全文获取类型
收费全文 | 7723篇 |
免费 | 1114篇 |
出版年
2023年 | 45篇 |
2022年 | 101篇 |
2021年 | 174篇 |
2020年 | 110篇 |
2019年 | 142篇 |
2018年 | 158篇 |
2017年 | 123篇 |
2016年 | 232篇 |
2015年 | 377篇 |
2014年 | 432篇 |
2013年 | 466篇 |
2012年 | 669篇 |
2011年 | 648篇 |
2010年 | 442篇 |
2009年 | 361篇 |
2008年 | 461篇 |
2007年 | 509篇 |
2006年 | 516篇 |
2005年 | 528篇 |
2004年 | 468篇 |
2003年 | 388篇 |
2002年 | 416篇 |
2001年 | 64篇 |
2000年 | 43篇 |
1999年 | 73篇 |
1998年 | 81篇 |
1997年 | 54篇 |
1996年 | 47篇 |
1995年 | 46篇 |
1994年 | 40篇 |
1993年 | 48篇 |
1992年 | 53篇 |
1991年 | 33篇 |
1990年 | 32篇 |
1989年 | 33篇 |
1988年 | 36篇 |
1987年 | 32篇 |
1986年 | 32篇 |
1985年 | 32篇 |
1984年 | 30篇 |
1983年 | 26篇 |
1982年 | 23篇 |
1981年 | 22篇 |
1980年 | 25篇 |
1979年 | 18篇 |
1978年 | 16篇 |
1977年 | 15篇 |
1976年 | 14篇 |
1975年 | 14篇 |
1974年 | 16篇 |
排序方式: 共有8837条查询结果,搜索用时 15 毫秒
1.
Nicole LeBrasseur 《The Journal of cell biology》2003,162(6):958-959
2.
Thomas A. Worthington Shannon K. Brewer Nicole Farless Timothy B. Grabowski Mark S. Gregory 《PloS one》2014,9(5)
Habitat fragmentation and flow regulation are significant factors related to the decline and extinction of freshwater biota. Pelagic-broadcast spawning cyprinids require moving water and some length of unfragmented stream to complete their life cycle. However, it is unknown how discharge and habitat features interact at multiple spatial scales to alter the transport of semi-buoyant fish eggs. Our objective was to assess the relationship between downstream drift of semi-buoyant egg surrogates (gellan beads) and discharge and habitat complexity. We quantified transport time of a known quantity of beads using 2–3 sampling devices at each of seven locations on the North Canadian and Canadian rivers. Transport time was assessed based on median capture time (time at which 50% of beads were captured) and sampling period (time period when 2.5% and 97.5% of beads were captured). Habitat complexity was assessed by calculating width∶depth ratios at each site, and several habitat metrics determined using analyses of aerial photographs. Median time of egg capture was negatively correlated to site discharge. The temporal extent of the sampling period at each site was negatively correlated to both site discharge and habitat-patch dispersion. Our results highlight the role of discharge in driving transport times, but also indicate that higher dispersion of habitat patches relates to increased retention of beads within the river. These results could be used to target restoration activities or prioritize water use to create and maintain habitat complexity within large, fragmented river systems. 相似文献
3.
4.
5.
Differential Thermolability of Exonuclease and Endonuclease Activities of the recBC Nuclease Isolated from Thermosensitive recB and recC Mutants 总被引:15,自引:10,他引:5 下载免费PDF全文
Sidney R. Kushner 《Journal of bacteriology》1974,120(3):1219-1222
The recBC nuclease (also called exonuclease V) has been partially purified from Escherichia coli K-12 strains carrying the thermosensitive recB270, recC271, and recB270 recC271 mutations. Of the multiple activities associated with the enzyme, only the adenosine 5'-triphosphate-dependent exonucleolytic hydrolysis of duplex deoxyribonucleic acid (DNA) is abnormally thermolabile. The exo- and endonucleolytic degradation of single-stranded DNA is no more thermosensitive than that catalyzed by the wild-type enzyme. These results suggest that the defects in genetic recombination, DNA repair, and the maintenance of cell viability observed in recBC mutants in vivo result primarily from the specific loss of adenosine 5'-triphosphate-dependent exonuclease active on duplex DNA. 相似文献
6.
7.
8.
Brigitte Aupetit Alexandre Ghazi Nicole Blanchouin Ren e Toury Emmanuel Shechter Jean-Claude Legrand 《BBA》1988,936(3):325-331
In this study we have measured, under experimental conditions which maintained efficient coupling, respiratory intensity, respiratory control, oxidative phosphorylation capacity and protonmotive force. Succinate cytochrome-c reductase and cytochrome-c oxidase activities were also studied. These investigations were carried out using kidney mitochondria from cyclosporine-treated rats (in vivo studies) and from untreated rats in the presence of cyclosporine (in vitro studies). Inhibition of respiratory intensity by cyclosporine did not exceed 21.1% in vitro and 15.9% in vivo. Since there was no in vitro inhibition of succinate cytochrome-c reductase and cytochrome-c oxidase activities, the slowing of electron flow observed can be interpreted as a consequence of an effect produced by cyclosporine between cytochromes b and c1. Cyclosporine had no effect on respiratory control either in vitro or in vivo. Statistically significant inhibition of the oxidative phosphorylation was observed both in vitro (6.6%) and in vivo (12.1%). Moreover, cyclosporine did not induce any change of membrane potential either in vivo or in vitro. Our findings show that cyclosporine is neither a protonophore, nor a potassium ionophore. In cyclosporine-treated rats we noticed a decrease of protein in subcellular fraction, including the mitochondrial fraction. The role of the inhibition respiratory characteristics by cyclosporine in nephrotoxicity in vivo must take account of these two parameters: inhibition of the respiratory characteristics measured in vitro and diminution of mitochondrial protein in cyclosporine-treated rats. 相似文献
9.
Mutations in the conserved C-terminal sequence in thyroid hormone receptor dissociate hormone-dependent activation from interference with AP-1 activity. 总被引:4,自引:2,他引:2 下载免费PDF全文
10.
This article is part of a Special Issue Energy Balance. 相似文献