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Djaber Tazdaït Nadia Abdi Hakim Lounici Hocine Grib Nabil Mameri André Pauss 《Bioremediation Journal》2013,17(4):294-304
Acclimated activated sludge was examined for its ability to degrade malathion with and without the presence of glucose as a potential cometabolite substrate. In this study, a packed-bed reactor (PBR) using three kinds of biofilm carriers was employed for efficient degradation of malathion. The results obtained indicate that microorganisms tested were able to degrade malathion. The observed degradation rate of the pesticide in the presence of glucose was the same as without glucose. The activated sludge was found to be able to use malathion as the sole phosphorus source. In contrast, the degradation ability of the activated sludge was lost when the pesticide was used as the sole source of sulfur. The degradation capacity of the PBR was higher than the performance obtained with the batch reactor. The reactor packed with crushed olive kernels exhibited the best performance, allowing a total removal of malathion (10 mg/dm3) within 12 h. 相似文献
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Gana ML Kebbouche-Gana S Touzi A Zorgani MA Pauss A Lounici H Mameri N 《Journal of industrial microbiology & biotechnology》2011,38(3):391-404
The present study enlightens the role of the antagonistic potential of nonpathogenic strain B21 against sulfate-reducing bacteria
(SRB) consortium. The inhibitor effects of strain B21 were compared with those of the chemical biocide tetrakishydroxymethylphosphonium
sulfate (THPS), generally used in the petroleum industry. The biological inhibitor exhibited much better and effective performance.
Growth of SRB in coculture with bacteria strain B21 antagonist exhibited decline in SRB growth, reduction in production of
sulfides, with consumption of sulfate. The observed effect seems more important in comparison with the effect caused by the
tested biocide (THPS). Strain B21, a dominant facultative aerobic species, has salt growth requirement always above 5% (w/v)
salts with optimal concentration of 10–15%. Phylogenetic analysis based on partial 16S rRNA gene sequences showed that strain B21
is a member of the genus Bacillus, being most closely related to Bacillus qingdaonensis DQ115802 (94.0% sequence similarity), Bacillus aidingensis DQ504377 (94.0%), and Bacillus salarius AY667494 (92.2%). Comparative analysis of partial 16S rRNA gene sequence data plus physiological, biochemical, and phenotypic
features of the novel isolate and related species of Bacillus indicated that strain B21 may represent a novel species within the genus Bacillus, named Bacillus sp. (EMBL, FR671419). The results of this study indicate the application potential of Bacillus strain B21 as a biocontrol agent to fight corrosion in the oil industry. 相似文献
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Abdi N Hamdache F Belhocine D Grib H Lounici H Piron DL Mameri N 《Biochemical Engineering Journal》2000,6(3):177-183
This paper describes the enzymatic hydrolysis of solid residue of olive mill (OMRS) in a batch reactor with the Trichoderma reesei enzyme. Before enzymatic saccharification, crude lignocellulosic material is submitted to alkaline pre-treatment with NaOH. Optimum conditions of the pre-treatment (temperature of T=100 degrees C and OMRS-NaOH concentration ratio of about R=20) were determined. The optimum enzymatic conditions determined were as follows: pH of about 5, temperature of T=50 degrees C and enzyme to mass substrate mass ratio E/S=0.1g enzyme (g OMRS)(-1). The maximum saccharification yield obtained at optimum experimental conditions was about 50%. The experimental results agree with Lineweaver Burk's formula for low substrate concentrations. At substrate concentrations greater than 40gdm(-3), inhibitory effects were encountered. The kinetic constants obtained for the batch reactor were K(m)=0.1gdm(-3)min(-1) and V(m)=800gdm(-3). 相似文献
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Rafik Dey Jacques Bodennec Mouh Oulhadj Mameri & Pierre Pernin 《FEMS microbiology letters》2009,290(1):10-17
Legionella pneumophila is known as a facultative intracellular parasite of free-living soil and freshwater amoebae, of which several species have been shown to support the growth of the pathogenic bacteria. We report for the first time the behaviour of two strains (c2c and Z503) of the amoeba Willaertia magna towards different strains of L. pneumophila serogroup 1 and compared it with Acanthamoeba castellanii and Hartmannella vermiformis , known to be L. pneumophila permissive. In contrast to the results seen with other amoebae, W. magna c2c inhibited the growth of one strain of Legionella ( L. pneumophila , Paris), but not of others belonging to the same serogroup ( L. pneumophila , Philadelphia and L. pneumophila , Lens). Also, the different L. pneumophila inhibited cell growth and induced cell death in A. castellanii, H. vermiformis and W. magna Z503 within 3–4 days while W. magna c2c strain remained unaffected even up to 7 days. Electron microscopy demonstrated that the formation of numerous replicative phagosomes observed within Acanthamoeba and Hartmannella is rarely seen in W. magna c2c cocultured with L. pneumophila . Moreover, the morphological differences were observed between L. pneumophila cultured either with Willaertia or other amoebae. These observations show that amoebae are not all equally permissive to L. pneumophila and highlight W. magna c2c as particularly resistant towards some strains of this bacterium. 相似文献
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