全文获取类型
收费全文 | 9429篇 |
免费 | 1223篇 |
国内免费 | 2篇 |
专业分类
10654篇 |
出版年
2023年 | 57篇 |
2022年 | 125篇 |
2021年 | 227篇 |
2020年 | 131篇 |
2019年 | 169篇 |
2018年 | 204篇 |
2017年 | 152篇 |
2016年 | 299篇 |
2015年 | 481篇 |
2014年 | 522篇 |
2013年 | 586篇 |
2012年 | 840篇 |
2011年 | 810篇 |
2010年 | 546篇 |
2009年 | 440篇 |
2008年 | 577篇 |
2007年 | 634篇 |
2006年 | 640篇 |
2005年 | 615篇 |
2004年 | 561篇 |
2003年 | 462篇 |
2002年 | 490篇 |
2001年 | 76篇 |
2000年 | 55篇 |
1999年 | 87篇 |
1998年 | 92篇 |
1997年 | 56篇 |
1996年 | 55篇 |
1995年 | 50篇 |
1994年 | 55篇 |
1993年 | 51篇 |
1992年 | 55篇 |
1991年 | 31篇 |
1990年 | 38篇 |
1989年 | 34篇 |
1988年 | 26篇 |
1987年 | 33篇 |
1986年 | 29篇 |
1985年 | 24篇 |
1984年 | 21篇 |
1983年 | 17篇 |
1982年 | 23篇 |
1981年 | 18篇 |
1980年 | 23篇 |
1979年 | 18篇 |
1978年 | 23篇 |
1977年 | 11篇 |
1976年 | 11篇 |
1975年 | 13篇 |
1974年 | 12篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
1.
Nicole LeBrasseur 《The Journal of cell biology》2003,162(6):958-959
2.
Thomas A. Worthington Shannon K. Brewer Nicole Farless Timothy B. Grabowski Mark S. Gregory 《PloS one》2014,9(5)
Habitat fragmentation and flow regulation are significant factors related to the decline and extinction of freshwater biota. Pelagic-broadcast spawning cyprinids require moving water and some length of unfragmented stream to complete their life cycle. However, it is unknown how discharge and habitat features interact at multiple spatial scales to alter the transport of semi-buoyant fish eggs. Our objective was to assess the relationship between downstream drift of semi-buoyant egg surrogates (gellan beads) and discharge and habitat complexity. We quantified transport time of a known quantity of beads using 2–3 sampling devices at each of seven locations on the North Canadian and Canadian rivers. Transport time was assessed based on median capture time (time at which 50% of beads were captured) and sampling period (time period when 2.5% and 97.5% of beads were captured). Habitat complexity was assessed by calculating width∶depth ratios at each site, and several habitat metrics determined using analyses of aerial photographs. Median time of egg capture was negatively correlated to site discharge. The temporal extent of the sampling period at each site was negatively correlated to both site discharge and habitat-patch dispersion. Our results highlight the role of discharge in driving transport times, but also indicate that higher dispersion of habitat patches relates to increased retention of beads within the river. These results could be used to target restoration activities or prioritize water use to create and maintain habitat complexity within large, fragmented river systems. 相似文献
3.
4.
5.
Amphotericin B (AmB) increased unidirectional Na transport and net transcellular sodium movements across the skin of the frog, Rana pipiens, when added to the solution bathing the corium side, but not from the outer epidermal surface. The AmB response was prevented with pretreatment with amiloride, ouabain and mucosal sodium substitution. Alteration in pH markedly reduced the permeability changes induced by AmB. AmB did not interfere with the increase in sodium transport induced by antidiuretic hormone. The present study demonstrates that AmB interacts with the skin of the frog, Rana pipiens, from the corium side specifically increasing transepithelial sodium transport. The increase in transport apparently occurs through the existing sodium pathway. 相似文献
6.
7.
8.
Brigitte Aupetit Alexandre Ghazi Nicole Blanchouin Ren e Toury Emmanuel Shechter Jean-Claude Legrand 《BBA》1988,936(3):325-331
In this study we have measured, under experimental conditions which maintained efficient coupling, respiratory intensity, respiratory control, oxidative phosphorylation capacity and protonmotive force. Succinate cytochrome-c reductase and cytochrome-c oxidase activities were also studied. These investigations were carried out using kidney mitochondria from cyclosporine-treated rats (in vivo studies) and from untreated rats in the presence of cyclosporine (in vitro studies). Inhibition of respiratory intensity by cyclosporine did not exceed 21.1% in vitro and 15.9% in vivo. Since there was no in vitro inhibition of succinate cytochrome-c reductase and cytochrome-c oxidase activities, the slowing of electron flow observed can be interpreted as a consequence of an effect produced by cyclosporine between cytochromes b and c1. Cyclosporine had no effect on respiratory control either in vitro or in vivo. Statistically significant inhibition of the oxidative phosphorylation was observed both in vitro (6.6%) and in vivo (12.1%). Moreover, cyclosporine did not induce any change of membrane potential either in vivo or in vitro. Our findings show that cyclosporine is neither a protonophore, nor a potassium ionophore. In cyclosporine-treated rats we noticed a decrease of protein in subcellular fraction, including the mitochondrial fraction. The role of the inhibition respiratory characteristics by cyclosporine in nephrotoxicity in vivo must take account of these two parameters: inhibition of the respiratory characteristics measured in vitro and diminution of mitochondrial protein in cyclosporine-treated rats. 相似文献
9.
This article is part of a Special Issue Energy Balance. 相似文献
10.
F. Giuliano K. Mac Kenna S. Droupy O. Rampin V. Izard G. Benoit A. Jardin 《Andrologie》1999,9(2):226-246
Seminal emission and sperm expulsion are under the control of both the sympathetic and parasympathetic outflows and also of the somatic innervation conveyed by the pudendal nerve. The 2 phases of ejaculation are reflexive with the reflexes handled at the thoraco-lumbar and sacral levels of the spinal cord. Such a spinal organization remains widely unknown. The role of various peripheral neurotransmitters has been evidenced including norepinephrine and acetylcholine and also peptidergic, purinergic i.e. ATP and nitric oxide. Stimulation of the seminal tract afferents play a crucial in the onset of ejaculatory mechanisms. Except for the dorsal nerve of the penis, there is a lack of information concerning these afferents. Several supraspinal centers i.e. hypothalamus, medial amygdala, pons and nucleus paragigantocellularis exert descending and ascending inhibitory and excitatory influences on spinal nuclei controlling emission and expulsion of sperm. Central neurotransmission responsible for this supraspinal control could involve serotonin, oxytocin and norepinephrine. In the light of the available anatomical and neurophysiological data, pathophysiological aspects of ejaculatory disorders are futher discussed. Premature ejaculation could be related to a periheral and central hypersentivity. Most of the other ejaculation abnormalities are likely mainly related to an impairment of the central mechanisms. 相似文献