全文获取类型
收费全文 | 1522篇 |
免费 | 94篇 |
专业分类
1616篇 |
出版年
2023年 | 8篇 |
2022年 | 23篇 |
2021年 | 30篇 |
2020年 | 20篇 |
2019年 | 24篇 |
2018年 | 38篇 |
2017年 | 28篇 |
2016年 | 53篇 |
2015年 | 53篇 |
2014年 | 65篇 |
2013年 | 77篇 |
2012年 | 125篇 |
2011年 | 117篇 |
2010年 | 75篇 |
2009年 | 73篇 |
2008年 | 106篇 |
2007年 | 77篇 |
2006年 | 104篇 |
2005年 | 84篇 |
2004年 | 50篇 |
2003年 | 54篇 |
2002年 | 44篇 |
2001年 | 21篇 |
2000年 | 13篇 |
1999年 | 15篇 |
1998年 | 12篇 |
1997年 | 14篇 |
1996年 | 12篇 |
1995年 | 6篇 |
1994年 | 15篇 |
1993年 | 9篇 |
1992年 | 14篇 |
1991年 | 13篇 |
1990年 | 10篇 |
1989年 | 13篇 |
1988年 | 9篇 |
1987年 | 8篇 |
1986年 | 5篇 |
1985年 | 5篇 |
1984年 | 6篇 |
1983年 | 5篇 |
1982年 | 9篇 |
1981年 | 6篇 |
1979年 | 6篇 |
1978年 | 5篇 |
1977年 | 6篇 |
1974年 | 7篇 |
1967年 | 6篇 |
1963年 | 5篇 |
1958年 | 5篇 |
排序方式: 共有1616条查询结果,搜索用时 15 毫秒
1.
Nevenka Me?trovi? Martina Pavlek Ana Car Philippe Castagnone-Sereno Pierre Abad Miroslav Plohl 《PloS one》2013,8(6)
Tandemly arrayed non-coding sequences or satellite DNAs (satDNAs) are rapidly evolving segments of eukaryotic genomes, including the centromere, and may raise a genetic barrier that leads to speciation. However, determinants and mechanisms of satDNA sequence dynamics are only partially understood. Sequence analyses of a library of five satDNAs common to the root-knot nematodes Meloidogyne chitwoodi and M. fallax together with a satDNA, which is specific for M. chitwoodi only revealed low sequence identity (32–64%) among them. However, despite sequence differences, two conserved motifs were recovered. One of them turned out to be highly similar to the CENP-B box of human alpha satDNA, identical in 10–12 out of 17 nucleotides. In addition, organization of nematode satDNAs was comparable to that found in alpha satDNA of human and primates, characterized by monomers concurrently arranged in simple and higher-order repeat (HOR) arrays. In contrast to alpha satDNA, phylogenetic clustering of nematode satDNA monomers extracted either from simple or from HOR array indicated frequent shuffling between these two organizational forms. Comparison of homogeneous simple arrays and complex HORs composed of different satDNAs, enabled, for the first time, the identification of conserved motifs as obligatory components of monomer junctions. This observation highlights the role of short motifs in rearrangements, even among highly divergent sequences. Two mechanisms are proposed to be involved in this process, i.e., putative transposition-related cut-and-paste insertions and/or illegitimate recombination. Possibility for involvement of the nematode CENP-B box-like sequence in the transposition-related mechanism and together with previously established similarity of the human CENP-B protein and pogo-like transposases implicate a novel role of the CENP-B box and related sequence motifs in addition to the known function in centromere protein binding. 相似文献
2.
Summary A male fetus of a pregnancy known to be at risk for X-linked mental retardation with fragile site Xq27 was found to be affected by demonstrating the marker X-chromosome in five of 180 (2.8%) of metaphases derived from amniocytes cultured in medium 199. The results were confirmed in fetal lymphocytes (25 of 86 metaphases, i.e. 29%), and fetal fibroblasts (five of 100 metaphases when cultured in medium 199, and 14 of 100 after exposure to methotrexate for 43 h).This work was supported in part by a research grant from the Deutsche Forschungsgemeinschaft 相似文献
3.
4.
5.
J. Macek 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2001,764(1-2)
This review is focused on the different chromatographic strategies for determination of finasteride and its analogues in biological fluids. These compounds are used for the treatment of benign prostatic hyperplasia. Particular attention is paid to high-performance liquid chromatography with spectrophotometric and mass spectrometric detection, the clean-up procedures are also included. The relationships between pharmacokinetics of finasteride, dose administered and required limit of quantitation of the chromatographic assays are discussed. Tandem mass spectrometry is recommended as the detection method for measuring concentrations <1 ng/ml, while cheaper spectrophotometric detection may be selected for determination of higher concentrations. 相似文献
6.
7.
Olga Maslova Oleg Fedevych Nadiia Shuvalova Olena Deryabina Volodymyr Zhovnir Miroslav Novak Peter Kruzliak 《Cell and tissue banking》2016,17(2):335-339
The need for selection of the optimal material for the manufacturing of cardio-patches can be resolved by the use of cryostored autologous pericardial tissue. This short communication is a concise fragment of a large-scale research and demonstrates only the efficiency of cell culturing before and after pericardial preservation in the low temperature conditions. 相似文献
8.
The number of metal atoms contained within a displaceable inorganic component of a metalloprotein was determined by considering X-ray absorption by single crystal samples of holo- and apo-proteins. Since this method is non-destructive, it can be used to determine the number of metal atoms associated with the molecules forming the crystal actually used for X-ray diffraction data collection and subsequent structure solution. The method has been applied to the iron storage protein ferritin, isolated from horse spleen, to give a reliable estimate of the average iron content of the ferritin molecules within the crystal. This value, of around 2000 iron atoms per molecule is consistent with that found for a typical ferritin preparation in solution and suggests non-selectivity of the crystallisation process for ferritin in terms of molecular iron content. 相似文献
9.
Miroslav Pátek Jan Nešvera Jitka Hochmannová 《Applied microbiology and biotechnology》1989,31(1):65-69
Summary Four hybrid plasmids were constructed from the cryptic plasmid pAM330 (from Brevibacterium lactofermentum; 4.5 kb) and the broadhost-range plasmid pGV1106 (9.0 kb; Kmr Smr) isolated from Escherichia coli. All of them were mobilized from E. coli into the Gram-negative methylotrophic bacterium Methylobacillus sp. and two of these constructs (pCEM300 and pCEM400) were transferred by transformation into B. flavum and Corynebacterium glutamicum. Their kanamycin-resistance determinant coming from Gram-negative hosts was expressed in these Gram-positive bacteria. Both pCEM300 and pCEM400 are very stably maintained in B. flavum and represent suitable vectors for gene cloning in coryneform producers of amino acids. 相似文献
10.