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Libik-Konieczny M Surówka E Kuźniak E Nosek M Miszalski Z 《Journal of plant physiology》2011,168(10):1052-1059
Mesembryathemum crystallinum plants performing C3 or CAM (crassulacean acid metabolism) appear to be highly resistant to Botrytis cinerea as well as to Pseudomonas syringae. Fungal hyphae growth was restricted to 48 h post-inoculation (hpi) in both metabolic types and morphology of hyphae differed between those growing in C3 and CAM plants. Growth of bacteria was inhibited significantly 24 hpi in both C3 and CAM plants. B. cinerea and P. syringae infection led to an increase in the concentration of H2O2 in C3 plants 3 hpi, while a decrease in H2O2 content was observed in CAM performing plants. The concentration of H2O2 returned to the control level 24 and 48 hpi. Changes in H2O2 content corresponded with the activity of guaiacol peroxidase (POD), mostly 3 hpi. We noted that its activity decreased significantly in C3 plants and increased in CAM plants in response to inoculation with both pathogens. On the contrary, changes in the activity of CAT did not correlate with H2O2 level. It increased significantly after interaction of C3 plants with B. cinerea or P. syringae, but in CAM performing plants, the activity of this enzyme was unchanged. Inoculation with B. cinerea or P. syringae led to an increase in the total SOD activity in C3 plants while CAM plants did not exhibit changes in the total SOD activity after interaction with both pathogens. In conclusion, the pathogen-induced changes in H2O2 content and in SOD, POD and CAT activities in M. crystallinum leaves, were related to the photosynthetic metabolism type of the stressed plants rather than to the lifestyle of the invading pathogen. 相似文献
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A significantly enhanced production of ethane due to sulphite treatment was observed only in the green fragments of variegated
Chlorophytum comosum leaves but no increase in MDA content was found after sulphite treatment either in the green or in the white leaf fragments.
The activity of SOD showed a tendency to increase while that of catalase significantly decreased only in the green leaf fragments
after SO2 fumigation. The higher level of oxidative damage in the green tissue could probably result from the Haber-Weiss reaction
of generation of HO. 相似文献
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Z. Miszalski A. Kornas K. Gawronska I. Ślesak E. Niewiadomska J. Kruk A. L. Christian E. Fischer-Schliebs R. Krisch U. Lüttge 《Biologia Plantarum》2007,51(1):86-92
The C3-CAM intermediate Clusia minor L. and the C3 obligate Clusia multiflora H.B.K. plants were exposed for 7 d to a combination of drought stress and high irradiance of about 1200 μmol m−2 s−1 for 12 h per day. In both species under these conditions a strong decrease in stomatal conductance was observed at dawn and
dusk. Changes in stomatal behaviour of C. minor were accompanied by only a low nocturnal accumulation of malate and citrate. Thus, in C. minor drought stress applied in combination with high irradiance limited CAM expression, and possibly this is the main reason why
C. minor prefers semi-shaded sites in the field. The mitochondrial MnSOD, in both well watered and stressed plants of two species
showed strong diurnal oscillations with maximum activity at dusk. These oscillations can be explained by the engagement of
mitochondria in dissipation of an excess of reducing equivalents. In plants which are able to carry out CAM metabolism tricarboxylic
acid cycle is expected to be down regulated in the dark period to prevent breakdown of the entire malate and citrate. 相似文献
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S Goraj M Libik-Konieczny E Surówka P Rozpądek A Kalisz A Libik M Nosek P Waligórski Z Miszalski 《Journal of plant physiology》2012,169(12):1158-1164
Differences in the activity of superoxide dismutase, catalase (CAT) and ascorbate peroxidase (APX) as well as in the concentration of ascorbate, tocopherol and hydrogen peroxide (H?O?) were found in leaves from different layers of the Chinese cabbage (Brassica pekinensis (Lour.) Rupr.) head. The youngest chlorophyll-deficient leaves from the most inner layers of the cabbage head were characterized by a high concentration of ascorbate, high activity of iron superoxide dismutase (FeSOD), cooper/zinc superoxide dismutase (Cu/ZnSOD) and a low content of H?O?. On the other hand, activity of CAT, manganese superoxide dismutase (MnSOD) and APX and tocopherol content were highest in chlorophyll-rich leaves from outer parts. The results of this work are interesting from the human nutrition standpoint, as the measured antioxidants have beneficial effects on human health. They can also be utilized to improve storage conditions due to an unequivocal function of antioxidant molecules in maintaining postharvest quality of vegetables. 相似文献
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Zbigniew Miszalski Andrzej Kornas Piotr Rozpądek Elke Fischer-Schliebs Ulrich Lüttge 《Journal of plant physiology》2013
Clusia hilariana Schltdl. is described in literature as an obligate Crassulacean acid metabolism (CAM) species. In the present study we assessed the effect of irradiance with low light (LL, 200 μmol m−2 s−1) and high light (HL, 650–740 μmol m−2 s−1), on the interdependency of citrate and malate diurnal fluctuations. In plants grown at HL CAM-type oscillations of concentration of citrate and malate were obvious. However, at LL daily courses of both acids do not seem to indicate efficient utilization of these compounds as CO2 and NADPH sources. One week after transferring plants from LL to HL decarboxylation of malate was accelerated. Thus, in the CAM plant C. hilariana two independent rhythms of accumulation and decarboxylation of malate and citrate take place, which appear to be related to photosynthesis and respiration, respectively. Non photochemical quenching (NPQ) of photosystem II, especially well expressed during the evening hours was enhanced. Exposure to HL for 7 d activated oxidative stress protection mechanisms such as the interconversion of violaxanthin (V), antheraxanthin (A) and zeaxanthin (Z) (epoxydation/de-epoxydation) measured as epoxydation state (EPS). This was accompanied by a slight increase in the total amount of these pigments. However, all these changes were not observed in plants exposed to HL for only 2 d. Besides violaxanthin cycle components also lutein, which shows a small, but not significant increase, may be involved in dissipating excess light energy in C. hilariana. 相似文献
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In the halophytic plant Mesembryanthemum crystallinum salinity or drought can change the mode of photosynthesis from C3 to crassulacean acid metabolism (CAM). These two stress factors are linked to oxidative stress, however, the induction of CAM by oxidative stress per se is not straightforward. Treatment with high light (HL) did not lead to the induction of CAM, as documented by a low night/day difference in malate level and a low expression of the CAM-related form of phosphoenolcarboxylase (Ppc1), despite causing some oxidative damage (elevated MDA level, malondialdehyde). In contrast to the action of high salinity (0.4 M NaCl), HL treatment did not activate neither the cytosolic NADP-malic enzyme nor the chloroplastic form of NADP-dependent malate dehydrogenase (NADP-MDH). In plastids of HL-treated plants a huge amount of starch was accumulated. This was associated with a weak stimulation of hydrolytic and phosphorolytic starch-degrading enzymes, in contrast to their strong up-regulation under high salinity. It is concluded that HL alone is not able to activate starch degradation necessary for CAM performance. Moreover, in the absence of salinity in C3M. crystallinum plants an age-dependent increase in energy dissipation from PSII was documented under high irradiance, as illustrated by non-photochemical quenching (NPQ). Obtained data suggest that in this halophytic species several photoprotective strategies are strictly salinity-dependent. 相似文献
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E. Kuźniak B. Gabara M. Skłodowska M. Libik-Konieczny Z. Miszalski 《Biologia Plantarum》2011,55(3):423-430
Callus of the halophyte Mesembryanthemum crystallinum was used to study the effect of NaCl on the response to Botrytis cinerea infection. The fungus easily colonized the callus surface and the intercellular spaces. However, in the NaCl-adapted tissues
the incidence of penetration was 67 % lower than in the inoculated control tissue. The modification of the infection pattern
found in the salt-adapted callus could be related to metabolic adaptations to salinity. This was manifested by the enhanced
antioxidant potential of ascorbate, the up-regulated activities of ascorbate peroxidase, as well as guaiacol and syringaldazine
peroxidases together with the increased detoxification capacity of glutathione transferase in the NaCl-adapted callus. The
post-inoculation changes in NaCl-adapted and non-adapted calli were roughly similar and supported the prooxidative nature
of B. cinerea infection. 相似文献