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排序方式: 共有56条查询结果,搜索用时 15 毫秒
1.
SATORU KOBAYASHI HIDEKO MIZUNO MASUKICHI OKADA 《Development, growth & differentiation》1988,30(3):251-260
We describe the accumulation and distribution of poly (A)+ RNA during oogenesis and early embryogenesis as revealed by in situ hybridization with a radio-labeled poly (U) probe. The amount of poly (A)+ RNA in nurse cell cytoplasm continuously increased untill mid-vitellogenic stage (st. 10), then decreased with the rapid increase of poly (A)+ RNA in the oocyte (st. 11). The localization of poly (A)+ RNA at stage 10 was in the anterior region of the oocyte, where it is connected by cytoplasmic bridge to the nurse cells. These observations indicate that most of the poly (A)+ RNA synthesized in the nurse cells is transferred to the oocyte through the cytoplasmic bridges at stage 10–11. During the remainder of oogenesis (st. 11–14) and during preblastodermal embryogenesis, poly (A)+ RNA was evenly distributed over the cytoplasm of oocytes and embryos. At blastoderm stage, poly(A)+ RNA became concentrated in the peripheral region of embryos. Though the somatic nuclei of the blastoderm contained a detectable amount of poly (A)+ RNA, the pole cell nuclei did not. The cytoplasmic RNA visualised by acridine orange staining and the poly (A)+ RNA detected by hybridization with [3 H]poly (U) exhibited identical distributions during oogenesis and early embryogenesis. These observations provide a basis to assess the unique distributions of specific RNA sequences involved in early development. 相似文献
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Abstract We tested the hypothesis that electrogenic ion pumps, working at the parenchyma symplast/xylem interface of pea hypocotyls, provide the driving force for K+ uptake from the xylem. Solutions of known composition were perfused through a hypocotyl segment. The K+ activity of the solution flowing out of the xylem (K+out) increased (i.e. K+ uptake decreased) when aerobic respiration was inhibited by lack of O2, and this was preceded by a decrease in Vpx (electrical potential difference between parenchyma symplast and xylem). Perfusion with auxin (1AA) and fusicoccin (FC) stimulated the electrogenic activity of the ‘xylem pumps’ (111 and 205% respectively) and stimulated uptake of K + from the xylem (with 71% and 29% respectively). The close correlation between xylem pump activity and K+ uptake corroborated the aforementioned hypothesis. Interestingly, inhibition of pump activity by anoxia was incomplete in the presence of FC. It is thought that FC increases the affinity of the ATP-requiring xylem pump for ATP, thus ensuring that ATP production during fermentation is sufficient to fuel the pump in the absence of O2. 相似文献
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HISASHI OKAMOTO AKIKO MIZUNO KIYOSHI KATOU YOSHINA ONO YASUKO MATSUMURA HIROSHI KOJIMA 《Plant, cell & environment》1984,7(2):139-147
Abstract A new experimental system was devised for the simultaneous measurement of elongation rate and the activity of the spatially separate electrogenic ion pumps of a hypocotyl segment excised from a seedling of Vigna unguiculata L. Walp. under enforced intra-organ perfusion by artificial solutions. The pathway of the perfusion medium was apoplastic space, including xylem vessels as main routes. The elongation rate of the segment was highly dependent on the perfusion pressure applied. It was possible to increase the growth rate under pressurized perfusion by 10-30 times as much as that without perfusion. Elongation rate was also dependent on respiration under perfusion, being retarded reversibly by anoxia a few minutes after the activities of the electrogenic ion pumps were stopped. Perfusion pressure had a little influence on the membrane potential (Vpx) below a breakdown level (c. 130 kPa). Perfusion of mannitol or sorbitol solution of appropriate concentration reduced the elongation rate reversibly. 相似文献
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Several indices of reproductive skew, which quantify the degree of unequal partitioning of reproductive output among members
in an animal society, have been proposed. Here we point out the drawbacks of these indices. The most serious problem is the
dependence of the indices on mean reproductive success: skew values tend to be larger, as average numbers of offspring decrease,
due to random sampling error in numbers of offspring. Thus it is difficult to compare societies with different average lifetime
reproductive success using these indices, even though we have presented methods to calculate the expected reproductive skew
caused by random sampling error, especially when average numbers of offspring are small, as is often the case with cooperatively
breeding vertebrates. As an alternative, we propose using the spatial dispersion indices of population ecology (Morisita's
index or its standardized version) for the measurement of reproductive skew. These indices are almost independent of average
fecundity and have their own method of testing for random variation in offspring numbers.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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TOSHIHIKO MIZUNO SYUITI MORI 《Biological journal of the Linnean Society. Linnean Society of London》1970,2(2):77-118
The preliminary research in some tropical inland waters of Asia is described and suggestions made concerning Phase II of this project undertaken by the International Biological Programme/ Section PF (Freshwater Productivity). Environmental factors were measured in some of the main lakes and rivers and samples taken of the plant and animal life. The stomach contents of over 80 species of fish were examined and the remains of plants and animals present compared with the numbers present in the environment as obtained by the usual hydrobiological netting techniques. Some of the important problems associated with the biological productivity of these waters is discussed in the light of the results obtained from this preliminary survey. 相似文献