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排序方式: 共有77条查询结果,搜索用时 15 毫秒
1.
P Deberghes A M Betbeder F Boisard R Blanc J F Delaby S Krivobok R Steiman F Seigle-Murandi EE Creppy 《Mycotoxin Research》1995,11(1):37-47
The toxicity of ochratoxin A (OTA), a mycotoxin produced by fungi ofAspergillus orPenicillium genera is now well documented. Its nephrotoxicity, immunosuppression, teratogenicity, and carcinogenicity have been widely studied. Physical and biochemical methods have been studied to prevent these toxinogenicAspergillus andPenicillium from producing OTA, and/or to destroy the mycotoxin when already produced in a liquid or a solid medium. Repeated freezing at ? 20?C and thawing at + 26?C aleatory reduce OTA production in a liquid medium. Exposure to UV B for different periods of time is efficient in preventing OTA production in a liquid medium. Gamma-irradiation from 2 to 5 kGy gives good results in preventing the production of OTA or destroying it when already produced. Carboxypeptidase is very efficient at 5 units/50 ml in a liquid medium for cleaving the OTA already produced. 相似文献
2.
V G Mitrofanova Iu D Shenin O A Mirgorodskaia A A Derzhavets E R Matveeva G E Grinberg Iu M Apter L S Golubkova 《Antibiotiki i khimioterapii͡a》1991,36(11):9-11
The component composition of nystatin produced by an improved strain of Streptomyces noursei was determined by HPLC on Milichrom chromatograph (USSR). It was shown that the antibiotic consisted of nystatins A1, A2, A3 and B and admixture substances. The data appeared to be in good agreement with the results of the complex TLC investigation, by using densitometry. The component composition of the samples was evidenced by SIEAP mass spectrometry. Physiochemical and biological characteristics of separate components are presented. 相似文献
3.
M. G. Smaragdov E. I. Saksa A. A. Kudinov N. V. Dement’eva O. V. Mitrofanova K. V. Plemyashov 《Russian Journal of Genetics》2016,52(2):173-179
To form a reference population necessary for genomic selection of dairy cattle, it is important to acquire information on the genetic diversity of the base population. Our report is the first among the studies on breeding of farm animals to implement Wright’s F-statistics for this purpose. Genotyping of animals was performed using BovineSNP50 chip. In total, we genotyped 499 heifers from 13 breeding farms in the Leningrad region. We calculated Weir and Cockerham’s Fst estimate for all pairwise combinations of herds from breeding farms and the values obtained were in the range of 0.016–0.115 with the mean of 0.076 ± 0.002. Theoretical Fst values for the same pairwise combinations of herds were calculated using the ADMIXTURE program. These values were significantly (P < 0.05) higher than Weir and Cockerham’s Fst estimates and fell in the range of 0.063–0.136 with the mean of 0.100 ± 0.001. We discuss the reasons for this discrepancy between the two sets of Fst data. The obtained Fst values were used to identify reliable molecular-genetical differences between the herds. The ADMIXTURE program breaks the pool of 476 heifers into 16 subpopulations, the number of which is close to the number of herds used in the experiment. Results of the comparison between Fst values obtained using SNP markers with published data obtained on microsatellites are in support of the common opinion that microsatellite analysis results in underestimation of Fst values. On the whole, the obtained across-herd Fst values are in the range Fst data reported for cattle breeds. Results of comparison of Fst values with the data on the origin of bulls imported from different countries lead to the conclusion on the expediency of the use of Fst data to assess heterogeneity of the herds. Thus, we have demonstrated that use of Fst data provides the means to assess genetic diversity of cattle herds and is a necessary step in the formation of a reference population for dairy cattle. 相似文献
4.
AP Shoko SM Limbu HA Lamtane MA Kishe-Machumu B Sekadende EE Ulotu 《African Journal of Aquatic Science》2019,44(1):15-24
Aquaculture practices from sub-Saharan Africa are characterised by low production, owing to improper technology. Production can be increased through integrating fish farming with other existing on-farm activities, particularly livestock husbandry. We assessed the role of fish-poultry integration on all male Nile tilapia, Oreochromis niloticus growth performance, yields and economic benefits among smallholder farmers in sub-Saharan Africa, Tanzania. The study also compared phytoplankton species composition, abundance and biomass between the fish-poultry integration and non-integrated system. After 180 days of the experiment, all male O. niloticus cultured under fish-poultry integration exhibited significantly higher growth rates than those in the non-integrated system (p < 0.05). Gross fish yield (GFY), net fish yield (NFY) and net annual yields (NAY) obtained from fish-poultry integration were significantly higher than those from non-integrated system (p < 0.05). Partial enterprise budget analysis revealed that fish-poultry integration was more profitable than the non-integrated system. Moreover, fish-poultry integrated system produced significantly higher phytoplankton abundance and biomass than those from the non-integrated system. Results demonstrate that rural smallholder farmers can achieve higher growth rate, farm net yields and income by integrating all male O. niloticus with other on-farm activities than practising a stand-alone fish culture system. 相似文献
5.
Yegorova N. A. Mitrofanova I. V. Brailko V. A. Grebennikova O. A. Paliy A. E. Stavtseva I. V. 《Russian Journal of Plant Physiology》2019,66(2):326-334
Russian Journal of Plant Physiology - Data on morphogenesis and some physiological and biochemical parameters of explants of five cultivars of Lavandula angustifolia Mill. under long-term... 相似文献
6.
Russian Journal of Genetics - The gene pool of winter common wheat from China maintained in the VIR collection is unique in the variety of alleles of the genes of selection-valuable traits and... 相似文献
7.
Kit Y Mitrofanova EE Shestova OE Kuligina EV Romannikova IV Richter VA 《Ukrainski? biokhimicheski? zhurnal》2000,72(3):73-76
sIgA possessing ability to hydrolyse plasmid DNA to linear forms was purified from human milk by sequential chromatography on protein A-sepharose, DEAE-Fractogel and DNA-cellulose. It was discovered that incubation of sIgA with nuclei of porcine embryo kidney cells permeabilized by Triton X-100 causes formation of electrophoretically mobile forms of nuclear nucleic acids and inhibition of phosphorylation of nuclear proteins. We suppose that sIgA possessing affinity to DNA and endonuclease activity can cause degradation of cell nuclear chromatin. 相似文献
8.
Morozova OV Popova RV Maksimova TG Mitrofanova EE Bakhvalova VN 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2000,(2):54-57
BALB/c mice were immunized with recombinant plasmid DNA pSVK3-ENS1 and pcDNAI-NS3 containing, respectively, genes E-NS1 and NS3 of tick-borne encephalitis (TBE) virus. Antibodies to TBE virus proteins were detected in the blood sera of the immunized animals by the method of the enzyme immunoassay. Though the titers of virus-specific antibodies in the sera of mice immunized with protein vaccines exceeded those registered after immunization with DNA vaccines, essential protective immunity was observed after the use of both vaccines. 相似文献
9.
Nuclear gene trees and the phylogenetic relationships of the mangabeys (Primates: Papionini) 总被引:3,自引:1,他引:2
Phylogenetic relationships of mangabeys within the Old World monkey tribe
Papionini are inferred from analyses of nuclear DNA sequences from five
unlinked loci. The following conclusions are strongly supported, based on
congruence among trees derived for the five separate gene regions: (1)
mangabeys are polyphyletic within the Papionini; (2) Cercocebus is the
sister taxon to the genus Mandrillus; and (3) Lophocebus belongs to a clade
with Papio and Theropithecus, with Papio as its most likely sister taxon.
Morphologically based phylogenies positing mangabey monophyly were
evaluated by mapping the sequences for each locus on these trees. The data
seem to fit these trees poorly in both maximum-parsimony and likelihood
analyses. Incongruence among nuclear gene trees occurred in the
interrelationships among Lophocebus, Papio, and Theropithecus. Several
factors that may account for this incongruence are discussed, including
sampling error, random lineage sorting, and introgression.
相似文献
10.
N. V. Melnikova O. P. Mitrofanova O. A. Liapounova A. M. Kudryavtsev 《Russian Journal of Genetics》2010,46(1):43-49
Genetic diversity for the alleles of gliadin-coding loci was studied with 465 durum wheat accessions from 42 countries. A total of 108 alleles were identified for four loci; 60 alleles were described for the first time. Broad diversity of rare gliadin-coding alleles was observed. The highest genetic diversity was characteristic of durum wheat accessions from the Middle East, Trans-Caucasia, the Pyrenean Peninsula, and the Balkans. Two genetically isolated ancient branches of durum wheat were isolated. A “southern” branch included mostly accessions from the Mediterranean region, the Middle East, and Trans-Caucasia. A “northern” branch included Russian and Ukrainian durum wheat accessions and varieties obtained on their basis. An additional group included durum wheat accessions that had been obtained in several past decades on the basis of the material of international breeding centers (CIMMYT and ICARDA) and had low genetic diversity. 相似文献