排序方式: 共有11条查询结果,搜索用时 15 毫秒
1.
Olfa Ayed-Slama Imen Bouhaouel Zoubeir Chamekh Youssef Trifa Ali Sahli Nadhira Ben Aissa Hajer Slim-Amara 《Journal of Genetic Engineering and Biotechnology》2018,16(1):161-167
Agriculture has new challenges against the climate change: the preservation of genetic resources and the rapid creation of new varieties better adapted to abiotic stress, specially salinity. In this context, the agronomic performance of 25 durum wheat (Triticum turgidum subsp. durum Desf.) genotypes (nineteen landraces and six improved varieties), cultivated in two semi-arid regions in the center area of Tunisia, were assessed. These sites (Echbika, 2.2?g?l?1; Barrouta, 4.2?g?l?1) differ by their degree of salinity of the water irrigation. The results showed that most of the agronomic traits (e.g. spike per meter square, thousand kernels weight and grain yield) were reduced by salinity. Durum wheat landraces, Mahmoudi and Hmira, and improved varieties, Maali and Om Rabia showed the widest adaptability to different quality of irrigation water. Genotypes including Jneh Kotifa and Arbi were estimated as stable genotypes under adverse conditions. Thereafter, salt-tolerant (Hmira and Jneh Khotifa) and the most cultivated high-yielding (Karim, Razzak and Khiar) genotypes were tested for their gynogenetic ability to obtain haploids and doubled haploid lines. Genotypes with good induction capacity had not necessarily a good capacity of regeneration of haploid plantlets. In our conditions, Hmira and Khiar exhibited the best gynogenetic ability (3.1% and 2.9% of haploid plantlets, respectively). 相似文献
2.
Kamila Tabet-Aoul Anne Oustry-Vaiman Daniel Vaiman Nadhira Sa?di-Mehtar Edmond-Paul Cribiu Frédéric Lantier 《遗传、选种与进化》2000,32(4):441-457
In order to simultaneously integrate linkage and syntenic groups to the ovine chromosomal map, a sheep bacterial artificial chromosome (BAC) library was screened with previously assigned microsatellites using a sheep-hamster hybrid panel and genetic linkage. Thirty-three BACs were obtained, fluorescently labelled and hybridised on sheep-goat hybrid metaphases (2n = 57). This study allowed us, (i), to anchor all linkage groups on sheep chromosomes, (ii), to give information on the probable position of the centromere on the linkage map for the centromeric chromosomes, (iii), to contradict the previous orientation of the ovine × linkage group by the mapping of BMS1008 on OARXq38. Concerning our somatic cell hybrid panel, this study resulted in the assignment of all the previously unassigned groups to ovine chromosomes and a complete characterisation of the hybrid panel. In addition, since hybridisations were performed on a sheep-goat hybrid, new marker/anchoring points were added to the caprine cytogenetic map. 相似文献
3.
Kamila Tabet-Aoul Laurent Schibler Daniel Vaiman Anne Oustry-Vaiman Isabelle Lantier Nadhira Saidi-Mehtar Edmond-Paul Cribiu Frédéric Lantier 《Mammalian genome》2000,11(1):37-40
The regional characterization of a previously obtained hamster–sheep hybrid panel is reported. Using data available from
ruminant maps (sheep, cattle, and goat), we have selected a set of 300 markers and have analyzed them by PCR in this hybrid
panel. Results obtained for 204 markers show the presence of all sheep chromosomes (including gonosomes) in entire or fragmented
form. Analysis of syntenies has given 130 types of answer defining segments of variable sizes. This study has led to the regional
characterization of this panel and provides comparative data on a set of bovine and caprine markers. With the level of characterization
now achieved for this hybrid panel, the regional assignment of new genes or markers to sheep chromosomes can be rapidly obtained.
Finally, this panel will help to collect new data for comparative mapping of domestic animals and to highlight the conservation
of syntenic groups between closely related species, that is, sheep, cattle, and goat.
Received: 14 May 1999 / Accepted: 23 August 1999 相似文献
4.
M S Gross A Sefiani M F de Tand Y F Lau N Saidi-Mehtar M C Hors-Cayla J Frezal N Van Cong 《Annales de génétique》1989,32(2):73-77
An anonymous DNA probe PAS45 was isolated. This probe detects an RFLP with two alleles 1 and 2 at the same locus, with the different restriction enzymes (Bg1II, EcoRI, HindIII, PstI, MspI, XbaI). The observed polymorphism is explained by a chromosome rearrangement involving these enzyme cleavage sites. The frequency of alleles 1 and 2 was 0.875 and 0.125, respectively, in a sample of 48 unrelated individuals in France. Codominant inheritance of alleles 1 and 2 was demonstrated in 13 families with 30 offspring. The PAS45 probe was localized on chromosome 13 by somatic cell hybrid analysis and on 13q31 by in situ hybridization. The rearrangement on 13q31 is present in one out of four healthy individuals in France. 相似文献
5.
Laurence Flori Katayoun Moazami‐Goudarzi Vronique Alary Abdelillah Araba Ismaïl Boujenane Nadjet Boushaba Franois Casabianca Sara Casu Roberta Ciampolini Armelle Coeur D'Acier Corinne Coquelle Juan‐Vicente Delgado Ahmed El‐Beltagi Georgia Hadjipavlou Emmanuelle Jousselin Vincenzo Landi Anne Lauvie Philippe Lecomte Christina Ligda Caroline Marinthe Amparo Martinez Salvatore Mastrangelo Dalal Menni Charles‐Henri Moulin Mona‐Abdelzaher Osman Olivier Pineau Baldassare Portolano Clementina Rodellar Nadhira Saïdi‐Mehtar Tiziana Sechi Guilhem Sempr Sophie Thvenon Dimitrios Tsiokos Denis Laloë Mathieu Gautier 《Molecular ecology》2019,28(5):1009-1029
Domestic species such as cattle (Bos taurus taurus and B. t. indicus) represent attractive biological models to characterize the genetic basis of short‐term evolutionary response to climate pressure induced by their post‐domestication history. Here, using newly generated dense SNP genotyping data, we assessed the structuring of genetic diversity of 21 autochtonous cattle breeds from the whole Mediterranean basin and performed genome‐wide association analyses with covariables discriminating the different Mediterranean climate subtypes. This provided insights into both the demographic and adaptive histories of Mediterranean cattle. In particular, a detailed functional annotation of genes surrounding variants associated with climate variations highlighted several biological functions involved in Mediterranean climate adaptation such as thermotolerance, UV protection, pathogen resistance or metabolism with strong candidate genes identified (e.g., NDUFB3, FBN1, METTL3, LEF1, ANTXR2 and TCF7). Accordingly, our results suggest that main selective pressures affecting cattle in Mediterranean area may have been related to variation in heat and UV exposure, in food resources availability and in exposure to pathogens, such as anthrax bacteria (Bacillus anthracis). Furthermore, the observed contribution of the three main bovine ancestries (indicine, European and African taurine) in these different populations suggested that adaptation to local climate conditions may have either relied on standing genomic variation of taurine origin, or adaptive introgression from indicine origin, depending on the local breed origins. Taken together, our results highlight the genetic uniqueness of local Mediterranean cattle breeds and strongly support conservation of these populations. 相似文献
6.
Nejla Hechmi Nadhira Ben Aissa Hassen Abdenaceur Naceur Jedidi 《International journal of phytoremediation》2014,16(12):1241-1256
Bioremediation of soil polluted by pentachlorophenol (PCP) is of great importance due to the persistence and carcinogenic properties of PCP. Phytoremediation has long been recognized as a promising approach for removal of PCP from soil. The present study was conducted to investigate the capability of four plant species; white clover, ryegrass, alfalfa, and rapeseed grown alone and in combination to remediate pentachlorophenol contaminated soil. After 60 days cultivation, white clover, raygrass, alfalfa, and rapeseed all significantly enhanced the degradation of PCP in soils. Alfalfa showed highest efficiency for the removal of PCP in single cropping flowed by rapeseed and ryegrass. Mixed cropping significantly enhanced the remediation efficiencies as compared to single cropping; about 89.84% of PCP was removed by mixed cropping of rapeseed and alfalfa, and 72.01% of PCP by mixed cropping of rape and white clover. Mixed cropping of rapeseed with alfalfa was however far better for the remediation of soil PCP than single cropping. An evaluation of soil biological activities as a monitoring mechanism for the bioremediation process of a PCP-contaminated soil was made using measurements of microbial counts and dehydrogenase activity. 相似文献
7.
Samir Bachir Souheil Gaouar Anne Da Silva Elena Ciani Samia Kdidi Miloud Aouissat Laziz Dhimi Mohamed Lafri Abderrahman Maftah Nadhira Mehtar 《PloS one》2015,10(4)
Due to its geo-climatic conditions, Algeria represents a biodiversity hotspot, with sheep breeds well adapted to a patchwork of extremely heterogeneous harsh habitats. The importance of this peculiar genetic reservoir increases as climate change drives the demand for new adaptations. However, the expansion of a single breed (Ouled-Djellal) which occurred in the last decades has generated a critical situation for the other breeds; some of them are being subjected to uncontrolled cross-breeding with the favored breed and/or to marginalization (effective size contraction). This study investigated genetic diversity within and among six of the nine Algerian breeds, by use of 30 microsatellite markers. Our results showed that, in spite of the census contraction experienced by most of the considered breeds, genetic diversity is still substantial (average gene diversity ranging 0.68 to 0.76) and inbreeding was not identified as a problem. However, two breeds (Rembi and Taâdmit) appeared to have lost most of their genetic originality because of intensive cross-breeding with Ouled-Djellal. Based on the above evidence, we suggest Hamra, Sidaoun, and D’man as breeds deserving the highest priority for conservation in Algeria. 相似文献
8.
Nejla Hechmi Nadhira Ben Aissa Hassen Abdenaceur Naceur Jedidi 《International journal of phytoremediation》2015,17(2):109-116
Despite many studies on phytoremediation of soils contaminated with either heavy metals or organics, little information is available on the effectiveness of phytoremediation of co-occurring metal and organic pollutants especially by using wetland species. Phragmites australis is a common wetland plant and its potential for phytoremediation of cadmium pentachlorophenol (Cd-PCP) co-contaminated soil was investigated. A greenhouse study was executed to elucidate the effects of Cd (0, 10, and 20 mg kg?1) without or with PCP (0, 50, and 250 mg kg?1) on the growth of the wetland plant P. australis and its uptake, accumulation and removal of pollutant from soils. After 75 days, plant biomass was significantly influenced by interaction of Cd and PCP and the effect of Cd on plant growth being stronger than that of PCP. Coexistence of PCP at low level lessened Cd toxicity to plants, resulting in improved plant growth and increased Cd accumulation in plant tissues. The dissipation of PCP in soils was significantly influenced by interactions of Cd, PCP and plant presence or absence. As an evaluation of soil biological activities after remediation soil enzyme was measured. 相似文献
9.
10.
Twenty-four hamster-sheep hybrid cell lines representing eleven ovine synteny groups were used to make syntenic assignments for seven loci ALDOB (aldolase B, fructose biphosphate); AMH (anti-Müllerian hormone); CYP19 [cytochrome P450 aromatase, subfamily XIX (aromatization of androgens)]; WT (Wilms' tumour gene); SOX2 (SRY-related HMG-box gene 2); FSHB (follicle-stimulating hormone, beta polypeptide); and SRY (sex region of Y chromosome). These loci were assigned to synteny groups U11(chr2) ( ALDOB ); U19 ( AMH ); U3(chr7) ( CYP19 ); and to chromosomes 15 ( WT ) and 1 ( SOX2 ). SRY defines the hybrids containing the Y chromosome. 相似文献