排序方式: 共有2条查询结果,搜索用时 62 毫秒
1
1.
Juciano Gasparotto Matheus Augusto de Bittencourt Pasquali Nauana Somensi Laura Milán Vasques José Claudio Fonseca Moreira Reinaldo Nobrega de Almeida Jose Maria Barbosa-Filho Maria de Fátima Vanderlei de Souza Stanley Juan Chavez Gutierrez Lucindo José Quintans Júnior Daniel Pens Gelain 《Cell biology and toxicology》2013,29(3):175-187
Immunomodulatory actions exerted by some classes of tryptamines, such as benzoyltryptamine analogues, suggest these molecules as promising candidates to develop new therapies to treat conditions associated to acute and chronic pain and inflammation. N-salicyloyltryptamine (STP) was observed to act as an anticonvulsive agent and exert antinociceptive effects in mouse. In the present work, we performed a screening of cytotoxic, cytoprotective, immunomodulatory, and redox properties of STP in RAW 264.7 macrophages challenged with hydrogen peroxide and LPS. Our results show that STP presents no cytotoxicity in the range of 0.001 to 1 μg/mL, but doses of 50 and 100 μg/mL caused loss of cell viability (IC50?=?22.75 μg/mL). Similarly, STP at 0.001 to 1 μg/mL did not cause oxidative stress to RAW 264.7 cells, although it did not prevent cell death induced by H2O2 0.5 mM. At 1 μg/mL, STP reversed some redox and inflammatory parameters induced by LPS. These include thiol (sulfhydryl) oxidation, superoxide dismutase activation, and morphological changes associated to macrophage activation. Besides, STP significantly inhibited LPS-induced TNF-α and IL-1β release, as well as CD40 and TNF-α protein upregulation. Signaling events induced by LPS, such as phosphorylation of ERK 1/2 and IκBα and p65 nuclear translocation (NF-kB activation) were also inhibited by STP. These data indicate that STP is able to modulate inflammatory parameters at doses that do not interfere in cell viability. 相似文献
2.
Morrone Maurilio da Silva Somensi Nauana Franz Lucas Ramos Vitor de Miranda Gasparotto Juciano da Rosa Helen Taís Sartori Marcelo Figueiró Fabrício Gelain Daniel Pens Zanotto-Filho Alfeu Moreira José Cláudio Fonseca 《Molecular and cellular biochemistry》2019,453(1-2):11-32
Molecular and Cellular Biochemistry - Inadequate migration and invasion of the trophoblast cells during embryo implantation is one of the reasons for pregnancy-related complications such as... 相似文献
1