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1.
A I Gurevich A V Mikul'skis O V Nekrasova E A Chernen'kaia 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1988,(1):33-36
RecA-independent recombinations accompanying the processes of plasmids preparation and cloning into Escherichia coli cells are induced within the short direct and inverted repeats of several types. 相似文献
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V G Korobko E F Boldyreva O V Nekrasova A Mikul'skis S A Filippov V N Dobrynin 《Bioorganicheskaia khimiia》1991,17(4):461-469
Chemical-enzymatic synthesis and cloning in Escherichia coli of double-stranded DNAs, coding for simple and complex antigenic determinants of foot-and-mouth disease virus (FMDV) strain A22, have been carried out. The simple antigenic determinants are a part of the viral coat protein VP1 (amino acid sequence 131-152 or 131-160) whereas the complex antigenic determinants comprise additionally the amino acid sequence 200-213 of VP1 linked to N-terminus of simple antigenic determinants through a tetrapeptide spacer Pro-Pro-Ser-Pro. Recombinant DNAs containing genes for antigenic determinants of FMDV fused with C-terminus of gene for human tumor necrosis factor (hrTNF) have been constructed. Expression of the hybrid genes and properties of the proteins coded were studied. All recombinant proteins were shown to interact specifically with polyclonal antibodies both against hrTNF and FMDV strain A22. The recombinant proteins produced by bacteria are perspective for study as a vaccine against FMDV. 相似文献
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Some genetical demographic characteristics of population of Ashkhabad city were studied by direct interviewing of women aged over 18 years. The influence of urbanization on the basic genetical-demographic indices was found, the indices being essentially different from those of rural population studied elsewhere. Analysis of intensity and the structure of marriage migrations showed considerable ethnic subdivision in Ashkhabad population and stability of marriage policy in an every ethnic group. The level and structure of inbreeding as well as positive marriage assortativity are of the same order as in the Turkmen rural population. 相似文献
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S. V. Shirshev I. V. Nekrasova E. G. Orlova O. L. Gorbunova 《Doklady biological sciences》2016,470(1):249-252
Both leptin and ghrelin used separately at the concentrations corresponding to trimesters II–III of pregnancy increase the number of CD56bright NK cells in mononuclear cell suspension; their combination also enhances the L-selectin expression on the surface of these cells in the culture. These hormones do not affect the production of TGF-β1, IL-17А, or IFN-γ by NK cells, and they inhibit the production of IL-10. Leptin decreses the IL-4 production by NKp46+ cells, but the presence of ghrelin abrogates this effect. 相似文献
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Nekrasova G. F. Ronzhina D. A. Maleva M. G. P'yankov V. I. 《Russian Journal of Plant Physiology》2003,50(1):57-67
Radioisotope techniques were used to compare photosynthetic CO2 fixation, activities of carboxylating enzymes, and the composition of photosynthates in 42 species of aquatic plants (emergent, floating, and submersed hydrophytes) collected from rivers Sysert' and Iset' in Sverdlovsk oblast (Russia). The submersed leaves, in comparison with the emergent and floating leaves, featured lower rates of potential photosynthesis (by 2.2 mg CO2/(dm2 h) on average), low content of the fraction I protein, and low activity of Rubisco and phosphoenolpyruvate carboxylase (PEPC). The averaged activities of Rubisco and PEPC were diminished in submersed leaves by 10 and 1 mg/(dm2 h), respectively. Different hydrophyte groups showed similar composition of assimilates accumulated after 5-min photosynthesis and did not differ in this respect from terrestrial plants. However, the incorporation of 14C into sucrose and starch in submersed leaves (30 and 9% of total labeling, respectively) was lower than in emergent and floating leaves (45 and 15%, respectively). At the same time, the incorporation of 14C into C4 acids (malate and aspartate) was 1.5 times higher in submersed leaves than in other leaf types. Analysis of leaf differentiation, the Rubisco/PEPC activity ratio, the PEPC activity, and the composition of primary photosynthates in the pulse–chase experiments revealed no evidence of the C4 effect in the submersed hydrophytes examined. The adaptation of hydatophytes to specific conditions of an aquatic environment was structurally manifested in the reduction (by a factor of 3–5) in the number of chloroplasts per 1 cm2 leaf area. This small number of chloroplasts was responsible for low photosynthetic rates in submersed leaves, although metabolic activities of individual chloroplasts were similar for all three hydrophyte groups. 相似文献
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D. A. Dolgikh A. Yu. Malyshev S. V. Salozhin O. V. Nekrasova L. E. Petrovskaya M. V. Roshchin A. A. Borodinova T. B. Feldman P. M. Balaban M. P. Kirpichnikov M. A. Ostrovsky 《Doklady. Biochemistry and biophysics》2015,465(1):424-427
Anionic channelrhodopsin slow ChloC was expressed in the culture of nerve cells and in vivo in mouse brain. We demonstrated ability of slow ChloC to suppress effectively the activity of the neuron in response to the illumination with the visible light. It has been shown for a first time that slow ChloC works equally efficiently in both neuronal culture and in the whole brain being expressed in vivo. Thus, slow ChloC could be considered as an effective optogenetic tool capable in response to light stimulation to inhibit the generation of action potentials in the neuron. 相似文献