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排序方式: 共有59条查询结果,搜索用时 46 毫秒
1.
Pleshakova EV Muratova AIu Turkovskaia OV 《Prikladnaia biokhimiia i mikrobiologiia》2001,37(4):398-404
The Acinetobacter calcoaceticus strain TM-31 has been isolated from a microbial assemblage of a pilot plant purifying waste water polluted with mineral oil. This strain is capable of efficient degradation of components of mineral oil (alkanes, isoalkanes, and alkyl residues of the naphthene and arene fraction. The strain bears stably inherited plasmids of sizes 120, 9, and 8 kb, which can be transferred into plasmid-free cells of the parental strain and into bacteria of the genus Pseudomonas and ensure the degradation of hexadecane and mineral oil. 相似文献
2.
Pancreatic enzyme activity in early phases of acute experimental pancreatitis in rats 总被引:1,自引:0,他引:1
Saĭdalikhodzhaeva OZ Iuldashev NM Daniiarov AN Muratova UZ 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2002,88(4):526-529
Experiments were performed on laboratory rats with acute pancreatitis caused by local freezing the pancreas with chlorethyl. An active action of enzymes alpha-amilase, lipase, phospholipase A2, was revealed. During the first hours, an increase in action of all three enzymes, particularly that of phospholipase A2, was found. It was established that the lipid spectrum of pancreas had changed. It shows that cell membranes were destroyed. Experiments revealed an activating role of Ca2+ ions for all the enzymes and a correcting action of chlorpromasine. 相似文献
3.
Muratova AIu Turkovskaia OV Hubner T Kuschk P 《Prikladnaia biokhimiia i mikrobiologiia》2003,39(6):681-688
The efficacy of plants as means of decontaminating hydrocarbon-polluted soil has been studied. Ditch reed (Phragmites australis) and alfalfa (Medicago sativa) markedly intensified processes of pollutant destruction, the effect being particularly pronounced in the case of polycyclic aromatic hydrocarbons (PAHs). Comparative analysis of microflora in soils (including those devoid of plants and rhizosphere) demonstrated that, in addition to preventing the pollutant-induced decrease in the amount of heterotrophic microorganisms, the plants stimulated their development, significantly increasing the population of destructors. Effects of plants on major physiological groups of soil microorganisms under conditions of pollution were ambiguous. The rhizosphere consortium of alfalfa was less susceptible to effects of pollutants than that of reed. 相似文献
4.
E. V. Bazhina O. V. Kvitko E. N. Muratova 《Russian Journal of Developmental Biology》2007,38(4):246-252
Meiosis was studied in the Siberian fir (Abies sibirica Ledeb.) at the arboretum of the Sukachev Institute of Forest. Specific features of meiosis in planted trees have been described. Both general and specific types of irregularities have been identified. The range of irregularities under the arboretum conditions was much wider than in natural ecosystems. 相似文献
5.
Muratova A. Yu. Panchenko L. V. Dubrovskaya E. V. Lyubun’ E. V. Golubev S. N. Sungurtseva I. Yu. Zakharevich A. M. Biktasheva L. R. Galitskaya P. Yu. Turkovskaya O. V. 《Microbiology》2022,91(5):514-522
Microbiology - A biopreparation consisting of the cells of Azospirillum brasilense strain SR80 immobilized on wood biochar was characterized. Sorption of oil by biochar and its colonization by... 相似文献
6.
O. A. Zyryanova L. I. Milyutin E. N. Muratova V. A. Ryzhkova A. Ya. Larionova T. S. Sedel’nikova M. A. Korets I. A. Mikhailova 《Contemporary Problems of Ecology》2008,1(1):22-28
Being of global ecological significance, boreal forests of Siberia located at the high latitudes of Northern Eurasia serve as a unique source of genetic, species, and ecosystem diversity. Previously conducted research revealed a wide range of scientific problems regarding the intraspecific diversity of trees, estimation of the structure and current state of forest ecosystems, as well as their ordination and classification. The use of modern information technologies (databases, species diversity indices, GIS) can promote the knowledge on Siberian boreal forests to a higher level. 相似文献
7.
M U Tu?chibaev F A Muksimov N Akhmedova A V Shkinev U Z Mirkhodzhaev U Z Muratova K T Almatov M M Rakhimov B A Ashmukhamedov 《Biokhimii?a (Moscow, Russia)》1977,42(12):2160-2167
The molecular weight distribution of the components of giant hornet (Vespa orientalis) venom was studied, using gel-filtration on a column with Sephadex G-50. The effects of the venom and its constituent fractions on the permeability and stability of artificial bilayer phospholipid membranes, potassium ions release from the erythrocytes and mitochondrial oxidative phosphorylation parameters, as well as on the activity and stability of polyenzymic systems of the mitochondrial respiratroy chain, were studied. The data obtained suggest that the high molecular weight fractions contain phospholipases, whose activities are much higher than those of presently known venoms. Despite the fact that the hemolytic effect is typical for two low molecular weight fractions, no fractions possessing high activity of bee venom of the melitin type were found. 相似文献
8.
U Z Muratova V T Bornikov A Kh Abdukaiumova T S Saatov 《Biokhimii?a (Moscow, Russia)》1991,56(2):320-325
The activity of phospholipase A2 in blood platelets of healthy donors and IHD patients was examined. The enzyme activity was found to be increased 3-fold in platelets possessing a high level of functional activity (IHD) and by one order of magnitude in patients with myocardial infarction as compared with healthy donors. An enzyme preparation possessing a phospholipase activity was isolated from platelets by using salt extraction (KCl) and sonication. Purification of the enzyme by affinity chromatography resulted in two protein peaks both having a phospholipase A2 activity, the purification and molecular masses of these fractions being 768- and 2200-fold, and 13.5 and 15 kDa, respectively. It was supposed that these proteins are substrate-specific forms of phospholipase A2. 相似文献
9.
10.
E. V. Dubrovskaya N. N. Pozdnyakova V. S. Grinev A. Yu. Muratova S. N. Golubev A. D. Bondarenkova O. V. Turkovskaya 《Russian Journal of Plant Physiology》2016,63(3):338-348
A dominant form of cationic peroxidase (PO-2) was isolated from sorghum (Sorghum bicolor L. Moench) roots and purified to electrophoretically homogeneous state. The enzyme is a monomer with mol wt of 49.7 kD. The optimum pH and the main catalytic constants (KM, Vmax, kcat) were determined for oxidation of the main substrates including Н2О2, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS), 2,7-diaminofluorene, syringaldazine, 2,6-dimethoxyphenol, and o-dianisidine. The KM values increased in the sequence: H2O2 < 2,7-diaminofluorene < ABTS < o-dianisidine, whereas the maximum turnover number (93.9 s–1) was found for 2,7-diaminofluorene. Based on the analysis of molecular and catalytic properties of the enzyme, it was proven that PO-2 is a typical cationic plant peroxidase. Polycyclic aromatic hydrocarbons (phenanthrene, anthracene, fluorene), 2,2'-diphenic acid, and Ni ions had no significant influence on the activity of PO-2. The enzyme was inhibited by p-aminobenzoic acid, NaN3, 1-naphthol, 9,10-anthraquinone, and 9,10-phenanthrenequinone. In the presence of NaN3, 1-naphthol, and 9,10-phenanthrenequinone, a mixed competitive/noncompetitive type of inhibition was noted. The peroxidase PO-2 was found to oxidize synthetic anthraquinone dyes, phenanthrene, and some oxygenated derivatives of polycyclic aromatic hydrocarbons (9-phenanthrol; 1-naphthol; and 1-hydroxy-2-naphthoic, salicylic, and 2,2'-diphenic acids), which indirectly confirms the coupled plant–microbial metabolism of these compounds in the root zone of sorghum. The results indicate that 9,10-phenanthrenequinone and 2,2'-diphenic acid are the products of peroxidase-catalyzed oxidation of 9-phenanthrol. 相似文献