Ataxia-telangiectasia: Linkage analysis in highly inbred Arab and Druze families and differentiation from an ataxia-microcephaly-cataract syndrome

Summary Ataxia-telangiectasia (A-T) is a progressive autosomal recessive disease featuring neurodegeneration, immunodeficiency, chromosomal instability, radiation sensitivity and a highly increased proneness to cancer. A-T is ethnically widespread and genetically heterogeneous, as indicated by the existence of four complementation groups in this disease. Several A-T-like genetic diseases share various clinical and cellular characteristics with A-T. By using linkage analysis to study North American and Turkish A-O families, the ATA (A-T, complementation group A) gene has been mapped to chromosome 11q23. A number of Israeli Arab A-T patients coming from large, highly inbred families were assigned to group A In one of these families, an additional autosomal recessive disease was identified, characterized by ataxia, hypotonia, microcephaly and bilateral congenital cataracts. In two patients with this syndrome, normal levels of serum immunoglobulins and alpha-fetoprotein, chromosomal stability in peripheral blood lymphocytes and skin fibroblasts, and normal cellular response to treatments with X-rays and the radiomimetic drug neocarzinostatin indicated that this disease does not share, with A-T, any additional features other than ataxia. These tests also showed that another patient in this family, who is also mentally retarded, is affected with both disorders. This conclusion was further supported by linkage analysis with 11q23 markers. Lod scores between A-O and these markers, cumulated over three large Arab families, were significant and confirmed the localization of the ATA gene to aq23. However, another Druze family unassigned to a specific complementation group, showed several recombinants between A-T and the same markers, leaving the localization of the A-T gene in this family open.     

Location of RNase activity in nuclear residual structures

We report here the partial isolation of an RNase activity which copurifies with the nuclear residual structure prepared from both rat liver and Herpes-infected BHK cells. After the successive treatment of purified nuclei with DNase, low salt and high salt, the RNase activity is found both in the high salt soluble supernatant fraction and in the residual nuclear structure. Triton X-100 treatment of this structure solubilizes the RNase activity. From this we conclude that some of the RNase activity associated with the nuclear residual structure may be located in either the phospholipidic or protein moieties that were extracted with Triton X-100. This RNase cuts rRNA non-randomly into characteristic degradation products. Its molecular weight, on a glycerol gradient, was determined to be 25,000.     

Wet meadows of the alliance <Emphasis Type="Italic">Molinion</Emphasis> and their environmental gradients in Slovenia

The main objective of this study was to examine the relationships between wet meadow plant communities of Molinon alliance and their environmental conditions in Slovenia. The ecology of these communities was analysed in detail. The study provides the data on the vegetation and environmental parameters, the significance of parameters for the plant species composition, most important environmental gradients and differences between plant communities. In all plots the vegetation was recorded and soil parameters were analysed (pH, plant-available P and K, N^t, organic C, C/N ratio, exchangeable Ca²⁺, Mg²⁺, K⁺, Na⁺, H⁺, electrical conductivity, base saturation). Other conditions were also considered (e.g., mean annual temperature and precipitation, humidity index, mean Ellenberg moisture and nutrient value) to test possible correlations as well. Vegetation was classified by means of multivariate cluster analysis, while vegetation-site relationships were examined with direct gradient analysis (CCA). Six associations from the Molinon alliance (Selino-Molinietum, Plantagini altissimae-Molinietum, Carici davallianae-Molinietum, Gentiano-Molinietum litoralis, Junco conglomerati-Betonicetum and Sanguisorbo-Festucetum commutatae) were identified and analysed. Soil reaction was identified as most significant environmental parameter explaining the variation of the studied vegetation. There are several statistically significant differences in site conditions between the communities (pH, moisture, nutrient status, Ca²⁺). The studied associations represent clearly defined ecological units.     

High-integrity human intervention in ecosystems: Tracking self-organization modes

Humans play major roles in shaping and transforming the ecology of Earth. Unlike natural drivers of ecosystem change, which are erratic and unpredictable, human intervention in ecosystems generally involves planning and management, but often results in detrimental outcomes. Using model studies and aerial-image analysis, we argue that the design of a successful human intervention form calls for the identification of the self-organization modes that drive ecosystem change, and for studying their dynamics. We demonstrate this approach with two examples: grazing management in drought-prone ecosystems, and rehabilitation of degraded vegetation by water harvesting. We show that grazing can increase the resilience to droughts, rather than imposing an additional stress, if managed in a spatially non-uniform manner, and that fragmental restoration along contour bunds is more resilient than the common practice of continuous restoration in vegetation stripes. We conclude by discussing the need for additional studies of self-organization modes and their dynamics.     

Scaling‐up biodiversity‐ecosystem functioning research

A rich body of knowledge links biodiversity to ecosystem functioning (BEF), but it is primarily focused on small scales. We review the current theory and identify six expectations for scale dependence in the BEF relationship: (1) a nonlinear change in the slope of the BEF relationship with spatial scale; (2) a scale‐dependent relationship between ecosystem stability and spatial extent; (3) coexistence within and among sites will result in a positive BEF relationship at larger scales; (4) temporal autocorrelation in environmental variability affects species turnover and thus the change in BEF slope with scale; (5) connectivity in metacommunities generates nonlinear BEF and stability relationships by affecting population  synchrony at local and regional scales; (6) spatial scaling in food web structure and diversity will generate scale dependence in ecosystem functioning. We suggest directions for synthesis that combine approaches in metaecosystem and metacommunity ecology and integrate cross‐scale feedbacks. Tests of this theory may combine remote sensing with a generation of networked experiments that assess effects at multiple scales. We also show how anthropogenic land cover change may alter the scaling of the BEF relationship. New research on the role of scale in BEF will guide policy linking the goals of managing biodiversity and ecosystems.     

The effect of exercise on plasma cortisol and blood sugar levels in the rainbow trout, Salmo gairdnerii Richardson

Preliminary experiments were performed to determine the diurnal variation in cortisol, using trout which had been cannulated three days previously. These results indicated that cortisol levels were reasonably stable between 10.00 and 18.00 hours, thus permitting experimentation during this period without diurnal fluctuations masking the cortisol response. Uncannulated fish were exercised in a flume for 2 h at 1, 2.6 and 5 bl s^-1 and plasma samples taken from groups of five animals at 15, 30, 60 and 120 min after the start of exercise and at 1½, 12 and 24 h after the exercise ceased. The cortisol levels in all cases were elevated after 15 min, but the magnitude of the elevation increased with swimming speed. At 1 bl s^-1 the cortisol levels increased from 76.4 (± 20.4) to 129.2 (± 20.4) ng ml^-1 [mean (± s.d. )]. At 2.6 bl s^-1 the increase was from 72.4 (± 17.1) to 254.4 (± 34.4) ng ml^-1 and at 5 bl s^-1 the increase was from 69.5 (± 27.5) to 326.4 (± 39.0) ng ml^-1. The cortisol levels were stable over the exercise period and all groups recovered to baseline levels after 24 h, though the sample taken 12 h after the termination of exercise was elevated due to regular nocturnal increases in cortisol levels. There were no dramatic changes in blood sugar levels during and after exercise at 1 and 3.2 bl s^-1.