Carbohydrate-binding sequences of the lectin genes in leguminous plants from the Galegeae and Hedysareae Tribes

The carbohydrate-binding sequences (CBS) of the lectin genes from legume plants of the genera Astragalus Lam., Oxytropis DC., and Hedysarum L. were determined. Computer-assisted analysis of nucleotide and predicted amino acid sequences of the lectin gene fragments examined revealed a high homology level between their CBS. At the same time, the CBS of Astragalus and Oxytropis were considerably different from the lectin gene CBS in the earlier examined representatives of the tribe Galegeae, Caragana frutex and C. arborescens. This fact probably points to the differences in the carbohydrate-binding specificity of the proteins examined, which can eventually affect their functional activity.     

Genetic diversity and phylogeny of root nodule bacteria entering into symbiosis with bitter peavine <Emphasis Type="Italic">Lathyrus vernus</Emphasis> (L.) Bernh.

The genetic diversity and phylogeny of root nodule bacteria entering into symbiosis with bitter peavine Lathyrus vernus (L.) Bernh. (Fabaceae) growing in various regions of the Republic of Bashkortostan were studied. RAPD analysis revealed a high degree of polymorphism of the DNA of the isolated strains giving evidence of the heterogeneity of the microorganisms in question. The study of the phylogeny of microsymbionts based on comparative analysis of the nucleotide sequences of 16S rRNA genes showed that the bacteria isolated from the plant nodules of L. vernus growing on the territory of Ufa and Beloretsk raions belonged to the species Rhizobium leguminosarum, whereas the microsymbionts of L. vernus growing on the territory of Tatyshly raion belonged to the species Rhizobium tropici,@ except for several strains of Rhizobium leguminosarum     

Hybrid peony (<Emphasis Type="Italic">Paeonia hybrida</Emphasis> Pall.), a rare endangered plant of Bashkir Trans-Uralian region: Autocorrelation analysis of the spatial genotype distribution under different environmental conditions

Hybrid peony (Paeonia hybrida Pall.) is listed as endangered in Russian Federation (since 1988) and in several regions of Russia. The structure of the isolated population from Bashkir Trans-Uralian region, separated by 1500 km from the main geographic range of this species, has been studied using isoenzyme markers from 11 loci. The population is split between two geographical locations, both characterized by a relatively high genetic diversity (mean allele number A = 1.9±0.3, percentage of polymorphic loci P = 0.54, observed heterozigosity H _O = 0.223±0.068, expected heterozigosity H _E = 0.229±0.067) and a quite low intergroup genetic differentiation (fixation index F _ST = 0.008, Nei’s genetic distance D = 0.011). In a subpopulation located near a swampy lake in dense cereal grass and steppe shrubby vegetation, autocorrelation analysis has revealed spatial structuring of genetic variability. In the other location, a stipa-forb steppe on a flat hill slope, a uniform spatial structure of the genotypes has been observed. The results are discussed with respect to the ecological characteristics of the species and the conservation of the genetic pool of the population in situ and ex situ.     

The role of macrophages in the tissues regeneration stimulated by the biomaterials

Allogenic grafted tissues are subjected to biodegradation and replaced by the regenerate. To minimize the immune response and improve the rebuilding of tissues there was developed a technology to treat tissues with a cells elimination and dosed out extraction of proteoglycanes (Alloplant^®). With aim to clarify the role of macrophages in the tissues regeneration resulting implantation the biomaterials 112 rats were injected the allogenic and xenogenic (rabbits) pulverized biomaterials in the form of suspension. Injections were performed subcutaneously into the animals back by the base of the tail. The control group (14 rats) were injected a physiologic saline. Animals were killed by ether inhalation on day 2, 4, 7, 14, 30, 90 and 180 and tissue sections were studied by light and electron microscopy. The study showed the key role of the macrophages in resorption of the allogenic biomaterial and formation of the newly-formed tissue. Implantation of the biomaterial induced activity a great number of the mature macrophages, which completely lysed and resorbed the biomaterial particles. Expression TNF was significantly higher whereas expression TGF-1 was significantly lower. With xenogenic biomaterial implantation there were less macrophages, their activity was restricted. Macrophages containing large vacuoles with an active endo- and exocytosis were revealed in the allogenic biomaterial implantation and were named matrix-forming macrophages. We may suppose that these macrophages synthesize (or re-synthesize) proteoglycan component of the newly-formed collagen fibers. There was put forward a hypothesis about the two component mechanism of the collagen fibers formation.     

Genetic Diversity and Phylogeny of Root Nodule Bacteria Isolated from Nodules of Plants of the Lupinaster Genus Inhabiting the Southern Urals

Russian Journal of Genetics - The genetic diversity and phylogeny of root nodule bacteria, microsymbionts of plants of the genus Lupinaster Adans. (L. albus Link and L. pentaphyllus Moench), were...