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Muzna Zahur Asma Maqbool Muhammad Irfan Muhammad Younas Khan Barozai Bushra Rashid Shiekh Riazuddin Tayyab Husnain 《Molecular Biology》2009,43(4):578-585
The 949 bp promoter fragment upstream from the translation initiation site of the GUSP gene encoding a universal stress protein was isolated from the genomic DNA of Gossypium arboreum. Some putative cis-acting elements involved in stress responses including E-box, ABRE, DPBF-box, and MYB-core elements were found in the promoter
region. In an Agrobacterium-mediated transient expression assay, strong activation of the GUSP full promoter region occurred in tobacco leaves following dehydration, abscisic acid, salt, heavy metal, gibberellic acid
and dark treatments. Deletion analysis of the promoter revealed that the dehydration, abscisic acid and salt responses were
affected by the deletion between −208 and −949 bp and showed 2–4-fold induction. However, in response to dark, gibberellic
acid and heavy metals the induction was only 2-fold. These findings further our understanding of the regulation of GUSP expression. This is an important study as no report of this universal stress protein promoter is available in literature. 相似文献
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Asma Maqbool Waseem Abbas Abdul Qayyum Rao Muhammad Irfan Muzna Zahur Allah Bakhsh Shiekh Riazuddin Tayyab Husnain 《Biotechnology progress》2010,26(1):21-25
Heat‐shock proteins (HSP) are molecular chaperones for protein molecules. These proteins play an important role in protein–protein interactions such as, folding and assisting in the establishment of proper protein conformation and prevention of unwanted protein aggregation. A small HSP gene GHSP26 present in Gossypium arboreum responds to dehydration. In the present study, an attempt was made to overcome the problem of drought stress in cotton. A cDNA of GHSP26 was isolated from G. arboreum, cloned in plant expression vector, pCAMBIA‐1301 driven by the cauliflower mosaic virus 35S promoter and introduced into Gossypium hirsutum. The integration and expression studies of putative transgenic plants were performed through GUS assay; PCR from genomic DNA, and quantitative real‐time PCR analysis. Transgenic cotton plants showed an enhanced drought tolerance, suggesting that GHSP26 may play a role in plant responsiveness to drought. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010 相似文献
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Yuan Hou Yadi Zhou Muzna Hussain G. Thomas Budd Wai Hong Wilson Tang James Abraham Bo Xu Chirag Shah Rohit Moudgil Zoran Popovic Chris Watson Leslie Cho Mina Chung Mohamed Kanj Samir Kapadia Brian Griffin Lars Svensson Patrick Collier Feixiong Cheng 《PLoS medicine》2021,18(8)
BackgroundCardiovascular disease is a leading cause of death in general population and the second leading cause of mortality and morbidity in cancer survivors after recurrent malignancy in the United States. The growing awareness of cancer therapy–related cardiac dysfunction (CTRCD) has led to an emerging field of cardio-oncology; yet, there is limited knowledge on how to predict which patients will experience adverse cardiac outcomes. We aimed to perform unbiased cardiac risk stratification for cancer patients using our large-scale, institutional electronic medical records.Methods and findingsWe built a large longitudinal (up to 22 years’ follow-up from March 1997 to January 2019) cardio-oncology cohort having 4,632 cancer patients in Cleveland Clinic with 5 diagnosed cardiac outcomes: atrial fibrillation, coronary artery disease, heart failure, myocardial infarction, and stroke. The entire population includes 84% white Americans and 11% black Americans, and 59% females versus 41% males, with median age of 63 (interquartile range [IQR]: 54 to 71) years old.We utilized a topology-based K-means clustering approach for unbiased patient–patient network analyses of data from general demographics, echocardiogram (over 25,000), lab testing, and cardiac factors (cardiac). We performed hazard ratio (HR) and Kaplan–Meier analyses to identify clinically actionable variables. All confounding factors were adjusted by Cox regression models. We performed random-split and time-split training-test validation for our model.We identified 4 clinically relevant subgroups that are significantly correlated with incidence of cardiac outcomes and mortality. Among the 4 subgroups, subgroup I (n = 625) has the highest risk of de novo CTRCD (28%) with an HR of 3.05 (95% confidence interval (CI) 2.51 to 3.72). Patients in subgroup IV (n = 1,250) had the worst survival probability (HR 4.32, 95% CI 3.82 to 4.88). From longitudinal patient–patient network analyses, the patients in subgroup I had a higher percentage of de novo CTRCD and a worse mortality within 5 years after the initiation of cancer therapies compared to long-time exposure (6 to 20 years). Using clinical variable network analyses, we identified that serum levels of NT-proB-type Natriuretic Peptide (NT-proBNP) and Troponin T are significantly correlated with patient’s mortality (NT-proBNP > 900 pg/mL versus NT-proBNP = 0 to 125 pg/mL, HR = 2.95, 95% CI 2.28 to 3.82, p < 0.001; Troponin T > 0.05 μg/L versus Troponin T ≤ 0.01 μg/L, HR = 2.08, 95% CI 1.83 to 2.34, p < 0.001). Study limitations include lack of independent cardio-oncology cohorts from different healthcare systems to evaluate the generalizability of the models. Meanwhile, the confounding factors, such as multiple medication usages, may influence the findings.ConclusionsIn this study, we demonstrated that the patient–patient network clustering methodology is clinically intuitive, and it allows more rapid identification of cancer survivors that are at greater risk of cardiac dysfunction. We believed that this study holds great promise for identifying novel cardiac risk subgroups and clinically actionable variables for the development of precision cardio-oncology.Yuan Hou and co-workers investigate risk of cardiac dysfunction in cancer patients. 相似文献
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Zahur Zaman Marie-Jeanne Heynen Robrecht L. Verwilghen 《Biochimica et Biophysica Acta (BBA)/General Subjects》1980,632(4):553-561
Mechanism of transferrin iron uptake by rat reticulocytes was studied using 59Fe- and 125I-labelled rat transferrin. Whereas more than 80% of the reticulocyte-bound 59Fe was located in the cytoplasmic fraction, only 25–30% of 125I-labelled transferrin was found inside the cells. As shown by the presence of acetylcholine esterase, 10–15% of the cytoplasmic 125I-labelled transferrin might have been derived from the contamination of this fraction by the plasma membrane fragments. Electron microscopic autoradiography indicated 26% of the cell-bound 125I-labelled transferrin to be inside the reticulocytes. Both the electron microscopic and biochemical studies showed that the rat reticulocytes endocytosed their plasma membrane independently of transferrin. Sepharose-linked transferrin was found to be capable of delivering 59Fe to the reticulocytes. Our results suggest that penetration of the cell membrane by transferrin is not necessary for the delivery of iron and that, although it might make a contribution to the cellular iron uptake, internalization of transferrin reflects endocytotic activity of the reticulocyte cell membrane. 相似文献
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Amber Afroz Afsari Qureshi Muzna Zahur Umer Rashid Hamid Rashid 《Plant Molecular Biology Reporter》2012,30(1):204-213
An efficient transformation system for rice was established by co-cultivating calli, derived from 21-day-old scutellum, with
Agrobacterium tumefaciens cells (OD600 = 0.04), maintained on filter paper, moistened with 4 mL of a medium, and supplemented with 400 μM acetosyringone,
for a period of 2 days. Presence of the transgene was confirmed by polymerase chain reaction, and stable integration and copy
number of the transgene were determined by Southern blot analysis. Among seven plants analyzed, six possessed single T-DNA
integration events, while one plant was found to have two integrated copies of the T-DNA. A total of 45 T0 plants were grown in the greenhouse to obtain the T1 generation. T1 plants evaluated for presence of the transgene and for response to inoculation with the bacterial leaf blight pathogen, Xanthomonas oryzae pv. Oryzae, exhibited Mendelian segregation (3:1) for the transgene as well as enhanced resistance to bacterial blight 相似文献
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The dispersion of dye molecules and small cations injected from a point source in the cytoplasm of molluscan neurons has been measured photometrically and compared with dispersion in aqueous solution. The diffusion of phenol red and arsenazo III was at least a factor of five slower in the cytoplasm than in saline. Movement of both dyes was slowed by about the same factor in a given cell. The dispersion rate of arsenazo III was not significantly affected by preloading the cytoplasm to dye concentrations up to 0.5 mM. Calcium and barium dispersion was measured in neurons and saline droplets preloaded with arsenazo III, while phenol red absorbance changes were used to follow the dispersion of injected protons. Ba2+ and H+ moved very slowly in the cytoplasm compared to aqueous solution. Ca2+ movement in all probability underwent a similar retardation in the neurons but high-affinity buffering of the cytoplasm severely restricted the spread of detectable amounts of this ion away from the injection site. 相似文献
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