排序方式: 共有13条查询结果,搜索用时 15 毫秒
1.
A. D. Neklyudov A. N. Ivankin A. V. Berdutina 《Applied Biochemistry and Microbiology》2000,36(5):452-459
Properties of protein hydrolysates and possible uses of these substances in research and various branches of industry are
considered. The main problem discussed in this paper is the relationship between the degree of protein conversion and characteristics
(structural-functional and physicochemical) of hydrolysates. 相似文献
2.
Immobilization of aminoacylase from Streptoverticillium olivoreticuli by incorporation into acrylamide gel has been investigated. The data showed that the process should be carried out at constant pH. Thermal inactivation of the immobilized enzyme under the reaction conditions was studied. The kinetics of enzymatic stereospecific deacylation of N-acetyl-DL-phenylalanine was analyzed. The results of this study may be used in the synthesis of amino acid enantiomers. 相似文献
3.
Microbiological and biotechnological characteristics of intensification of aerobic processing of organic waste have been reviewed, with a view for revealing two types of correlations: (1) between the quality of the composts obtained and the microorganisms involved in composting and (2) between physicochemical parameters and consumer properties of the composts. 相似文献
4.
The use of peptide hydrolase (EC 3.4.13.1) fromXanthomonas rubrilineans for synthesis of the antibiotic cephalexin from 7-aminodesacetoxycephalosporanic acid was studied. The optimum conditions
for production of cephalexin were determined, and the yield exceeded 80%. A method for monitoring the synthesis of this antibiotic
by means of a conventional amino acid analyzer is proposed. 相似文献
5.
A. D. Neklyudov A. N. Ivankin A. V. Berdutina 《Applied Biochemistry and Microbiology》2000,36(4):317-324
Recent achievements in the technology of producing protein hydrolysates are reviewed. Approaches to describing the mechanism
of hydrolysis and the possibility of purifying hydrolysates for their use in the food, medical, forage, and microbiological
industries are discussed. 相似文献
6.
A. V. Berdutina A. D. Neklyudov A. I. Ivankin B. S. Karpo S. I. Mitaleva 《Applied Biochemistry and Microbiology》2000,36(4):363-367
The proteolytic activity and thermal stability of the enzyme complex of a cell suspension from pig and bovine pancreas glands
was compared with those of pancreatin. The enzyme complex displayed the highest thermal stability and activity at 50°C. The
kinetic constants, energies of activation and inactivation of the enzyme complex, and pH optimum (7.0 ± 0.1) at which this
complex had the maximum proteolytic activity were determined. Pancreatin had a pH optimum of 8.0 ±0.1. 相似文献
7.
German A. B. Neklyudov A. D. Ivankin A. N. Berdutina A. V. 《Applied Biochemistry and Microbiology》2002,38(6):517-520
Hydrolysis of lipids from beef fat by pancreatic lipase was studied. The maximum release of free fatty acids was shown to occur at 40°C for the first 3 h of the experiment. After this, transetherification was predominant. The main kinetic parameters were the following: maximum hydrolysis rate, V = 1.25 ± 0.1 mg fat/ml min; Michaelis constant, K
M
H = 100 ± 12 mg fat/ml; constant of substrate inhibition, K
S = 10.0 ± 0.8 mg fat/ml; equilibrium constant, K
P = 277 ± 170 mg fat/ml; and activation energy of beef fat hydrolysis by pancreatic lipase, E
a = 19.1 ± 1.1 kJ/mole. The kinetic method used could be applied to development of the method for biotransformation of poorly assimilated fats into more valuable products. 相似文献
8.
Aerobic processing of organic waste into composts 总被引:4,自引:0,他引:4
A. D. Neklyudov G. N. Fedotov A. N. Ivankin 《Applied Biochemistry and Microbiology》2006,42(4):341-353
The current achievements in the field of aerobic waste processing into composts are reviewed, with an emphasis on amelioration of the environment and targeted use for intensification of agriculture. The methods for obtaining microbiological transformation products of household, agricultural, and industrial waste and their characteristics are analyzed. 相似文献
9.
Neklyudov A. D. Berdutina A. V. Ivankin A. N. Mitaleva S. I. Evstafeva E. A. 《Applied Biochemistry and Microbiology》2003,39(4):426-430
Collagen fractions have been isolated by water–salt extraction from raw materials of animal origin (various tendon types or subcutaneous tissues of cattle or porcine skin). Collagen fractions with maximum capacity for water and fat retention were isolated with high efficiency by water–salt solutions containing 1–10% sodium chloride at temperatures below 50°C. The values of the effective constant of extraction rate (min–1) at pH 6.5, 9.0, and 12.0 were equal to (2.7 ± 0.1) × 10–3, (6.2 ± 0.5) × 10–3, and (15.4 ± 0.7) × 10–3, respectively. The optimum conditions found made it possible to isolate from collagen those proteinaceous fractions that are of practical use in food industry. 相似文献
10.