全文获取类型
收费全文 | 388篇 |
免费 | 18篇 |
出版年
2022年 | 2篇 |
2021年 | 5篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2018年 | 4篇 |
2017年 | 4篇 |
2016年 | 6篇 |
2015年 | 7篇 |
2014年 | 15篇 |
2013年 | 7篇 |
2012年 | 19篇 |
2011年 | 20篇 |
2010年 | 14篇 |
2009年 | 16篇 |
2008年 | 19篇 |
2007年 | 27篇 |
2006年 | 11篇 |
2005年 | 15篇 |
2004年 | 27篇 |
2003年 | 21篇 |
2002年 | 18篇 |
2001年 | 10篇 |
2000年 | 14篇 |
1999年 | 11篇 |
1998年 | 3篇 |
1997年 | 8篇 |
1996年 | 7篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1993年 | 4篇 |
1992年 | 10篇 |
1991年 | 11篇 |
1990年 | 11篇 |
1989年 | 10篇 |
1988年 | 4篇 |
1987年 | 6篇 |
1986年 | 6篇 |
1985年 | 4篇 |
1984年 | 5篇 |
1983年 | 4篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 4篇 |
1976年 | 1篇 |
1973年 | 1篇 |
1954年 | 1篇 |
排序方式: 共有406条查询结果,搜索用时 296 毫秒
1.
Phylogenetic systematics of the nymphaeales 总被引:2,自引:0,他引:2
Motomi Ito 《Journal of plant research》1987,100(1):17-35
A cladistic analysis was applied to reveal the phylogenetic relationships among the Nymphaeales. Seventeen out of twenty three
characters in gross morphology, anatomy and palynology were analyzed, for their evolutionary polarities. From the results
of the present analysis, the phylogenetic status of each genus and their relationships were clarified: 1)Nelumbo is a distinct taxon and is presumed to have originated from an ancestral stock of the Nymphaeales; 2)Ceratophyllum has a close phylogenetic relationship withCabomba; and 3) in the Nymphaeaceaesensu stricto, Nuphar and the remaining genral constitute a monophyletic group.
A conclusion obtained from the present analysis was that the following three families should be recognized in the Nymphaeales;
Nelumbonaceae Nymphaeaceae, and Ceratophyllaceae. The generaBrasenia andCabomba are traditionally classified in the Nymphaeaceae or in the independent family Cabombaceae. However, they should be included
in the family Ceratophyllaceae. 相似文献
2.
Purification of GTP-binding proteins from bovine brain membranes. Identification of heterogeneity of the alpha-subunit of Go proteins 总被引:3,自引:0,他引:3
Using high-resolution Mono Q column chromatography, we purified 6 distinct peaks of GTP-binding proteins from bovine brain membranes. Five of them consisted of 3 polypeptides with alpha beta gamma-subunits and served as the substrate of islet-activating protein (IAP), pertussis toxin. The other one was purified as alpha-subunit alone and was also ADP-ribosylated by IAP in the presence of beta gamma-subunits. When each alpha-subunit was characterized by immunoblot analysis using various antibodies with defined specificity, the two of them were identified as Gi-1 and Gi-2, and other 4 appeared to be Go or Go-like G proteins. The alpha-subunits of immunologically Go-like proteins were apparently distinguishable from one another on elution profiles from the Mono Q column. Thus, there was a heterogeneity of the alpha-subunit of Go in the brain membranes. 相似文献
3.
The genusCrepidiastrum is distributed in East Asia and includes 7 species. In the Bonin Islands, three species ofCrepidiastrum occur, and all of them are endemic to the islands. For detecting the origin and speciation of these endemic species, electrophoretic
studies have been done in three endemic species of the Bonin Islands as well as in the remaining four species ofCrepidiastrum, andYoungia denticulata which is considered to be closely related toCrepidiastrum.
A total of 386 individuals were sampled from 14 populations. As a result, 17 loci of 10 enzyme systems were resolved and gene
frequencies for each population were calculated. The genetic variability was low in island species, as reported in some oceanic
island plants. Four groups were recognized in the dendrogram generated by the UPGMA method. The Bonin endemics were clustered
together, suggesting a monophyletic origin.C. ameristophyllum andC. linguaefolium were found to be genetically very similar, and this may suggest recent and rapid speciation within the islands. 相似文献
4.
Mitsuyasu Hasebe Rumiko Kofuji Motomi Ito Mikio Kato Kunio Iwatsuki Kunihiko Ueda 《Journal of plant research》1992,105(4):673-679
Partial nucleotide sequences of the large subunit of ribulose-1,5-bisphosphate carboxylase (rubisco) gene (1333 base pairs:
about 90% of the gene) from several seed plants were determined. Phylogenetic trees based on amino acid sequences were inferred
by using the neighbor joining and maximum likelihood methods. The results indicate (1) monophyly of gnetum group (Ephedra, Gnetum, Welwitschia), (2) monophyly of extant gymnosperms containing gnetum group, which contradicts the results of morphological data. 相似文献
5.
A chemotactic peptide stimulated the high-affinity GTPase activity in membrane preparations from guinea pig neutrophils. The enzyme stimulation was inhibited by prior exposure of the membrane-donor cells to islet-activating protein (IAP), pertussis toxin, or by direct incubation of the membrane preparations with its A-protomer (the active peptide) in the presence of NAD. The affinity for the chemotactic peptide binding to its receptors was lowered by guanyl-5'-yl beta, gamma-imidodiphosphate (Gpp(NH)p) reflecting its coupling to the guanine nucleotide regulatory protein in neutrophils. The affinity in the absence of Gpp(NH)p was lower, but the affinity in its presence was not, in the A-protomer-treated membranes than in nontreated membranes. The inhibitory guanine nucleotide regulatory protein of adenylate cyclase (Ni) was purified from rat brain, and reconstituted into the membranes from IAP-treated cells. The reconstitution was very effective in increasing formyl-Met-Leu-Phe-dependent GTPase activity and increasing the chemotactic peptide binding to membranes due to affinity increase. The half-maximal concentration of IAP to inhibit GTPase activity was comparable to that of the toxin to inhibit the cellular arachidonate-releasing response which was well correlated with ADP-ribosylation of a membrane Mr = 41,000 protein (Okajima, F., and Ui, M. (1984) J. Biol. Chem. 259, 13863-13871). It is proposed that the IAP substrate, Ni, couples to the chemotactic peptide receptor and mediates arachidonate-releasing responses in neutrophils, as it mediates adenylate cyclase inhibition in many other cell types. 相似文献
6.
Motomi Ito 《Journal of plant research》1986,99(2):169-184
Floral morphology ofBrasenia schreberi Gmel. andCabomba caroliniana A. Gray was observed chiefly from an anatomical point of view. The receptacle ofB. schreberi is rather flat and a vascular plexus is observable in the mature flower. The vasculature in this plexus is so complex taht
it is not easy to trace its structure in detail. by observation on small buds, it can be seen that the receptacular vasculature
consists of a girdling bundle in the basal area and usually nine receptacular strands from which traces to the petals and
stamens branch off. The vasculature in the receptacle is reconstructed and diagramatically shown as though split longitudinally
and spread out in one plane.
Floral vasculature inCabomba caroliniana is simpler, and is probably related to the smaller number of stamens and carpels. It also has a girdling bundle at the bottom
of receptacle and this vasculature is suggested to be derived by simplification from aBrasenia-type vasculature.
Evidence from floral anatomy suggests that these two genera are closely related. InNymphaea, a vascular plexus in the receptacle is also observed (Moseley, 1961; Ito 1983). The plexus ofBrasenia andNymphaea are not the same in their construction. Nevertheless, their fundamental floral vasculature is comparable and it is preferable
to place them in the same family or same order. 相似文献
7.
A cladistic analysis was used to deduce the phylogenetic relationships within the colonial Volvocales. Forty-one pairs of characters related to gross morphology and ultrastructure of vegetative colonies as well as asexual and sexual reproduction were analyzed based on parsimony, using the PAUP 3.0 computer program, for 25 species belonging to nine volvocacean and goniacean genera of the colonial Volvocales. Chlamydomonas reinhardtii Dangeard was the outgroup. The strict consensus tree indicated the presence of two monophyletic groups, one composed of all the volvocacean species analyzed in this study and the other containing the goniacean species except for the four-celled species Gonium sociale (Dujardin) Warming. In addition, these two groups constitute a large monophyletic group, to which G. sociale is a sister group. A new combination Tetrabaena socialis (Dujardin) Nozaki et Itoh and a new family Tetrabaenaceae Nozaki et Itoh are thus proposed for G. sociale. In addition, the analysis suggests that the volvocacean genera Eudorina and Pleodorina are paraphyletic groups, respectively, and that the monotypic genus Yamagishiella has no autapomorphic characters and represents primitive features of the anisogamous and oogamous genera of the Volvocaceae. Phylogenetic relationships within the Volvocaceae and the Goniaceae, as well as the various modes of sexual reproduction exhibited by these organisms, are discussed on the basis of the analysis. 相似文献
8.
Chloroplast DNA phylogeny of Asian Bamboos (Bambusoideae,Poaceae) and its systematic implication 总被引:1,自引:0,他引:1
The bamboo is usually classified as a subfamily Bambusoideae of Poaceae, and includes approximately 20 genera and 300 species.
To estimate phylogenetic relationships among these genera, we examined restriction site mutations of cpDNA for 16 Asian genera.
In the cladogram obtained, the Bambosoideae was divided into two major lineages, one includingPleioblastus, Pseudosasa, Semiarundinaria, Shibataea, Phyllostachys, Sasa, Sinobambusa, Chimonobambusa, Arthrostylidium, andYushania, and the other consisting ofBambusa, Gigantochloa, Dendrocalamus, Thyrostachys, Melocanna, andSchizostachyum. Monophylly of each clade was supported by 83% and 98% bootstrap probability, respectively. The present result supports monophylly
of Arundinarieae of Potztal's (1964) classical system, but does not support his treatment to recognize Dendrocalameae. 相似文献
9.
Y. Kobayashi Yufuko Takahashi Satoshi Chikayama Motomi Ikeda Nobuhiko Uoshima Shinya Kimura Koji Tanaka Katuya Wada Masaru Ozawa Tatuo Sugano Naoyuki Maruo Motoharu Kondo 《Histochemistry and cell biology》1997,108(2):115-120
We devised a new microfluorometric method for determining the ploidy of megakaryocytes identified immunologically in bone
marrow smears. The smears were immunostained by incubation with mouse monoclonal anti-glycoproteins (GP) IIb antibodies, followed
by fluorescein isothiocyanate-conjugated goat anti-mouse IgG antibodies. They were then stained with 4′,6-diamidino-2-phenylindole
(DAPI). Megakaryocytes were identified by their GPIIb immunofluorescence using a microfluorometer and, after the filters were
changed, their DNA content was assayed by measuring the intensity of DAPI fluorescence. This intensity was shown to be proportional
to the DNA content when the aperture of the objective lens was reduced. We compared these results with those obtained when
megakaryocytes were identified morphologically, using DAPI staining after Wright-Giemsa destaining. In all 12 normal controls,
the ploidy peaks were shown to be 16N by both methods, and the mean ploidy detected by the immunological method was only reduced
0.961 times relative to the estimate from the morphological method. In contrast, in eight myelodysplastic syndrome (MDS) patients,
the ploidy peaks were either 8N or 4N and the mean was reduced by 0.906 times (P=0.018). Thus we could immunologically identify small megakaryocytes which we could not identify morphologically. Therefore,
this method is useful for measuring megakaryocytic ploidy, especially in the pathological megakaryocytes of MDS patients.
Accepted: 29 April 1997 相似文献
10.
U Tomita A Inanobe I Kobayashi K Takahashi M Ui T Katada 《Journal of biochemistry》1991,109(1):184-189
The effects of mastoparan and compound 48/80 on the activities of alpha beta gamma-trimeric GTP-binding proteins (G proteins) were studied with purified Go and Gi-1 which had been reconstituted into phospholipid vesicles. Pertussis toxin-catalyzed ADP-ribosylation of Go or Gi-1 was inhibited by mastoparan or compound 48/80, suggesting that the G proteins were dissociated into their constituent alpha- and beta gamma-subunits in the presence of these compounds. The steady-state rate of GTP hydrolysis catalyzed by Go or Gi-1 was stimulated by the two compounds. Both the stimulations were due to increases in the rate of the GDP-GTP exchange reaction occurring on the G proteins. However, the modes stimulation of the GTPase activity depended on the type of G protein used, and the stimulations caused by the two compounds were differently affected by pertussis toxin-catalyzed ADP-ribosylation of G proteins. Moreover, the mastoparan-induced stimulation of the GTPase activity was partially inhibited by compound 48/80. Thus, the two histamine secretagogues mastoparan and compound 48/80 appear to activate G proteins differently, though they interact with the signal-transducing proteins, at least partly, at a common binding site. 相似文献