

Plant cell and tissue cultures are considered as a source of valuable secondary metabolites but usually produce insufficient level of the compounds, which is the limiting factor for their application in biotechnology. We obtained 18 callus cell cultures from different organs of wild grape Vitis amurensis Rupr. collected at different seasons and analyzed stilbene accumulation in combination with calli growth parameters. This analysis showed that temporal and tissue origin of the calli affected the rate of stilbene biosynthesis. Stem-derived calli accumulated higher stilbene levels and exhibited a higher expression of phenylalanine ammonia-lyase (PAL) and stilbene synthase (STS) genes than calli derived from the leaves and petioles. The highest content of stilbenes was detected in the calli initiated from grapevine stems collected in the autumn. In general, all “autumn” cell cultures contained more than 2 mg g^??1 dry wt (up to 11 mg g^??1 dry wt) and exhibited high PAL and STS genes expression in comparison with the calli initiated in the summer. The content of stilbenes in the “autumn” cell cultures were comparable to the highest stilbene contents detected in other plant sources described in the literature. Thus, selecting the most optimal explant source for cell culture establishment could be an effective approach towards developing plant cell cultures producing high stilbene levels.

     

Enhancement of Stilbene Biosynthesis in Grape-Cell Cultures by Natural Products Based on Endophytes of the Wild Grape Species Vitis amurensis RUPR.

Applied Biochemistry and Microbiology - A new approach based on endophyte products is proposed to enhance the biosynthesis of stilbenes (compounds with established health benefits) and to alter...