全文获取类型
收费全文 | 180篇 |
免费 | 3篇 |
出版年
2022年 | 2篇 |
2021年 | 4篇 |
2019年 | 3篇 |
2018年 | 3篇 |
2017年 | 8篇 |
2016年 | 5篇 |
2015年 | 1篇 |
2014年 | 3篇 |
2013年 | 9篇 |
2012年 | 4篇 |
2011年 | 3篇 |
2010年 | 5篇 |
2009年 | 5篇 |
2008年 | 9篇 |
2007年 | 10篇 |
2006年 | 8篇 |
2005年 | 11篇 |
2004年 | 10篇 |
2003年 | 6篇 |
2002年 | 10篇 |
2001年 | 6篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1990年 | 5篇 |
1989年 | 3篇 |
1988年 | 5篇 |
1987年 | 4篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1979年 | 3篇 |
1977年 | 1篇 |
1975年 | 4篇 |
1974年 | 3篇 |
1973年 | 4篇 |
1972年 | 2篇 |
1971年 | 2篇 |
1970年 | 1篇 |
1969年 | 3篇 |
1968年 | 3篇 |
1966年 | 2篇 |
1965年 | 1篇 |
排序方式: 共有183条查询结果,搜索用时 437 毫秒
1.
Yu. V. Nesterov D. L. Teplyi 《Journal of Evolutionary Biochemistry and Physiology》2004,40(4):438-442
Analysis of parameters of metabolic function of lungs with respect to lipid metabolism was performed in rats under conditions of acute experimental emotional-nociceptive stress. Study of the stress-sensitivity of lungs in the comparative-age aspect has shown different extent of changes of several parameters of lipid metabolism in pulmonary tissue in animals of different age. The results of the study demonstrate areactivity and high stress-stability of lungs with respect to surface-active properties, free-radical processes, and lipase activity in early postnatal ontogenesis. 相似文献
2.
Functional state of the epidermal growth factor-receptor complexes during their internalization in A-431 cells.
下载免费PDF全文
![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
A Nesterov G Reshetnikova N Vinogradova N Nikolsky 《Molecular and cellular biology》1990,10(9):5011-5014
Functional state of internalized epidermal growth factor (EGF) receptor in A-431 cells has been studied. The use of photoaffinity [125I]EGF derivative allowed us to establish that inside the cell the EGF retains its connection with the receptor. With the help of polyclonal antibodies to phosphotyrosine, it has been shown that EGF-receptor complexes maintain their phosphorylated state during internalization. The internalized EGF receptor kinase as well as that localized in the plasma membrane appeared to be able to phosphorylate synthetic peptide substrate introduced into the cell. 相似文献
3.
The compartmentalization of the epidermal growth factor (EGF) receptors in A-431 cells was studied using centrifugation of the microsomal fraction of these cells in continuous Percoll gradient. The existence of an intact (non-degraded) EGF receptor in plasma membrane and endosome fraction was demonstrated by electrophoretic analysis of in vitro phosphorylated Percoll fractions. No phosphorylated receptor was revealed in lysosomal fraction by this method. The existence of non dissociated EGF-receptor complexes in intracellular compartments 30 minutes after the start of internalization was proven using a synthesized photoreactive labeled EGF derivative (125I-EGF-SANAH). The removing of pH gradient in organellar membranes by 10 mkM of monensin did not affect dissociation from its receptor. The data obtained proved the existence of non-dissociated and non-degraded EGF-receptor complexes in the endosomal compartment of A-431 cells. 相似文献
4.
A N Nesterov 《Arkhiv anatomii, gistologii i émbriologii》1988,94(5):34-42
By means of silver nitrate impregnation after V. V. Kupriianov of blood vessels it has been revealed that the blood microcirculatory bed of the borderline structures in the anterior part of the inferior mediastinum, united by their topographical proximity and common sources of blood supply, are characterized by a high concentration of microvessels, arterioles, precapillaries, capillaries, postcapillaries and venules. In each structure investigated peculiar features of the blood microcirculatory bed organization are defined. Richness in vessels and their borderline position ensure potency of transudation and resorption of liquids from the inferior mediastinum in the cases, when the mediastinal part studied becomes the reservoir for pathological exudates (at traumas, at mediastinitis). 相似文献
5.
6.
AV Shevchenko IG Budzanivska TP Shevchenko VP Polischuk D Spaar 《Archives Of Phytopathology And Plant Protection》2013,46(2):139-146
The work was focused on the investigation of possible dependencies between the development of viral infection in plants and the presence of high heavy metal concentrations in soil. Field experiments have been conducted in order to study the development of systemic tobacco mosaic virus (TMV) infection in Lycopersicon esculentum L. cv. Miliana plants under effect of separate salts of heavy metals Cu, Zn and Pb deposited in soil. As it is shown, simultaneous effect of viral infection and heavy metals in tenfold maximum permissible concentration leads to decrease of total chlorophyll content in experiment plants mainly due to the degradation of chlorophyll a. The reduction of chlorophyll concentration under the combined influence of both stress factors was more serious comparing to the separate effect of every single factor. Plants' treatment with toxic concentrations of lead and zinc leaded to slight delay in the development of systemic TMV infection together with more than twofold increase of virus content in plants that may be an evidence of synergism between these heavy metal's and virus' effects. Contrary, copper although decreased total chlorophyll content but showed protective properties and significantly reduced amount of virus in plants. 相似文献
7.
S. M. Korotkov K. V. Sobol I. V. Shemarova V. V. Furaev A. R. Shumakov V. P. Nesterov 《Biophysics》2016,61(5):733-740
The inotropic effect of Pr3+ and La3+ ions on the heart muscle of frog Rana ridibunda, as well as the influence of the ions on respiration, swelling, and the potential (ΔΨmito) on the inner membrane of Ca2+- loaded rat heart mitochondria, energized by glutamate and malate or succinate in the presence of rotenone were studied. It was found that 2 mM Pr3+ in Ringer’s solution reduces the force of spontaneous contractions and those induced by electrical stimulation in the heart; it had a negative chronotropic effect, decreasing the frequency of spontaneous contractions. Pr3+ and La3+ prevented a decrease in the 2,4-dinitrophenol (DNP)- uncoupled respiration of energized rat heart mitochondria, swelling of these organelles in salt media, and a reduction in ΔΨmito on the inner mitochondrial membrane that were induced by Ca2+ ions. Retardation by Pr3+ and La3+ ions of these calcium-induced effects may suggest that in the inner mitochondrial membrane these metals inhibit the opening of the mitochondrial permeability transition pore caused by Ca2+ overload of mitochondria. The data we obtained are important for a better understanding of the mechanisms of the damaging action of rare-earth elements on Ca2+-dependent processes in the vertebrate myocardium. 相似文献
8.
9.
Inhibition of the receptor-binding function of clathrin adaptor protein AP-2 by dominant-negative mutant mu2 subunit and its effects on endocytosis
下载免费PDF全文
![点击此处可从《The EMBO journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Although interactions between the mu2 subunit of the clathrin adaptor protein complex AP-2 and tyrosine-based internalization motifs have been implicated in the selective recruitment of cargo molecules into coated pits, the functional significance of this interaction for endocytosis of many types of membrane proteins remains unclear. To analyze the function of mu2-receptor interactions, we constructed an epitope-tagged mu2 that incorporates into AP-2 and is targeted to coated pits. Mutational analysis revealed that Asp176 and Trp421 of mu2 are involved in the interaction with internalization motifs of TGN38 and epidermal growth factor (EGF) receptor. Inducible overexpression of mutant mu2, in which these two residues were changed to alanines, resulted in metabolic replacement of endogenous mu2 in AP-2 complexes and complete abrogation of AP-2 interaction with the tyrosine-based internalization motifs. As a consequence, endocytosis of the transferrin receptor was severely impaired. In contrast, internalization of the EGF receptor was not affected. These results demonstrate the potential usefulness of the dominant-interfering approach for functional analysis of the adaptor protein family, and indicate that clathrin-mediated endocytosis may proceed in both a mu2-dependent and -independent manner. 相似文献
10.
In an acute experiment, performed in 10 dogs with application of radiological methods, zones of the vertebral part of the intercostal arteries reservoirs and extent of their anastomization with each other both in transversal and longitudinal directions have been stated. The vertebral part of the reservoir of each intercostal artery supplies with blood that half of the corresponding segment of the spinal column, which has the same name, and tissues around, and spreads to the adjoining caudal segment. 相似文献