排序方式: 共有5条查询结果,搜索用时 15 毫秒
1
1.
Larina M. V. Finashutina Y. P. Lyzhko N. A. Misyurin V. A. Novoseletsky V. N. Dolgikh D. A. Solopova O. N. Moysenovich A. M. Balabashin D. S. Aliev T. K. Misyurin A. V. Kirpichnikov M. P. 《Russian Journal of Bioorganic Chemistry》2022,48(2):360-371
Russian Journal of Bioorganic Chemistry - The PRAME antigen, which is a significant target for monoclonal antibodies, is a tumor-specific marker that is active at all stages of tumor cell... 相似文献
2.
V. A. Misyurin A. V. Misyurin A. E. Lukina A. A. Krutov L. A. Kesaeva I. N. Soldatova T. V. Akhlynina N. A. Lyzhko O. S. Burova L. F. Morozova I. N. Mikhaylova M. A. Baryshnikova A. Yu. Baryshnikov 《Biochemistry (Moscow) Supplemental Series A: Membrane and Cell Biology》2014,8(3):240-244
A growing number of evidence indicates that cancer-testis antigens (CTA) can be used as specific targets for immune therapy of malignant melanoma. The aim of this study was to provide a basis for selecting the most suitable CTA by analyzing the mRNA expression profile of genes encoding CTA in melanoma cell lines. We used a real-time quantitative PCR to measure the expression level for the following genes: GAGE1, NY-ESO-1, MAGEA1, PASD, SCP1, SEMG1, SPANXA, SSX1, and PRAME. The objects of study were cell lines mel P, mel Si, mel Mtp, mel Il, mel Hn, mel Ibr, and mel Kor obtained from patients diagnosed with disseminated melanoma. We established that the highest frequency of occurrence and the highest expression level had the following genes: GAGE1, NY-ESO-1, MAGEA1, SCP1, SPANXA, SSX1, and PRAME. Their mRNA translation products can be promising candidates for immunotherapy. 相似文献
3.
Solopova O. N. Finashutina Yu. P. Kasatkina N. N. Lyzhko N. A. Turba A. A. Kesaeva L. A. Misyurin V. A. Misyurin A. V. Larina M. V. Aliev T. K. Kirpichnikov M. P. 《Doklady. Biochemistry and biophysics》2021,498(1):199-202
Doklady Biochemistry and Biophysics - Two monoclonal antibodies recognizing non-overlapping epitopes of the PRAME protein were injected into immunocompetent mice to study their influence on the... 相似文献
4.
Sapozhnikova K. A. Misyurin A. V. Pestov N. B. Meleshkina E. G. Oreshkov S. D. Ganzhula E. P. Mikhailova A. S. Korshun V. A. Misyurin V. A. Brylev V. A. 《Russian Journal of Bioorganic Chemistry》2021,47(5):1077-1085
Russian Journal of Bioorganic Chemistry - Determination of the expression level of tumor marker PRAME protein is important both for predicting the course of the disease and for monitoring the... 相似文献
5.
Surin V. L. Luk'yanenko A. V. Karpova I. V. Misyurin A. V. Pustovoit Ya. S. Pivnik A. V. 《Russian Journal of Genetics》2001,37(5):566-572
Porphobilinogen deaminase (PBGD) is a key enzyme of the heme biosynthetic pathway. Defects in the PBGD gene lead to an autosomal dominant disease, acute intermittent porphyria (AIP). Almost all AIP patients with rare exceptions are heterozygous for the defective gene. To date, at least 160 different mutations causing AIP are identified. Extensive investigations along this line are conducted in many countries of the world. In Russia these studies had not been hitherto performed. Here we report the results of molecular genetic examination of four Russian patients with AIP diagnosed from clinical symptoms. By direct sequencing of the PBGD gene or the corresponding cDNA, we have detected four mutations, three of which were not previously encountered in the world population. These are TAAG deletion in intron 7 between positions +2 and +5 (IVS7 2–5 delTAAG); T deletion in the initiation codon ATG of exon 3, and the G for C replacement at position –1 of intron 5 (IVS5 as –1 G–C), which disrupts splicing. In addition, in one female patient, a known deletion CT in codon 68 was revealed. In two patients, expression of PBGD gene alleles was significantly disproportional, so that normal mRNA prevailed in one case and mutant mRNA of nonerythroid type in the other. Deletion in intron 7 was easily detectable due to the formation of a heteroduplex fragment with abnormal electrophoretic mobility directly in PCR. This simple heteroduplex analysis allowed us to exclude AIP carriage in son and daughter of a female patient with the genetic defect. 相似文献
1